Molecular mechanism and physiological functions of clathrin-mediated endocytosis
Abstract
Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat. It is fundamental to neurotransmission, signal transduction and the regulation of many plasma membrane activities and is thus essential to higher eukaryotic life. Morphological stages of vesicle formation are mirrored by progression through various protein modules (complexes). The process involves the formation of a putative FCH domain only (FCHO) initiation complex, which matures through adaptor protein 2 (AP2)-dependent cargo selection, and subsequent coat building, dynamin-mediated scission and finally auxilin- and heat shock cognate 70 (HSC70)-dependent uncoating. Some modules can be used in other pathways, and additions or substitutions confer cell specificity and adaptability.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
GWAS of Alzheimer's disease patients revealed deregulated expression of genes PICALM, BIN-1, and sorLA, which are essentially involved in clathrin-mediated endocytosis.
“The genome-wide association studies (GWAS) of AD patients revealed that the deregulated expression of genes PICALM, BIN-1, and sorLA, which are essentially involved in clathrin-mediated endocytosis [156,157].”
From Insights of Endocytosis Signaling in Health and Disease (2023) · cites this paper as [156]
Cites
Molecular anatomy of a trafficking organelle
Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., Urlaub, H., Schenck, S., Brügger, B., Ringler, P., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.030 · Cited by 2,373 · see citing works
Systematic sequencing of renal carcinoma reveals inactivation of histone modifying genes
Dalgliesh, G. L., Furge, K., Greenman, C., Chen, L., Bignell, G., Butler, A., Davies, H., Edkins, S., Hardy, C., Latimer, C., et al. (2010) · Nature · doi:10.1038/nature08672 · Cited by 1,173 · see citing works
A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2005) · Cell · doi:10.1016/j.cell.2005.09.024 · Cited by 726 · cited 1× · see citing works
“Pathway modularity At the heart of clathrin-coated vesicle formation is a flexible network of interactions, which can be thought of as being made up of different module”
BackgroundNicot, A.-S., Toussaint, A., Tosch, V., Kretz, C., Wallgren-Pettersson, C., Iwarsson, E., Kingston, H., Garnier, J.-M., Biancalana, V., Oldfors, A., et al. (2007) · Nature Genetics · doi:10.1038/ng2086 · Cited by 434 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Toll-like Receptors and the Control of Immunity
Fitzgerald, K. A., & Kagan, J. C. (2020) · Cell · doi:10.1016/j.cell.2020.02.041 · Cited by 2,168 · see citing works
Mechanisms and regulation of endothelial VEGF receptor signalling
Simons, M., Gordon, E., & Claesson-Welsh, L. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.87 · Cited by 1,498 · see citing works
Block copolymer micelles in nanomedicine applications
Cabral, H., Miyata, K., Osada, K., & Kataoka, K. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00199 · Cited by 1,309 · see citing works
Bendor, J. T., Logan, T. P., & Edwards, R. H. (2013) · Neuron · doi:10.1016/j.neuron.2013.09.004 · Cited by 825 · see citing works
Ultrafast endocytosis at mouse hippocampal synapses
Watanabe, S., Rost, B. R., Camacho-Pérez, M., Davis, M. W., Söhl-Kielczynski, B., Rosenmund, C., & Jorgensen, E. M. (2013) · Nature · doi:10.1038/nature12809 · Cited by 607 · see citing works
Endophilin marks and controls a clathrin-independent endocytic pathway
