The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Real-Time Imaging of Translation on Single mRNA Transcripts in Live Cells
Open accessAbstract
SUMMARY Translation is under tight spatial and temporal controls to ensure protein production in the right time and place in cells. Methods that allow real-time, high-resolution visualization of translation in live cells are essential for understanding the spatiotemporal dynamics of translation regulation. Based on multivalent fluorescence amplification of the nascent polypeptide signal, we develop a method to image translation on individual mRNA molecules in real time in live cells, allowing direct visualization of translation events at the translation sites. Using this approach, we monitor transient changes of translation dynamics in responses to environmental stresses, capture distinct mobilities of individual polysomes in different subcellular compartments, and detect 3’UTR-dependent local translation and active transport of polysomes in dendrites of primary neurons.
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Self-citation
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Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs
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The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells
Horvathova, I., Voigt, F., Kotrys, A. V., Zhan, Y., Artus-Revel, C. G., Eglinger, J., Stadler, M. B., Giorgetti, L., & Chao, J. A. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.030 · Cited by 225
Plasticity of Spine Structure: Local Signaling, Translation and Cytoskeletal Reorganization
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Voigt, F., Zhang, H., Cui, X. A., Triebold, D., Liu, A. X., Eglinger, J., Lee, E. S., Chao, J. A., & Palazzo, A. F. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.12.008 · Cited by 119
Metadata
- Authors
- Wang, C., Han, B., Zhou, R., & Zhuang, X.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 165
- Issue
- 4
- Pages
- 990-1001
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2346053509
- Cited by
- 359
- References
- 64
- FWCI
- 22.12
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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