The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Single-Molecule mRNA Decay Measurements Reveal Promoter- Regulated mRNA Stability in Yeast
Open accessAbstract
SUMMARY Messenger RNA decay measurements are typically performed on a population of cells. However, this approach cannot reveal sufficient complexity to provide information on mechanisms that may regulate mRNA degradation, possibly on short time scales. To address this deficiency, we measured cell cycle regulated decay in single yeast cells using single-molecule FISH. We found that two genes responsible for mitotic progression, SWI5 and CLB2 exhibit a mitosis-dependent mRNA stability switch. Their transcripts are stable until mitosis when a precipitous decay eliminates the mRNA complement, preventing carry-over into the next cycle. Remarkably, the specificity and timing of decay is entirely regulated by their promoter, independent of specific cis mRNA sequences. The mitotic exit network protein, Dbf2p binds to SWI5 and CLB2 mRNAs co-transcriptionally and regulates their decay. This work reveals the promoter-dependent control of mRNA stability, a novel regulatory mechanism that could be employed by a variety of mRNAs and organisms.
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Global analysis of protein expression in yeast
Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., & Weissman, J. S. (2003) · Nature · doi:10.1038/nature02046 · Cited by 3,750
Visualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504
Self-citationSingle-RNA counting reveals alternative modes of gene expression in yeast
Zenklusen, D., Larson, D. R., & Singer, R. H. (2008) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1514 · Cited by 684
Self-citationA comprehensive, quantitative, and genome-wide model of translation
Siwiak, M., & Zielenkiewicz, P. (2010) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000865 · Cited by 93
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Metadata
- Authors
- Trcek, T., Larson, D. R., Moldón, A., Query, C. C., & Singer, R. H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 147
- Issue
- 7
- Pages
- 1484-1497
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2147028231
- Cited by
- 261
- References
- 4
- FWCI
- 11.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Trcek, T., Larson, D. R., Moldón, A., Query, C. C., & Singer, R. H. (2011). Single-Molecule mRNA Decay Measurements Reveal Promoter- Regulated mRNA Stability in Yeast. Cell. https://doi.org/10.1016/j.cell.2011.11.051
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