The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Asymmetric Unwrapping of Nucleosomes under Tension Directed by DNA Local Flexibility
Open accessAbstract
Summary Dynamics of the nucleosome and exposure of nucleosomal DNA play key roles in many nuclear processes but local dynamics of the nucleosome and its modulation by DNA sequence are poorly understood. Using single-molecule assays we observed that the nucleosome can unwrap asymmetrically and directionally under force. The relative DNA flexibility of the inner quarters of nucleosomal DNA controls the unwrapping direction such that the nucleosome unwraps from the stiffer side. If the DNA flexibility is similar on two sides, it stochastically unwraps from either side. The two ends of the nucleosome are orchestrated such that the opening of one end helps to stabilize the other end, providing a mechanism to amplify even small differences in flexibility to a large asymmetry in nucleosome stability. Our discovery of DNA flexibility as a critical factor for nucleosome dynamics and mechanical stability suggests a novel mechanism of gene regulation by DNA sequence and modifications.
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Cites
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Histone Core Phosphorylation Regulates DNA Accessibility
Brehove, M., Wang, T., North, J., Luo, Y., Dreher, S. J., Shimko, J. C., Ottesen, J. J., Luger, K., & Poirier, M. G. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.661363 · Cited by 93
Histone divergence in trypanosomes results in unique alterations to nucleosome structure
Deák, G., Wapenaar, H., Sandoval, G., Chen, R., Taylor, M. R. D., Burdett, H., Watson, J. A., Tuijtel, M. W., Webb, S., & Wilson, M. D. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad577 · Cited by 21
Metadata
- Authors
- Ngo, T. T. M., Zhang, Q., Zhou, R., Yodh, J. G., & Ha, T.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 160
- Issue
- 6
- Pages
- 1135-1144
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2162563461
- Cited by
- 323
- References
- 49
- FWCI
- 11.45
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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