Boucrot, E., Ferreira, A. P. A., Almeida-Souza, L., Debard, S., Vallis, Y., Howard, G., Bertot, L., Sauvonnet, N., & McMahon, H. T. (2015) · Nature · doi:10.1038/nature14067 · Cited by 542 · see citing works
Self-citationGenetics and biology of pancreatic ductal adenocarcinoma
Ying, H., Dey, P., Yao, W., Kimmelman, A. C., Draetta, G. F., Maitra, A., & DePinho, R. A. (2016) · Genes & Development · doi:10.1101/gad.275776.115 · Cited by 527 · see citing works
Axl Mediates ZIKA Virus Entry in Human Glial Cells and Modulates Innate Immune Responses
Meertens, L., Labeau, A., Dejarnac, O., Cipriani, S., Sinigaglia, L., Bonnet-Madin, L., Le Charpentier, T., Hafirassou, M. L., Zamborlini, A., Cao-Lormeau, V.-M., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2016.12.045 · Cited by 450 · see citing works
Saheki, Y., & De Camilli, P. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005645 · Cited by 457 · see citing works
The First Five Seconds in the Life of a Clathrin-Coated Pit
Cocucci, E., Aguet, F., Boulant, S., & Kirchhausen, T. (2012) · Cell · doi:10.1016/j.cell.2012.05.047 · Cited by 391 · see citing works
Nutrient acquisition strategies of mammalian cells
Palm, W., & Thompson, C. B. (2017) · Nature · doi:10.1038/nature22379 · Cited by 388 · see citing works
A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation
Li, X., Rydzewski, N., Hider, A., Zhang, X., Yang, J., Wang, W., Gao, Q., Cheng, X., & Xu, H. (2016) · Nature Cell Biology · doi:10.1038/ncb3324 · Cited by 387 · see citing works
Synaptic vesicle recycling: steps and principles
Rizzoli, S. O. (2014) · The EMBO Journal · doi:10.1002/embj.201386357 · Cited by 317 · see citing works
Phosphoinositides in Control of Membrane Dynamics
Schink, K. O., Tan, K.-W., & Stenmark, H. (2016) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-111315-125349 · Cited by 283 · see citing works
Current Knowledge on Exosome Biogenesis, Cargo-Sorting Mechanism and Therapeutic Implications
Xie, S., Zhang, Q., & Jiang, L. (2022) · Membranes · doi:10.3390/membranes12050498 · Cited by 262 · see citing works
High throughput error corrected Nanopore single cell transcriptome sequencing
Lebrigand, K., Magnone, V., Barbry, P., & Waldmann, R. (2020) · Nature Communications · doi:10.1038/s41467-020-17800-6 · Cited by 236 · see citing works
Meinecke, M., Boucrot, E., Camdere, G., Hon, W.-C., Mittal, R., & McMahon, H. T. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.444869 · Cited by 155 · see citing works
Self-citationVps34 Deficiency Reveals the Importance of Endocytosis for Podocyte Homeostasis
Bechtel, W., Helmstädter, M., Balica, J., Hartleben, B., Kiefer, B., Hrnjic, F., Schell, C., Kretz, O., Liu, S., Geist, F., et al. (2013) · Journal of the American Society of Nephrology · doi:10.1681/asn.2012070700 · Cited by 132 · see citing works
The endocytic pathway in microglia during health, aging and Alzheimer’s disease
Solé-Domènech, S., Cruz, D. L., Capetillo-Zarate, E., & Maxfield, F. R. (2016) · Ageing Research Reviews · doi:10.1016/j.arr.2016.07.002 · Cited by 133 · see citing works
Liu, D., Hong, Y., Li, Y., Hu, C., Yip, T.-C., Yu, W.-K., Zhu, Y., Fong, C.-C., Wang, W., Au, S.-K., et al. (2020) · Theranostics · doi:10.7150/thno.38989 · Cited by 125 · see citing works
Spoiled for Choice: Diverse Endocytic Pathways Function at the Cell Surface
Thottacherry, J. J., Sathe, M., Prabhakara, C., & Mayor, S. (2019) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100617-062710 · Cited by 122 · see citing works
Nanoparticle-based technologies for retinal gene therapy
Adijanto, J., & Naash, M. I. (2015) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2014.12.028 · Cited by 107 · see citing works
