Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells
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Abstract
RNA-targeting CRISPR-Cas13 proteins have recently emerged as a powerful platform to modulate gene expression outcomes. However, protein and CRISPR RNA (crRNA) delivery in human cells can be challenging with rapid crRNA degradation yielding transient knockdown. Here we compare several chemical RNA modifications at different positions to identify synthetic crRNAs that improve RNA targeting efficiency and half-life in human cells. We show that co-delivery of modified crRNAs and recombinant Cas13 enzyme in ribonucleoprotein (RNP) complexes can alter gene expression in primary CD4+ and CD8+ T cells. This system represents a robust and efficient method to modulate transcripts without genetic manipulation.
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crRNAs are typically protected from degradation while complexed with Cas13, but the ends of longer bcgRNA molecules may still be accessible and susceptible to degradation.
“While we expect that crRNA are typically protected from degradation while complexed with Cas13 (refs. 31,32), the ends of longer bcgRNA molecules may still be accessible and susceptible to degradation.”
From Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq (2023) · cites this paper as [31]
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Cites
Cancer immunotherapy using checkpoint blockade
Ribas, A., & Wolchok, J. D. (2018) · Science · doi:10.1126/science.aar4060 · Cited by 6,791 · see citing works
Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V. K., Donghia, N. M., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,887 · see citing works
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Gootenberg, J. S., Abudayyeh, O. O., Kellner, M. J., Joung, J., Collins, J. J., & Zhang, F. (2018) · Science · doi:10.1126/science.aaq0179 · Cited by 2,662 · see citing works
D’Hulst, G., Soro-Arnaiz, I., Masschelein, E., Veys, K., Fitzgerald, G., Smeuninx, B., Kim, S., Deldicque, L., Blaauw, B., Carmeliet, P., et al. (2020) · Nature Communications · doi:10.1038/s41467-019-13889-6 · Cited by 2,310 · see citing works
The Architecture of SARS-CoV-2 Transcriptome
Kim, D., Lee, J.-Y., Yang, J.-S., Kim, J. W., Kim, V. N., & Chang, H. (2020) · Cell · doi:10.1016/j.cell.2020.04.011 · Cited by 2,123 · see citing works
RNA targeting with CRISPR–Cas13
Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T., Kellner, M. J., Regev, A., et al. (2017) · Nature · doi:10.1038/nature24049 · Cited by 2,093 · see citing works
No evidence that carotenoid pigments boost either immune or antioxidant defenses in a songbird
Koch, R. E., Kavazis, A. N., Hasselquist, D., Hood, W. R., Zhang, Y., Toomey, M. B., & Hill, G. E. (2018) · Nature Communications · doi:10.1038/s41467-018-02974-x · Cited by 1,924 · see citing works
Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017) · Science · doi:10.1126/science.aaq0180 · Cited by 1,806 · see citing works
Pipkins, H. R., Bradshaw, J. L., Keller, L. E., Swiatlo, E., & McDaniel, L. S. (2017) · PLOS ONE · doi:10.1371/journal.pone.0179159 · Cited by 1,338 · see citing works
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Konermann, S., Lotfy, P., Brideau, N. J., Oki, J., Shokhirev, M. N., & Hsu, P. D. (2018) · Cell · doi:10.1016/j.cell.2018.02.033 · Cited by 1,164 · see citing works
First-Line Lorlatinib or Crizotinib in Advanced ALK -Positive Lung Cancer
Shaw, A. T., Bauer, T. M., de Marinis, F., Felip, E., Goto, Y., Liu, G., Mazières, J., Kim, D., Mok, T. S. K., Polli, A., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2027187 · Cited by 1,125 · see citing works
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Hendel, A., Bak, R. O., Clark, J. T., Kennedy, A. B., Ryan, D. E., Roy, S., Steinfeld, I., Lunstad, B. D., Kaiser, R. J., Wilkens, A. B., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3290 · Cited by 1,090 · see citing works
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
