The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease
Open accessAbstract
SUMMARY C2c1 is a newly-identified guide RNA-mediated type V-B CRISPR-Cas endonuclease that site-specifically targets and cleaves both strands of target DNA. We have determined crystal structures of Alicyclobacillus acidoterrestris C2c1 (AacC2c1) bound to sgRNA as a binary complex and to target DNAs as ternary complexes, thereby capturing catalytically competent conformations of AacC2c1 with both target and non-target DNA strands independently positioned within a single RuvC catalytic pocket. Moreover, C2c1-mediated cleavage results in a staggered seven-nucleotide break of target DNA. crRNA adopts a pre-ordered five-nucleotide A-form seed sequence in the binary complex, with release of an inserted tryptophan, facilitating zippering-up of 20-bp RNA-DNA heteroduplex on ternary complex formation. Notably, the PAM-interacting cleft adopts a “locked” conformation on ternary complex formation. Structural comparison of C2c1 ternary complexes with their Cas9 and Cpf1 counterparts highlights the diverse mechanisms adopted by these distinct CRISPR-Cas9 systems, thereby broadening and enhancing their applicability as genome editing tools.
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CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
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Crystal structure of Cas9 in complex with guide RNA and target DNA
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Cited by
Diversity, classification and evolution of CRISPR-Cas systems
Koonin, E. V., Makarova, K. S., & Zhang, F. (2017) · Current Opinion in Microbiology · doi:10.1016/j.mib.2017.05.008 · Cited by 1,423
Diversity and evolution of class 2 CRISPR–Cas systems
Shmakov, S., Smargon, A., Scott, D., Cox, D., Pyzocha, N., Yan, W., Abudayyeh, O. O., Gootenberg, J. S., Makarova, K. S., Wolf, Y. I., et al. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.184 · Cited by 1,029
Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a
Swarts, D. C., van der Oost, J., & Jinek, M. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.03.016 · Cited by 608
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a
Liu, L., Li, X., Ma, J., Li, Z., You, L., Wang, J., Wang, M., Zhang, X., & Wang, Y. (2017) · Cell · doi:10.1016/j.cell.2017.06.050 · Cited by 538
Origins and evolution of CRISPR-Cas systems
Koonin, E. V., & Makarova, K. S. (2019) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2018.0087 · Cited by 494
Metadata
- Authors
- Yang, H., Gao, P., Rajashankar, K. R., & Patel, D. J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-12
- Volume
- 167
- Issue
- 7
- Pages
- 1814-1828.e12
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2566705644
- Cited by
- 277
- References
- 4
- FWCI
- 10.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yang, H., Gao, P., Rajashankar, K. R., & Patel, D. J. (2016). PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease. Cell. https://doi.org/10.1016/j.cell.2016.11.053
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