CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
Open accessAbstract
CRISPR/Cas systems constitute a widespread class of immunity systems that protect bacteria and archaea against phages and plasmids, and commonly use repeat/spacer-derived short crRNAs to silence foreign nucleic acids in a sequence-specific manner. Although the maturation of crRNAs represents a key event in CRISPR activation, the responsible endoribonucleases (CasE, Cas6, Csy4) are missing in many CRISPR/Cas subtypes. Here, differential RNA sequencing of the human pathogen Streptococcus pyogenes uncovered tracrRNA, a trans-encoded small RNA with 24-nucleotide complementarity to the repeat regions of crRNA precursor transcripts. We show that tracrRNA directs the maturation of crRNAs by the activities of the widely conserved endogenous RNase III and the CRISPR-associated Csn1 protein; all these components are essential to protect S. pyogenes against prophage-derived DNA. Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders.
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Allicin shows senolytic activity when administered to breast cancer cells.
“For example, while allicin shows senolytic activity when administered to breast cancer cells, alliin instead behaves as a pro-senolytic compound in the same conditions [211].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [211]
Alliin behaves as a pro-senolytic compound in breast cancer cells.
“For example, while allicin shows senolytic activity when administered to breast cancer cells, alliin instead behaves as a pro-senolytic compound in the same conditions [211].”
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“CRISPR/Cas9 system derived from Streptococcus pyogenes is in the Type II – A CRISPR/Cas system consisting of Cas9 nuclease and two short RNAs, crispr RNA (crRNA) and trans activating crRNA (tracrRNA) (Deltcheva et al., 2011).”
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- Authors
- Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E.
- Affiliations
- Umeå Centre for Microbial Research (SE)
- University of Vienna (AT)
- University of Würzburg (DE)
- Corresponding author
- Charpentier, Emmanuelle
- ORCID
- Venue
- Nature
- Published
- 2011-03
- Volume
- 471
- Issue
- 7340
- Pages
- 602-607
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC3070239
- OpenAlex
- W2120503144
- Cited by
- 2,563
- References
- 67
- Citations by year
- 2026: 77 · 2025: 143 · 2024: 177 · 2023: 182 · 2022: 221 · 2021: 250 · 2020: 225 · 2019: 207 · 2018: 203 · 2017: 176 · 2016: 195 · 2015: 164 · 2014: 139 · 2013: 116 · 2012: 69
- FWCI
- 44.24
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Keywords
- CRISPR-Cas, tracrRNA, RNase III, Streptococcus pyogenes
- MeSH terms
- Bacterial Proteins, DNA, Viral, Escherichia coli, Models, Biological, RNA Precursors, RNA Processing, Post-Transcriptional, RNA, Bacterial, Streptococcus pyogenes, Conserved Sequence, Prophages, Ribonuclease III, RNA, Small Untranslated
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