Fozouni, P., Son, S., Derby, M. D. D. L., Knott, G. J., Gray, C. N., D’Ambrosio, M. V., Zhao, C., Switz, N. A., Kumar, G. R., Stephens, S. I., et al. (2020) · Cell · doi:10.1016/j.cell.2020.12.001 · Cited by 1,060 · see citing works
Shemer, A., Grozovski, J., Tay, T. L., Tao, J., Volaski, A., Süß, P., Ardura-Fabregat, A., Gross-Vered, M., Kim, J.-S., David, E., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07548-5 · Cited by 1,052 · see citing works
In situ programming of leukaemia-specific T cells using synthetic DNA nanocarriers
Smith, T. T., Stephan, S. B., Moffett, H. F., McKnight, L. E., Ji, W., Reiman, D., Bonagofski, E., Wohlfahrt, M. E., Pillai, S. P. S., & Stephan, M. T. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2017.57 · Cited by 788 · see citing works
Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
Joung, J., Ladha, A., Saito, M., Kim, N.-G., Woolley, A. E., Segel, M., Barretto, R. P. J., Ranu, A., Macrae, R. K., Faure, G., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmc2026172 · Cited by 780 · see citing works
Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
Patchsung, M., Jantarug, K., Pattama, A., Aphicho, K., Suraritdechachai, S., Meesawat, P., Sappakhaw, K., Leelahakorn, N., Ruenkam, T., Wongsatit, T., et al. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-020-00603-x · Cited by 642 · see citing works
Churchill, A., Gutiérrez, G. D., Singer, R. A., Lorberbaum, D. S., Fischer, K. A., & Sussel, L. (2017) · eLife · doi:10.7554/elife.20010 · Cited by 543 · see citing works
Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
Arizti-Sanz, J., Freije, C. A., Stanton, A. C., Petros, B. A., Boehm, C. K., Siddiqui, S. M., Shaw, B. M., Adams, G. C., Kosoko-Thoroddsen, T.-S. F., Kemball, M. E., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19097-x · Cited by 513 · see citing works
Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza
Abbott, T. R., Dhamdhere, G. R., Liu, Y., Lin, X., Goudy, L., Zeng, L., Chemparathy, A. G., Chmura, S. A., Heaton, N. S., Debs, R. J., et al. (2020) · Cell · doi:10.1016/j.cell.2020.04.020 · Cited by 500 · see citing works
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
Yin, H., Song, C.-Q., Suresh, S., Wu, Q., Walsh, S., Rhym, L. H., Mintzer, E., Bolukbasi, M. F., Zhu, L. J., Kauffman, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4005 · Cited by 499 · see citing works
Hu, F., Jiang, X. W., Guo, C., Li, Y., Chen, S., Zhang, W., Du, Y., Wang, P., Zheng, X., Fang, X., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15700-3 · Cited by 419 · see citing works
Massively parallel Cas13 screens reveal principles for guide RNA design
Wessels, H.-H., Méndez-Mancilla, A., Guo, X., Legut, M., Daniloski, Z., & Sanjana, N. E. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0456-9 · Cited by 418 · see citing works
Self-citationProgrammable Inhibition and Detection of RNA Viruses Using Cas13
Freije, C. A., Myhrvold, C., Boehm, C. K., Lin, A. E., Welch, N. L., Carter, A., Metsky, H. C., Luo, C. Y., Abudayyeh, O. O., Gootenberg, J. S., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.09.013 · Cited by 389 · see citing works
A population genetic interpretation of GWAS findings for human quantitative traits
Simons, Y. B., Bullaughey, K., Hudson, R. R., & Sella, G. (2018) · PLoS Biology · doi:10.1371/journal.pbio.2002985 · Cited by 363 · see citing works
Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems
Yang, L.-Z., Wang, Y., Li, S., Yao, R.-W., Luan, P.-F., Wu, H., Carmichael, G., & Chen, L. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.10.024 · Cited by 350 · see citing works
CRISPR-mediated live imaging of genome editing and transcription
Wang, H., Nakamura, M., Abbott, T. R., Zhao, D., Luo, K., Yu, C., Nguyen, C. M., Lo, A., Daley, T., Russa, M. F. L., et al. (2019) · Science · doi:10.1126/science.aax7852 · Cited by 292 · see citing works
Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs
Ryan, D. E., Taussig, D., Steinfeld, I., Phadnis, S. M., Lunstad, B. D., Singh, M., Vuong, X., Okochi, K. D., McCaffrey, R., Olesiak, M., et al. (2018) · Nucleic Acids Research · doi:10.1093/nar/gkx1199 · Cited by 280 · see citing works
