Cited by 147Bacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsCell death mechanisms and regulation

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Magnesium Flux Modulates Ribosomes to Increase Bacterial Survival

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Lee, Dong-yeon D., Galera-Laporta, Leticia, Bialecka-Fornal, Maja, Moon, Eun Chae, Shen, Zhouxin, Briggs, Steven P., Garcia-Ojalvo, Jordi, Süel, Gürol M.·2019·Cell

DOI 10.1016/j.cell.2019.01.042PMC PMC6814349

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Abstract

Bacteria exhibit cell-to-cell variability in their resilience to stress, for example, following antibiotic exposure. Higher resilience is typically ascribed to "dormant" non-growing cellular states. Here, by measuring membrane potential dynamics of Bacillus subtilis cells, we show that actively growing bacteria can cope with ribosome-targeting antibiotics through an alternative mechanism based on ion flux modulation. Specifically, we observed two types of cellular behavior: growth-defective cells exhibited a mathematically predicted transient increase in membrane potential (hyperpolarization), followed by cell death, whereas growing cells lacked hyperpolarization events and showed elevated survival. Using structural perturbations of the ribosome and proteomic analysis, we uncovered that stress resilience arises from magnesium influx, which prevents hyperpolarization. Thus, ion flux modulation provides a distinct mechanism to cope with ribosomal stress. These results suggest new approaches to increase the effectiveness of ribosome-targeting antibiotics and reveal an intriguing connection between ribosomes and the membrane potential, two fundamental properties of cells.

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Authors: Lee, D.-Y. D., Galera-Laporta, L., Bialecka-Fornal, M., Moon, E. C., Shen, Z., Briggs, S. P., Garcia-Ojalvo, J., & Süel, G. M.
Affiliations: University of California San Diego (US)
Universitat Pompeu Fabra (ES)
Corresponding author: Süel, Gürol M.
ORCID: Lee, Dong-yeon D.
Galera-Laporta, Leticia
Moon, Eun Chae
Shen, Zhouxin
Briggs, Steven P.
Süel, Gürol M.
Venue: Cell
Publisher: Elsevier BV
Published: 2019-04
Volume: 177
Issue: 2
Pages: 352-360.e13
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/j.cell.2019.01.042
PMC: PMC6814349
OpenAlex: W2922356758
Cited by: 147
References: 46
Citations by year: 2026: 27 · 2025: 24 · 2024: 20 · 2023: 15 · 2022: 24 · 2021: 23 · 2020: 7 · 2019: 7
FWCI: 9.18
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 369 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Repositori digital de la UPF (Universitat Pompeu Fabra) (acceptedVersion)
RECERCAT (Consorci de Serveis Universitaris de Catalunya) (submittedVersion)
Funders: Spanish Ministry of Economy and Competitiveness
FEDER (FIS2015-66503-C3-1-P)
ICREA
Maria de Maeztu Program for Units of Excellence in Research and Development (MDM-2014-0370)
San Diego Center for Systems Biology
NIH (P50 GM085764)
National Institute of General Medical Sciences (R01 GM121888)
Howard Hughes Medical Institute
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords: magnesium influx, ribosome-targeting antibiotics, membrane hyperpolarization, Bacillus subtilis, cell-to-cell variability, antibiotic tolerance
MeSH terms: Bacterial Outer Membrane, Bacillus subtilis, Bacterial Proteins, Magnesium, Ribosomal Proteins, Ribosomes, Proteomics

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Magnesium Flux Modulates Ribosomes to Increase Bacterial Survival

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