The DOI matches its Crossref record; 87 of the 88 references it cites were found in a registry or an index.
Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging
Open accessAbstract
Aging causes a functional decline in tissues throughout the body that may be delayed by caloric restriction (CR). However, the cellular profiles and signatures of aging, as well as those ameliorated by CR, remain unclear. Here, we built comprehensive single-cell and single-nucleus transcriptomic atlases across various rat tissues undergoing aging and CR. CR attenuated aging-related changes in cell type composition, gene expression, and core transcriptional regulatory networks. Immune cells were increased during aging, and CR favorably reversed the aging-disturbed immune ecosystem. Computational prediction revealed that the abnormal cell-cell communication patterns observed during aging, including the excessive proinflammatory ligand-receptor interplay, were reversed by CR. Our work provides multi-tissue single-cell transcriptional landscapes associated with aging and CR in a mammal, enhances our understanding of the robustness of CR as a geroprotective intervention, and uncovers how metabolic intervention can act upon the immune system to modify the process of aging.
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In the citing paper
“However, CR has diverse effects across multiple biological systems42,43.”
From Effect of long-term caloric restriction on DNA methylation measures of biological aging in healthy adults from the CALERIE trial (2023) · cites this paper as [42]
In the citing paper
“Notably, multiple tissues exhibited an increase in the numbers and percentages of pro-inflammatory immune cells as a function of age, a conclusion independently drawn in single cell transcriptomic surveys of 7 tissues in aging rats and 3 tissues in aging mice.50,51”
From Asynchronous, contagious and digital aging (2021) · cites this paper as [50]
In the citing paper
“Aging is associated with changes in the population of immune cells infiltrates,51 and caloric restriction can reverse those trends.50”
From Asynchronous, contagious and digital aging (2021) · cites this paper as [50]
In the citing paper
“Dietary restriction can generally ‘reprogramme’ both the transcriptome and the proteome in ageing rats and mice210,211.”
From Ageing and rejuvenation of tissue stem cells and their niches (2023) · cites this paper as [210]
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Self-citationEpigenetic regulation of aging: implications for interventions of aging and diseases
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Metadata
- Authors
- Ma, S., Sun, S., Geng, L., Song, M., Wang, W., Ye, Y., Ji, Q., Zou, Z., Wang, S., He, X., Li, W., Esteban, C. R., Long, X., Guo, G., Chan, P., Zhou, Q., Belmonte, J. C. I., Zhang, W., Qu, J., & Liu, G.-H.
- Affiliations
- Chinese Academy of Sciences (CN)
- Capital Medical University (CN)
- Peking Union Medical College Hospital (CN)
- Zhejiang University (CN)
- Corresponding author
- Zhang, Weiqi
- Qu, Jing
- Liu, Guang-Hui
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2020-03
- Volume
- 180
- Issue
- 5
- Pages
- 984-1001.e22
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W3007560401
- Cited by
- 349
- References
- 88
- Citations by year
- 2026: 35 · 2025: 60 · 2024: 51 · 2023: 64 · 2022: 53 · 2021: 51 · 2020: 35
- FWCI
- 16.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China Stem Cell and Translational Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Keywords
- caloric restriction, aging, single-cell transcriptomics, single-nucleus transcriptomics, Rattus norvegicus, cell type composition, immune cells, pro-inflammatory signaling, intercellular communication, transcriptional regulatory networks, geroprotective interventions, tissue aging
- MeSH terms
- Aging, Animals, Gene Expression Regulation, Humans, Immune System, Caloric Restriction, Rats, Gene Regulatory Networks, Single-Cell Analysis, Transcriptome, Cellular Reprogramming
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