The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold
Open accessAbstract
Summary The multi-protein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specify the position of the kinetochore and provide a scaffold for kinetochore formation remain key goals. Here, we demonstrate that the centromeric histone-fold containing CENP-T-W and CENP-S-X complexes co-assemble to form a stable CENP-T-W-S-X heterotetramer. High-resolution structural analysis of the individual complexes and the heterotetramer reveals similarity to other histone fold-containing complexes including canonical histones within a nucleosome. The CENP-T-W-S-X heterotetramer binds to and supercoils DNA. Mutants designed to compromise heterotetramerization or the DNA-protein contacts around the heterotetramer strongly reduce the DNA binding and supercoiling activities in vitro and compromise kinetochore assembly in vivo. These data suggest that the CENP-T-W-S-X complex forms a unique nucleosome-like structure to generate contacts with DNA, extending the “histone code” beyond canonical nucleosome proteins.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
PHENIX : a comprehensive Python-based system for macromolecular structure solution
Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716
Cited by
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome
Yatskevich, S., Muir, K. W., Bellini, D., Zhang, Z., Yang, J., Tischer, T., Predin, M., Dendooven, T., McLaughlin, S. H., & Barford, D. (2022) · Science · doi:10.1126/science.abn3810 · Cited by 120
Tilocca, B., Balmas, V., Hassan, Z. U., Jaoua, S., & Migheli, Q. (2019) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2019.108265 · Cited by 71
Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
Dendooven, T., Zhang, Z., Yang, J., McLaughlin, S. H., Schwab, J., Scheres, S. H. W., Yatskevich, S., & Barford, D. (2023) · Science Advances · doi:10.1126/sciadv.adg7480 · Cited by 58
Insects evolved a monomeric histone-fold domain in the CENP-T protein family
Sankaranarayanan, S. R., Ulmer, J., Mørch, A., Ali-Ahmad, A., Sekulić, N., & Drinnenberg, I. A. (2025) · EMBO Reports · doi:10.1038/s44319-025-00603-5 · Cited by 2
Metadata
- Authors
- Nishino, T., Takeuchi, K., Gascoigne, K. E., Suzuki, A., Hori, T., Oyama, T., Morikawa, K., Cheeseman, I. M., & Fukagawa, T.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 148
- Issue
- 3
- Pages
- 487-501
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2010280142
- Cited by
- 263
- References
- 1
- FWCI
- 12.36
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold. https://app.cochat.ai/papers/10.1016/j.cell.2011.11.061
- The paper
- Nishino, T., Takeuchi, K., Gascoigne, K. E., Suzuki, A., Hori, T., Oyama, T., Morikawa, K., Cheeseman, I. M., & Fukagawa, T. (2012). CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold. Cell. https://doi.org/10.1016/j.cell.2011.11.061
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