Fungal invasion of epithelial cells
Yang, W., Yan, L., Wu, C., Zhao, X., & Tang, J. (2014) · Microbiological Research · doi:10.1016/j.micres.2014.02.013 · Cited by 79 · see citing works
Green, J. A., Schmid, C. L., Bley, E., Monsma, P. C., Brown, A., Bohn, L. M., & Mykytyn, K. (2016) · Molecular and Cellular Biology · doi:10.1128/mcb.00765-15 · Cited by 67 · see citing works
A non-bilaterian perspective on the development and evolution of animal digestive systems
Steinmetz, P. R. H. (2019) · Cell and Tissue Research · doi:10.1007/s00441-019-03075-x · Cited by 67 · see citing works
Insights of Endocytosis Signaling in Health and Disease
Pathak, C., Vaidya, F. U., Waghela, B. N., Jaiswara, P. K., Gupta, V. K., Kumar, A., Rajendran, B. K., & Ranjan, K. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032971 · Cited by 59 · see citing works
Conrad, R., Stölting, G., Hendriks, J., Ruello, G., Kortzak, D., Jordan, N., Gensch, T., & Hidalgo, P. (2018) · iScience · doi:10.1016/j.isci.2018.08.012 · Cited by 35 · see citing works
Synthesis and Characterization of Fatty Acid Conjugates of Niacin and Salicylic Acid
Vu, C. B., Bemis, J. E., Benson, E., Bista, P., Carney, D., Fahrner, R., Lee, D., Liu, F., Lonkar, P., Milne, J. C., et al. (2016) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.5b01961 · Cited by 27 · see citing works
Jaye, S., Sandau, U. S., & Saugstad, J. A. (2024) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2024.1378576 · Cited by 26 · see citing works
pH-Responsive Natural Polymers as Versatile Carriers for Drug Delivery in Breast Cancer: A Review
Velapure, P. D., Chakote, S. R., Kansal, D., Barooah, A., Pawde, D. M., & Bobade, C. D. (2026) · Biomedical Materials & Devices · doi:10.1007/s44174-025-00427-w · Cited by 5 · see citing works
CCDC32 stabilizes clathrin-coated pits and drives their invagination
Yang, Z., Yang, C., Huang, Z., Xu, P., Li, Y., Han, L., Peng, L., Wei, X., Pak, J. E., Svitkina, T., et al. (2026) · eLife · doi:10.7554/elife.107039.3 · Cited by 3 · see citing works
Synaptic Vesicle Recycling at the Developing Presynapse
Kim, N., & Cousin, M. A. (2025) · Journal of Neurochemistry · doi:10.1111/jnc.70206 · Cited by 2 · see citing works
“A key aspect of CME is the clustering of SV cargo proteins by adaptor protein complex 2 (AP2), which both identifies cargo for retrieval and the recruitment of subsequent clathrin coat proteins (Kelly and Owen 2011; McMahon and Boucrot 2011; Robinson 2004).”
- Authors
- McMahon, H. T., & Boucrot, E.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- Corresponding author
- McMahon, Harvey T.
- Boucrot, Emmanuel
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2011-07-22
- Volume
- 12
- Issue
- 8
- Pages
- 517-533
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- OpenAlex
- W2023863818
- Cited by
- 2,223
- References
- 4
- Citations by year
- 2026: 110 · 2025: 125 · 2024: 135 · 2023: 109 · 2022: 149 · 2021: 175 · 2020: 155 · 2019: 151 · 2018: 169 · 2017: 172 · 2016: 147 · 2015: 153 · 2014: 179 · 2013: 177 · 2012: 105
- FWCI
- 13.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- clathrin-mediated endocytosis, clathrin coat, adaptor proteins, AP-2, auxilin, Hsc70, vesicle formation, cargo selection, signal termination, neurotransmission, signal transduction, endocytic pathway
- MeSH terms
- Actins, Animals, Clathrin, Endocytosis, Humans, Models, Biological, Mutation, Neoplasms, Synaptic Vesicles, Signal Transduction, Clathrin-Coated Vesicles, Adaptor Proteins, Vesicular Transport, Adaptor Protein Complex 2, Dynamins, RNA Interference
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
