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Cited by 12Force Microscopy Techniques and ApplicationsProtein Structure and DynamicsDrug Transport and Resistance Mechanisms

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Probing the stability and interdomain interactions in the ABC transporter OpuA using single-molecule optical tweezers

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van der Sleen, Lyan, Stevens, Jan A., Marrink, Siewert J., Poolman, Bert, Tych, Kasia·2024·Cell Reports
DOI 10.1016/j.celrep.2024.114110

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Abstract

Transmembrane transporter proteins are essential for maintaining cellular homeostasis and, as such, are key drug targets. Many transmembrane transporter proteins are known to undergo large structural rearrangements during their functional cycles. Despite the wealth of detailed structural and functional data available for these systems, our understanding of their dynamics and, consequently, how they function is generally limited. We introduce an innovative approach that enables us to directly measure the dynamics and stability of interdomain interactions of transmembrane proteins using optical tweezers. Focusing on the osmoregulatory ATP-binding cassette transporter OpuA from Lactococcus lactis, we examine the mechanical properties and potential interactions of its substrate-binding domains. Our measurements are performed in lipid nanodiscs, providing a native-mimicking environment for the transmembrane protein. The technique provides high spatial and temporal resolution and allows us to study the functionally relevant motions and interdomain interactions of individual transmembrane transporter proteins in real time in a lipid bilayer.

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Metadata

Authors
van der Sleen, L., Stevens, J. A., Marrink, S. J., Poolman, B., & Tych, K.
Affiliations
University of Groningen (NL)
Corresponding author
Poolman, Bert
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2024-04
Volume
43
Issue
4
Pages
114110
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2024.114110
OpenAlex
W4394728395
Cited by
12
References
2
Citations by year
2026: 5 · 2025: 5 · 2024: 1
FWCI
1.75
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • SSRN Electronic Journal (acceptedVersion)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
NWO (OCENW.KLEIN.526)
Topics
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
OpuA, ABC transporters, domain-domain interactions, protein stability, substrate-binding domain, osmoregulation, Lactococcus lactis, lipid nanodiscs, single-molecule force spectroscopy, optical tweezers, protein mechanical properties
MeSH terms
Protein Domains, Single Molecule Imaging, Bacterial Proteins, Lipid Bilayers, Protein Binding, Lactococcus lactis, ATP-Binding Cassette Transporters, Optical Tweezers, Protein Stability

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