The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis
Open accessAbstract
Engineering microorganisms for production of fuels and chemicals often requires major re-programming of metabolism to ensure high flux toward the product of interest. This is challenging, as millions of years of evolution have resulted in establishment of tight regulation of metabolism for optimal growth in the organism's natural habitat. Here, we show through metabolic engineering that it is possible to alter the metabolism of Saccharomyces cerevisiae from traditional ethanol fermentation to a pure lipogenesis metabolism, resulting in high-level production of free fatty acids. Through metabolic engineering and process design, we altered subcellular metabolic trafficking, fine-tuned NADPH and ATP supply, and decreased carbon flux to biomass, enabling production of 33.4 g/L extracellular free fatty acids. We further demonstrate that lipogenesis metabolism can replace ethanol fermentation by deletion of pyruvate decarboxylase enzymes followed by adaptive laboratory evolution. Genome sequencing of evolved strains showed that pyruvate kinase mutations were essential for this phenotype.
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Self-citationA Mitochondrial Pyruvate Carrier Required for Pyruvate Uptake in Yeast, Drosophila , and Humans
Bricker, D. K., Taylor, E. B., Schell, J. C., Orsak, T., Boutron, A., Chen, Y.-C., Cox, J. E., Cardon, C. M., Van Vranken, J. G., Dephoure, N., et al. (2012) · Science · doi:10.1126/science.1218099 · Cited by 843
Pyruvate Metabolism inSaccharomyces cerevisiae
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Cited by
Włodarczyk, A., Selão, T. T., Norling, B., & Nixon, P. J. (2020) · Communications Biology · doi:10.1038/s42003-020-0910-8 · Cited by 192
Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals
Yan, Q., & Pfleger, B. F. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.009 · Cited by 105
Application of cofactors in the regulation of microbial metabolism: A state of the art review
Sun, Y., Zhang, T., Lu, B., Li, X., & Jiang, L. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1145784 · Cited by 68
Yeast metabolic innovations emerged via expanded metabolic network and gene positive selection
Lu, H., Li, F., Yuan, L., Domenzain, I., Yu, R., Wang, H., Li, G., Chen, Y., Ji, B., Kerkhoven, E. J., et al. (2021) · Molecular Systems Biology · doi:10.15252/msb.202110427 · Cited by 44
Self-citationKamrad, S., Grossbach, J., Rodríguez‐López, M., Mülleder, M., Townsend, S., Cappelletti, V., Stojanovski, G., Correia‐Melo, C., Picotti, P., Beyer, A., et al. (2020) · Molecular Systems Biology · doi:10.15252/msb.20199270 · Cited by 40
Metadata
- Authors
- Yu, T., Zhou, Y. J., Huang, M., Liu, Q., Pereira, R., David, F., & Nielsen, J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 174
- Issue
- 6
- Pages
- 1549-1558.e14
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2886850841
- Cited by
- 341
- References
- 41
- FWCI
- 11.46
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yu, T., Zhou, Y. J., Huang, M., Liu, Q., Pereira, R., David, F., & Nielsen, J. (2018). Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis. Cell. https://doi.org/10.1016/j.cell.2018.07.013
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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