The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation
Open accessAbstract
SUMMARY Sensing of lipopolysaccharide (LPS) in the cytosol triggers caspase-11 activation and is central to host defense against Gram-negative bacterial infections and to the pathogenesis of sepsis. Most Gram-negative bacteria that activate caspase-11 however are not cytosolic and the mechanism by which LPS from these bacteria gains access to caspase-11 in the cytosol remains elusive. Here we identify outer membrane vesicles (OMV) produced by Gram-negative bacteria as a vehicle that delivers LPS into the cytosol triggering caspase-11-dependent effector responses in vitro and in vivo. OMV are internalized via endocytosis, and LPS is released into the cytosol from early endosomes. The use of hypovesiculating bacterial mutants, compromised in their ability to generate OMV, reveal the importance of OMV in mediating the cytosolic localization of LPS. Collectively, these findings demonstrate a critical role for OMV in enabling the cytosolic entry of LPS and consequently caspase-11 activation during Gram-negative bacterial infections.
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Cites
Cyclic GMP-AMP Synthase Is a Cytosolic DNA Sensor That Activates the Type I Interferon Pathway
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Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
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Non-canonical inflammasome activation targets caspase-11
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Inflammatory caspases are innate immune receptors for intracellular LPS
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Molecular mechanism and physiological functions of clathrin-mediated endocytosis
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The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses
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Self-citationOuter Membrane Vesicles of a Human Commensal Mediate Immune Regulation and Disease Protection
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Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases
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Cited by
TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling
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The NLRP3 inflammasome: Mechanism of action, role in disease and therapies
Wang, L., & Hauenstein, A. V. (2020) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2020.100889 · Cited by 426
Wu, K., Yuan, Y., Yu, H., Dai, X., Wang, S., Sun, Z., Wang, F., Fei, H., Lin, Q., Jiang, H., et al. (2020) · Blood · doi:10.1182/blood.2019003990 · Cited by 345
Yang, X., Cheng, X., Tang, Y., Qiu, X., Wang, Y., Kang, H., Wu, J., Wang, Z., Liu, Y., Chen, F., et al. (2019) · Immunity · doi:10.1016/j.immuni.2019.11.005 · Cited by 325
A Journey from Structure to Function of Bacterial Lipopolysaccharides
Di Lorenzo, F., Duda, K. A., Lanzetta, R., Silipo, A., De Castro, C., & Molinaro, A. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01321 · Cited by 322
Metadata
- Authors
- Vanaja, S. K., Russo, A. J., Behl, B., Banerjee, I., Yankova, M., Deshmukh, S. D., & Rathinam, V. A. K.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 165
- Issue
- 5
- Pages
- 1106-1119
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2345407464
- Cited by
- 727
- References
- 9
- FWCI
- 25.73
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Vanaja, S. K., Russo, A. J., Behl, B., Banerjee, I., Yankova, M., Deshmukh, S. D., & Rathinam, V. A. K. (2016). Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation. Cell. https://doi.org/10.1016/j.cell.2016.04.015
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