The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
Open accessAbstract
Summary Signals in many biological processes can be amplified by recruiting multiple copies of regulatory proteins to a site of action. Harnessing this principle, we have developed a novel protein scaffold, a repeating peptide array termed SunTag, which can recruit multiple copies of an antibody-fusion protein. We show that the SunTag can recruit up to 24 copies of GFP, thereby enabling long-term imaging of single protein molecules in living cells. We also use the SunTag to create a potent synthetic transcription factor by recruiting multiple copies of a transcriptional activation domain to a nuclease-deficient CRISPR/Cas9 protein and demonstrate strong activation of endogenous gene expression and re-engineered cell behavior with this system. Thus, the SunTag provides a versatile platform for multimerizing proteins on a target protein scaffold and is likely to have many applications in imaging and in controlling biological outputs.
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CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Self-citationMali, P., Aach, J., Stranges, P. B., Esvelt, K. M., Moosburner, M., Kosuri, S., Yang, L., & Church, G. M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2675 · Cited by 1,752 · cited 8×
“…Cas9 (dCas9), which can be targeted to any sequence in the mammalian genome using sequence-specific small guide RNAs (sgRNAs) (Cheng et al., 2013; Farzadfard et al., 2013; Gilbert et al., 2013; Hu et al., 2014; Kearns et al., 2014; Maeder et al., 2013; Mali et al., 2013; PerezPinera et al., 2013).”
InfluentialTracking the ends: a dynamic protein network controls the fate of microtubule tips
Akhmanova, A., & Steinmetz, M. O. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2369 · Cited by 1,047
High-affinity binders selected from designed ankyrin repeat protein libraries
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Tanenbaum, M. E., Macurek, L., van der Vaart, B., Galli, M., Akhmanova, A., & Medema, R. H. (2011) · Current Biology · doi:10.1016/j.cub.2011.07.017 · Cited by 148
Self-citationKif18B interacts with EB1 and controls astral microtubule length during mitosis
Stout, J. R., Yount, A. L., Powers, J. A., LeBlanc, C., Ems-McClung, S. C., & Walczak, C. E. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-04-0363 · Cited by 111
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Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728
Self-citationBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675
Highly efficient Cas9-mediated transcriptional programming
Chavez, A., Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., P R Iyer, E., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3312 · Cited by 1,622
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Metadata
- Authors
- Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J. S., & Vale, R. D.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 159
- Issue
- 3
- Pages
- 635-646
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2169373177
- Cited by
- 1,580
- References
- 7
- FWCI
- 52.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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