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Influenza Virus M2 Protein Mediates ESCRT-Independent Membrane Scission
Open accessAbstract
Summary Many viruses utilize host ESCRT proteins for budding; however, influenza virus budding is thought to be ESCRT-independent. In this study we have found a role for the influenza virus M2 proton-selective ion channel protein in mediating virus budding. We observed that a highly conserved amphipathic helix located within the M2 cytoplasmic tail mediates a cholesterol-dependent alteration in membrane curvature. The 17 amino acid amphipathic helix is sufficient for budding into giant unilamellar vesicles, and mutation of this sequence inhibited budding of transfected M2 protein in vivo. We show that M2 localizes to the neck of budding virions and that mutation of the M2 amphipathic helix results in failure of the virus to undergo membrane scission and virion release. These data suggest that M2 mediates the final steps of budding for influenza viruses, bypassing the need for host ESCRT proteins.
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Cited by
Virus budding and the ESCRT pathway
Votteler, J., & Sundquist, W. I. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.08.012 · Cited by 547
Membrane bending by protein–protein crowding
Stachowiak, J. C., Schmid, E. M., Ryan, C. J., Ann, H. S., Sasaki, D. Y., Sherman, M. B., Geissler, P. L., Fletcher, D. A., & Hayden, C. C. (2012) · Nature Cell Biology · doi:10.1038/ncb2561 · Cited by 537
The role of cholesterol in membrane fusion
Yang, S.-T., Kreutzberger, A. J. B., Lee, J., Kiessling, V., & Tamm, L. K. (2016) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2016.05.003 · Cited by 421
The Race against Protease Activation Defines the Role of ESCRTs in HIV Budding
Bendjennat, M., & Saffarian, S. (2016) · PLOS Pathogens · doi:10.1371/journal.ppat.1005657 · Cited by 45
Importance of viral late domains in budding and release of enveloped RNA viruses
Welker, L., Paillart, J.-C., & Bernacchi, S. (2021) · Viruses · doi:10.3390/v13081559 · Cited by 36
Metadata
- Authors
- Rossman, J. S., Jing, X., Leser, G. P., & Lamb, R. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 142
- Issue
- 6
- Pages
- 902-913
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2037115572
- Cited by
- 513
- References
- 0
- FWCI
- 29.77
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rossman, J. S., Jing, X., Leser, G. P., & Lamb, R. A. (2010). Influenza Virus M2 Protein Mediates ESCRT-Independent Membrane Scission. Cell. https://doi.org/10.1016/j.cell.2010.08.029
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