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Cited by 108Neurobiology and Insect Physiology ResearchMemory and Neural MechanismsNeuroscience and Neuropharmacology Research

Cell-Type-Specific Transcriptome Analysis in the Drosophila Mushroom Body Reveals Memory-Related Changes in Gene Expression

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Crocker, Amanda, Guan, Xiao-Juan, Murphy, Coleen T., Murthy, Mala·2016·Cell Reports
DOI 10.1016/j.celrep.2016.04.046PMC PMC5047377
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Abstract

Learning and memory formation in Drosophila rely on a network of neurons in the mushroom bodies (MBs). Whereas numerous studies have delineated roles for individual cell types within this network in aspects of learning or memory, whether or not these cells can also be distinguished by the genes they express remains unresolved. In addition, the changes in gene expression that accompany long-term memory formation within the MBs have not yet been studied by neuron type. Here, we address both issues by performing RNA sequencing on single cell types (harvested via patch pipets) within the MB. We discover that the expression of genes that encode cell surface receptors is sufficient to identify cell types and that a subset of these genes, required for sensory transduction in peripheral sensory neurons, is not only expressed within individual neurons of the MB in the central brain, but is also critical for memory formation.

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  • Co-transmission of a fast-acting transmitter and a neuropeptide was more common.

    “Co-transmission of a fast-acting transmitter and a neuropeptide was more common, e.g., Kenyon cells (at least acetylcholine, sparkly and short neuropeptide F),21,25,55,164 the dorsal paired medial (DPM) neuron (GABA, serotonin, sparkly, nplp1, proctolin, orcokinin, eclosion hormone, CG34136)25,56,60 and most central complex, mushroom body output and mushroom body dopaminergic neurons.25,157”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [164]

    The Results (Cell Surface Receptor Gene Expression in KCs and MB extrinsic neurons; page unavailable) identify Kenyon cells as cholinergic and report high sNPF expression in two Kenyon-cell classes. They also report neurotransmitter and peptide-gene expression in sampled mushroom-body output neurons. These findings support examples of cells with both transmitter and peptide markers, but do not establish actual co-transmission, that it was more common, or the sentence’s claims about DPM, central-complex, and dopaminergic neurons. (LLM, conf=0.83)

    Experimental — accuracy not yet measured

  • Immunohistochemistry and RNA sequencing data have shown that Kenyon cells express the machinery necessary for cholinergic transmission.

    “Immunohistochemistry and RNA sequencing data have shown that Kenyon cells express the machinery necessary for cholinergic transmission.21,55,164”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [164]

    The Results section (‘Cell Surface Receptor Gene Expression in KCs and MB extrinsic neurons’; page not provided) says the authors’ analysis found Kenyon cells to be cholinergic, supporting the RNA-sequencing part of the claim. The cited passages do not show immunohistochemical evidence for cholinergic machinery, so that part is not supported by this paper’s copy. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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Authors
Crocker, A., Guan, X.-J., Murphy, C. T., & Murthy, M.
Affiliations
Princeton University (US)
Corresponding author
Murthy, Mala
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2016-05
Volume
15
Issue
7
Pages
1580-1596
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2016.04.046
PMC
PMC5047377
OpenAlex
W2345520071
Cited by
108
References
6
Citations by year
2026: 1 · 2025: 8 · 2024: 17 · 2023: 7 · 2022: 5 · 2021: 15 · 2020: 17 · 2019: 12 · 2018: 13 · 2017: 11 · 2016: 2
FWCI
7.13
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Alfred P. Sloan Foundation
  • McKnight Endowment Fund for Neuroscience
  • Israel National Road Safety Authority
  • Klingenstein Third Generation Foundation
  • National Institutes of Health
  • National Science Foundation
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
mushroom body, Drosophila, learning and memory, long-term memory formation, cell type-specific gene expression, single-cell transcriptomics, cell surface receptors
MeSH terms
Animals, Drosophila melanogaster, Gene Expression Regulation, Memory, Neurons, Receptors, Cell Surface, RNA, Messenger, Reproducibility of Results, Genes, Insect, Sequence Analysis, RNA, Gene Expression Profiling, Mushroom Bodies, Gene Ontology

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