The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation
Open accessAbstract
Summary Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of ten exRNA isolation methods across five biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNAseq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle- (EV), ribonucleoprotein-(RNP)-, and high-density lipoprotein- (HDL) specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provides comparative statistics for all expressed miRNAs or a selected subset of miRNAs in the desired biofluid for each exRNA isolation method, and returns a ranked list of exRNA isolation methods prioritized by complexity, expression level and reproducibility. These results will improve reproducibility and stimulate further progress in exRNA biomarker development.
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Cutadapt removes adapter sequences from high-throughput sequencing reads
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Diverse human extracellular RNAs are widely detected in human plasma
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RNA delivery by extracellular vesicles in mammalian cells and its applications
O’Brien, K., Breyne, K., Ughetto, S., Laurent, L. C., & Breakefield, X. O. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0251-y · Cited by 1,811
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Metadata
- Authors
- Srinivasan, S., Yeri, A., Cheah, P. S., Chung, A., Danielson, K., De Hoff, P., Filant, J., Laurent, C. D., Laurent, L. D., Magee, R., Moeller, C., Murthy, V. L., Nejad, P., Paul, A., Rigoutsos, I., Rodosthenous, R., Shah, R. V., Simonson, B., To, C., Wong, D., Yan, I. K., Zhang, X., Balaj, L., Breakefield, X. O., Daaboul, G., Gandhi, R., Lapidus, J., Londin, E., Patel, T., Raffai, R. L., Sood, A. K., Alexander, R. P., Das, S., & Laurent, L. C.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-04
- Volume
- 177
- Issue
- 2
- Pages
- 446-462.e16
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2931788014
- Cited by
- 304
- References
- 5
- FWCI
- 11.74
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Srinivasan, S., Yeri, A., Cheah, P. S., Chung, A., Danielson, K., De Hoff, P., Filant, J., Laurent, C. D., Laurent, L. D., Magee, R., Moeller, C., Murthy, V. L., Nejad, P., Paul, A., Rigoutsos, I., Rodosthenous, R., Shah, R. V., Simonson, B., To, C., . . . Laurent, L. C. (2019). Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell. https://doi.org/10.1016/j.cell.2019.03.024
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