The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
FSH stimulates IRS-2 expression in human granulosa cells through cAMP/SP1, an inoperative FSH action in PCOS patients
Abstract
Follicle stimulating hormone (FSH) plays a central role in growth and differentiation of ovarian follicles. A plethora of information exists on molecular aspects of FSH responses but little is known about the mechanisms involved in its cross-talk with insulin/IGF-1 pathways implicated in the coordination of energy homeostasis in preovulatory granulosa cells (GCs). In this study, we hypothesized that FSH may regulate IRS-2 expression and thereby maintain the energy balance in GCs. We demonstrate here that FSH specifically increases IRS-2 expression in human and rat GCs. FSH-stimulated IRS-2 expression was inhibited by actinomycin D or cycloheximide. Furthermore, FSH decreases IRS-2 mRNA degradation indicating post-transcriptional stabilization. Herein, we demonstrate a role of cAMP pathway in the activation of IRS-2 expression by FSH. Scan and activity analysis of IRS-2 promoter demonstrated that FSH regulates IRS-2 expression through SP1 binding sites. FSH stimulates SP1 translocation into nucleus and its binding to IRS-2 promoter. These results are corroborated by the fact that siRNA mediated knockdown of IRS-2 decreased the FSH-stimulated PI3K activity, p-Akt levels, GLUT4 translocation and glucose uptake. However, FSH was not able to increase IRS-2 expression in GCs from PCOS women undergoing IVF. Interestingly, IRS-2 mRNA expression was downregulated in GCs from the PCOS rat model. Taken together, our findings establish that FSH induces IRS-2 expression and thereby activa…
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“In addition, the “Translocation of SLC2A4 (GLUT4) to the plasma membrane” targeted by hsa-mir-548ba in both CGC and MGC, as well as “Signalling by ERBB4” targeted by hsa-miR-548b-5p in MGC, demonstrate the importance of the corresponding miRNAs in ovarian functions [39, 40].”
From Hsa-mir-548 family expression in human reproductive tissues (2021) · cites this paper as [39]
In the citing paper
“Therefore, normal glycose metabolism is important for granulosa cell function [39].”
From Hsa-mir-548 family expression in human reproductive tissues (2021) · cites this paper as [39]
In the citing paper
“Granulosa cells of polycystic ovarian syndrome patients have a tendency to display abnormal glycose metabolism.”
From Hsa-mir-548 family expression in human reproductive tissues (2021) · cites this paper as [39]
In the citing paper
“GLUT4 is involved in glycose uptake and FSH stimulates this process in granulosa cells [39].”
From Hsa-mir-548 family expression in human reproductive tissues (2021) · cites this paper as [39]
Citations
Cites
1 retracted
Complementary roles of IRS-1 and IRS-2 in the hepatic regulation of metabolism
Taniguchi, C. M., Ueki, K., & Kahn, C. R. (2005) · Journal of Clinical Investigation · doi:10.1172/jci23187 · Cited by 272
RetractedMatInspector and beyond: promoter analysis based on transcription factor binding sites
Cartharius, K., Frech, K., Grote, K., Klocke, B., Haltmeier, M., Klingenhoff, A., Frisch, M., Bayerlein, M., & Werner, T. (2005) · Computer applications in the biosciences · doi:10.1093/bioinformatics/bti473 · Cited by 2,003
Disruption of IRS-2 causes type 2 diabetes in mice
Withers, D. J., Gutierrez, J. S., Towery, H. H., Burks, D. J., Ren, J., Previs, S. F., Zhang, Y., Bernal, D., Pons, S., Shulman, G. I., et al. (1998) · Nature · doi:10.1038/36116 · Cited by 1,783
Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility
Kumar, T. R., Wang, Y., Lu, N., & Matzuk, M. M. (1997) · Nature Genetics · doi:10.1038/ng0297-201 · Cited by 1,353
The Sp-family of transcription factors
Suske, G. (1999) · Gene · doi:10.1016/s0378-1119(99)00357-1 · Cited by 1,107
IRS proteins and the common path to diabetes
White, M. F. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00514.2001 · Cited by 925
Role of IRS-2 in insulin and cytokine signalling
Sun, X. J., Wang, L.-M., Zhang, Y., Yenush, L., Myers Jr, M. G., Glasheen, E., Lane, W. S., Pierce, J. H., & White, M. F. (1995) · Nature · doi:10.1038/377173a0 · Cited by 848
Dierich, A., Sairam, M. R., Monaco, L., Fimia, G. M., Gansmüller, A., LeMeur, M., & Sassone‐Corsi, P. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.23.13612 · Cited by 824
Trainee Forum—Writing Winning Grants.
Taymans, S. E., Moss, S. B., & Hunnicutt, G. R. (2012) · Biology of Reproduction · doi:10.1093/biolreprod/87.s1.1 · Cited by 643
Follicle dynamics and anovulation in polycystic ovary syndrome
Franks, S., Stark, J., & Hardy, K. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmn015 · Cited by 581
Research works printed but not linked: 4
- Richards, 2002, Endocrinology, 14, 1283
- Gonzalez-Robayna, 2000, Mol. Endocrinol., 14, 1283, 10.1210/mend.14.8.0500 · DOI · Paper page
- Jakimiuk, 2001, Clin. Endocrinol. Metab., 86, 1318
- Stilley, 2014, Biol. Reprod., 91, 1
Cited by
Hsa-mir-548 family expression in human reproductive tissues
Rooda, I., Kaselt, B., Liivrand, M., Smolander, O.-P., Salumets, A., & Velthut-Meikas, A. (2021) · BMC Genomic Data · doi:10.1186/s12863-021-00997-w · Cited by 17
Metadata
- Authors
- Anjali, G., Kaur, S., Lakra, R., Taneja, J., Kalsey, G. S., Nagendra, A., Shrivastav, T. G., Gouri Devi, M., Malhotra, N., Kriplani, A., & Singh, R.
- Venue
- Cellular Signalling
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 27
- Issue
- 12
- Pages
- 2452-2466
- ISSN
- 0898-6568
- Cited by
- 42
- References
- 56
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Funders
- Department of Biotechnology (DBT) (BT/PR 5379/MED/14/631/2004, BT/PR 8330/AAQ/01/313/2006)
- Council of Scientific and Industrial Research (CSIR) (37/1217/05-EMRII/2009)
- Delhi University
- Central Instrumentation Facility (CIF) (Dean(R)/R&D/2011/423)
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- The paper
- Anjali, G., Kaur, S., Lakra, R., Taneja, J., Kalsey, G. S., Nagendra, A., Shrivastav, T. G., Gouri Devi, M., Malhotra, N., Kriplani, A., & Singh, R. (2015). FSH stimulates IRS-2 expression in human granulosa cells through cAMP/SP1, an inoperative FSH action in PCOS patients. Cellular Signalling. https://doi.org/10.1016/j.cellsig.2015.09.011
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
