The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
A Subpopulation of Adult Skeletal Muscle Stem Cells Retains All Template DNA Strands after Cell Division
Open accessAbstract
Satellite cells are adult skeletal muscle stem cells that are quiescent and constitute a poorly defined heterogeneous population. Using transgenic Tg:Pax7-nGFP mice, we show that Pax7-nGFP(Hi) cells are less primed for commitment and have a lower metabolic status and delayed first mitosis compared to Pax7-nGFP(Lo) cells. Pax7-nGFP(Hi) can give rise to Pax7-nGFP(Lo) cells after serial transplantations. Proliferating Pax7-nGFP(Hi) cells exhibit lower metabolic activity, and the majority performs asymmetric DNA segregation during cell division, wherein daughter cells retaining template DNA strands express stem cell markers. Using chromosome orientation-fluorescence in situ hybridization, we demonstrate that all chromatids segregate asymmetrically, whereas Pax7-nGFP(Lo) cells perform random DNA segregation. Therefore, quiescent Pax7-nGFP(Hi) cells represent a reversible dormant stem cell state, and during muscle regeneration, Pax7-nGFP(Hi) cells generate distinct daughter cell fates by asymmetrically segregating template DNA strands to the stem cell. These findings provide major insights into the biology of stem cells that segregate DNA asymmetrically.
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In the citing paper
“There is mounting evidence of asymmetric chromosome segregation in stem cells such as MuSCs17,18,129, ISCs20,130,131 and NSCs132.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [129]
In the citing paper
“In muscle, MuSCs also exist as a heterogeneous quiescent population in adult mice30–33, with changes in heterogeneity with ageing32,34,35.”
From Ageing and rejuvenation of tissue stem cells and their niches (2023) · cites this paper as [31]
Citations
Cites
Pax7 Is Required for the Specification of Myogenic Satellite Cells
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Socializing with the Neighbors
Fuchs, E. V., Tumbar, T., & Guasch, G. (2004) · Cell · doi:10.1016/s0092-8674(04)00255-7 · Cited by 1,737
Mutation selection and the natural history of cancer
Cairns, J. (1975) · Nature · doi:10.1038/255197a0 · Cited by 1,592
Asymmetric self-renewal and commitment of satellite stem cells in muscle
Kuang, S., Kuroda, K., Le Grand, F., & Rudnicki, M. A. (2007) · Cell · doi:10.1016/j.cell.2007.03.044 · Cited by 1,449 · cited 1×
“Compelling evidence points to heterogeneity among quiescent muscle stem cells, regarding their gene expression signature and their functional properties (Biressi et al., 2007; Kuang et al., 2007; Kuang and Rudnicki, 2008; Shinin et al., 2006; Tajbakhsh, 2009).”
Collins, C. A., Olsen, I., Zammit, P. S., Heslop, L., Petrie, A., Partridge, T. A., & Morgan, J. E. (2005) · Cell · doi:10.1016/j.cell.2005.05.010 · Cited by 1,445
Coexistence of Quiescent and Active Adult Stem Cells in Mammals
Li, L., & Clevers, H. (2010) · Science · doi:10.1126/science.1180794 · Cited by 1,247
Direct Isolation of Satellite Cells for Skeletal Muscle Regeneration
Montarras, D., Morgan, J., Collins, C., Relaix, F., Zaffran, S., Cumano, A., Partridge, T., & Buckingham, M. (2005) · Science · doi:10.1126/science.1114758 · Cited by 1,100
Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration
Sambasivan, R., Yao, R., Kissenpfennig, A., Van Wittenberghe, L., Paldi, A., Gayraud-Morel, B., Guenou, H., Malissen, B., Tajbakhsh, S., & Galy, A. (2011) · Development · doi:10.1242/dev.067587 · Cited by 939
Self-citationMuscle satellite cells adopt divergent fates
Zammit, P. S., Golding, J. P., Nagata, Y., Hudon, V., Partridge, T. A., & Beauchamp, J. R. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200312007 · Cited by 924
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Molecular regulation of stem cell quiescence
Cheung, T. H., & Rando, T. A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3591 · Cited by 1,148
Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations
van den Brink, S. C., Sage, F., Vértesy, Á., Spanjaard, B., Peterson-Maduro, J., Baron, C. S., Robin, C., & van Oudenaarden, A. (2017) · Nature Methods · doi:10.1038/nmeth.4437 · Cited by 1,072
Geriatric muscle stem cells switch reversible quiescence into senescence
Sousa-Victor, P., Gutarra, S., García-Prat, L., Rodriguez-Ubreva, J., Ortet, L., Ruiz-Bonilla, V., Jardí, M., Ballestar, E., González, S., Serrano, A. L., et al. (2014) · Nature · doi:10.1038/nature13013 · Cited by 1,010
Relaix, F., & Zammit, P. S. (2012) · Development · doi:10.1242/dev.069088 · Cited by 888
Role of the extracellular matrix in regulating stem cell fate
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Metadata
- Authors
- Rocheteau, P., Gayraud-Morel, B., Siegl-Cachedenier, I., Blasco, M. A., & Tajbakhsh, S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Tajbakhsh, Shahragim
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 148
- Issue
- 1-2
- Pages
- 112-125
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2037334278
- Cited by
- 510
- References
- 57
- Citations by year
- 2026: 16 · 2025: 17 · 2024: 34 · 2023: 45 · 2022: 33 · 2021: 29 · 2020: 31 · 2019: 33 · 2018: 51 · 2017: 41 · 2016: 30 · 2015: 38 · 2014: 39 · 2013: 40 · 2012: 31
- FWCI
- 19.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 563 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-06-BLAN-0039)
- Centre National de la Recherche Scientifique
- Ministerio de Ciencia e Innovación
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- skeletal muscle stem cells, satellite cells, Pax7, stem cell quiescence, cell fate determination, muscle regeneration, cellular metabolism, first mitosis, stem cell heterogeneity, serial transplantation
- MeSH terms
- Animals, Cell Division, Female, Mice, Transgenic, Templates, Genetic, Muscle, Skeletal, Chromosome Segregation, Satellite Cells, Skeletal Muscle, Mice, PAX7 Transcription Factor, Adult Stem Cells
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