Fatty acid production in genetically modified cyanobacteria

Liu, Xinyao; Sheng, Jie; Curtiss III, Roy

doi:10.1073/pnas.1103014108

Cited by 532Microbial Metabolic Engineering and BioproductionAlgal biology and biofuel productionLipid metabolism and biosynthesis

Fatty acid production in genetically modified cyanobacteria

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Liu, Xinyao, Sheng, Jie, Curtiss III, Roy·2011·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1103014108PMC PMC3084101

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Abstract

To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl–acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased to 197 ± 14 mg/L of culture in one improved strain at a cell density of 1.0 × 10 9 cells/mL by adding codon-optimized thioesterase genes and weakening polar cell wall layers. Although these strains exhibited damaged cell membranes at low cell densities, they grew more rapidly at high cell densities in late exponential and stationary phase and exhibited less cell damage than cells in wild-type cultures. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production.

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Engineering microbes to produce and excrete FFAs directly into the growth medium was recently demonstrated in the cyanobacteria Synechocystis sp. PCC6803.
“This approach was recently demonstrated in the cyanobacteria Synechocystis sp. PCC6803 (45).”
From Catalytic Hydroprocessing of Single-Cell Oils to Hydrocarbon Fuels: Converting microbial lipids to fuels is a promising approach to replace fossil fuels (2021) · cites this paper as [45]

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Authors: Liu, X., Sheng, J., & Curtiss III, R.
Affiliations: Arizona State University (US)
Center for Infectious Diseases and Vaccinology, and
ORCID: Liu, Xinyao
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2011-04-11
Volume: 108
Issue: 17
Pages: 6899-6904
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1103014108
PMC: PMC3084101
OpenAlex: W2064590397
Cited by: 532
References: 45
Citations by year: 2026: 15 · 2025: 21 · 2024: 12 · 2023: 19 · 2022: 28 · 2021: 39 · 2020: 37 · 2019: 34 · 2018: 42 · 2017: 37 · 2016: 50 · 2015: 46 · 2014: 45 · 2013: 55 · 2012: 41
FWCI: 19.59
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: fatty acid production, cyanobacteria, Synechocystis sp. PCC 6803, fatty acid secretion, codon optimization, cell wall weakening, cell membrane damage, cell density
MeSH terms: Fatty Acids, Lysophospholipase, Thiolester Hydrolases, Escherichia coli Proteins, Organisms, Genetically Modified, Periplasmic Proteins, Synechocystis, Biofuels

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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Fatty acid production in genetically modified cyanobacteria

Abstract

Integrity signals

Claims the paper makes

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Cites

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