RGM Regulates BMP-Mediated Secondary Axis Formation in the Sea Anemone Nematostella vectensis
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Patterning of the metazoan dorsoventral axis is mediated by a complex interplay of BMP signaling regulators. Repulsive guidance molecule (RGM) is a conserved BMP coreceptor that has not been implicated in axis specification. We show that NvRGM is a key positive regulator of BMP signaling during secondary axis establishment in the cnidarian Nematostella vectensis. NvRGM regulates first the generation and later the shape of a BMP-dependent Smad1/5/8 gradient with peak activity on the side opposite the NvBMP/NvRGM/NvChordin expression domain. Full knockdown of Smad1/5/8 signaling blocks the formation of endodermal structures, the mesenteries, and the establishment of bilateral symmetry, while altering the gradient through partial NvRGM or NvBMP knockdown shifts the boundaries of asymmetric gene expression and the positioning of the mesenteries along the secondary axis. These findings provide insight into the diversification of axis specification mechanisms and identify a previously unrecognized role for RGM in BMP-mediated axial patterning.
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The expression patterns of candidate guidance molecules such as Netrin or RGM do not obviously relate to the observed changes in neurite projections of Elav1-positive neurons.
“In fact, the expression patterns of candidate guidance molecules, such as Netrin or RGM, do not obviously relate to the observed changes in neurite projections of Elav1-positive neurons [24,39].”
From Evolution of eumetazoan nervous systems: insights from cnidarians (2015) · cites this paper as [39]
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Evolution of eumetazoan nervous systems: insights from cnidarians
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- Authors
- Leclère, L., & Rentzsch, F.
- Affiliations
- University of Bergen (NO)
- Corresponding author
- Rentzsch, Fabian
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-12
- Volume
- 9
- Issue
- 5
- Pages
- 1921-1930
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W1989203963
- Cited by
- 67
- References
- 37
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 3 · 2022: 7 · 2021: 3 · 2020: 2 · 2019: 8 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 7
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Keywords
- repulsive guidance molecules, BMP signaling, Nematostella vectensis, chordin, mesentery, endoderm development, bilateral symmetry, axis specification, cnidarian development
- MeSH terms
- Animals, Sea Anemones, Signal Transduction, Gene Expression, Gene Expression Regulation, Developmental, Bone Morphogenetic Proteins, Body Patterning, Embryonic Development, Smad Proteins, Bone Morphogenetic Protein Receptors, Gastrulation
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