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d
Zhang, C., Konermann, S., Brideau, N. J., Lotfy, P., Wu, X., Novick, S. J., Strutzenberg, T., Griffin, P. R., Hsu, P. D., & Lyumkis, D. (2018) · Cell · doi:10.1016/j.cell.2018.09.001 · Cited by 276 · see citing works
Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein
Li, J., Chen, Z., Chen, F., Xie, G., Ling, Y., Peng, Y., Yu, L., Luo, N., Chiang, C., & Wang, H. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa269 · Cited by 238 · see citing works
CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos
Kushawah, G., Hernández-Huertas, L., del Prado, J. A.-N., Martínez‐Morales, J. R., DeVore, M. L., Hassan, H., Moreno-Sánchez, I., Tomás‐Gallardo, L., Díaz‐Moscoso, A., Monges, D. E., et al. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.07.013 · Cited by 227 · see citing works
Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents
Blanchard, E. L., Vanover, D., Bawage, S., Tiwari, P., Rotolo, L., Beyersdorf, J. P., Peck, H., Bruno, N. C., Hincapie, R., Michel, F., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00822-w · Cited by 212 · see citing works
Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
Cromwell, C. R., Sung, K., Park, J., Krysler, A. R., Jovel, J., Kim, S. K., & Hubbard, B. P. (2018) · Nature Communications · doi:10.1038/s41467-018-03927-0 · Cited by 190 · see citing works
Barnes, K. G., Lachenauer, A. E., Nitido, A. D., Siddiqui, S. M., Gross, R., Beitzel, B. F., Siddle, K. J., Freije, C. A., Dighero-Kemp, B., Mehta, S. B., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-17994-9 · Cited by 181 · see citing works
Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering
Lee, K., Mackley, V. A., Rao, A., Chong, A. T., DeWitt, M. A., Corn, J. E., & Murthy, N. (2017) · eLife · doi:10.7554/elife.25312 · Cited by 141 · see citing works
Transcription factor TFCP2L1 patterns cells in the mouse kidney collecting ducts
Werth, M., Schmidt-Ott, K. M., Leete, T., Qiu, A., Hinze, C., Viltard, M., Paragas, N., Shawber, C. J., Yu, W., Lee, P., et al. (2017) · eLife · doi:10.7554/elife.24265 · Cited by 122 · see citing works
In vitro–transcribed guide RNAs trigger an innate immune response via the RIG-I pathway
Wienert, B., Shin, J. J., Zelin, E., Pestal, K., & Corn, J. E. (2018) · PLoS Biology · doi:10.1371/journal.pbio.2005840 · Cited by 117 · see citing works
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Jiang, T., Henderson, J. M., Coote, K. J., Cheng, Y., Valley, H. C., Zhang, X.-O., Wang, Q., Rhym, L. H., Cao, Y., Newby, G. A., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15892-8 · Cited by 110 · see citing works
Conditional control of RNA-guided nucleic acid cleavage and gene editing
Wang, S., Wu, L., Huang, H., Xiong, W., Liu, J., Wei, L., Yin, P., Tian, T., & Zhou, X. (2020) · Nature Communications · doi:10.1038/s41467-019-13765-3 · Cited by 96 · see citing works
CRISPR–Cas13 Precision Transcriptome Engineering in Cancer
Granados-Riveron, J. T., & Aquino‐Jarquín, G. (2018) · Cancer Research · doi:10.1158/0008-5472.can-18-0785 · Cited by 91 · see citing works
Structure, function, and control of the human musculoskeletal network
Murphy, A. C., Muldoon, S. F., Baker, D., Lastowka, A., Bennett, B., Yang, M., & Bassett, D. S. (2018) · PLoS Biology · doi:10.1371/journal.pbio.2002811 · Cited by 87 · see citing works
Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells
McMahon, M. A., Prakash, T. P., Cleveland, D. W., Bennett, C. F., & Rahdar, M. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.02.031 · Cited by 87 · see citing works
Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens
Guo, X., Rahman, J. A., Wessels, H.-H., Méndez-Mancilla, A., Haro, D., Chen, X., & Sanjana, N. E. (2021) · Cell Genomics · doi:10.1016/j.xgen.2021.100001 · Cited by 79 · see citing works
Self-citationBasila, M., Kelley, M. L., & Smith, A. V. B. (2017) · PLoS ONE · doi:10.1371/journal.pone.0188593 · Cited by 73 · see citing works
RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering
Smargon, A. A., Shi, Y. J., & Yeo, G. W.-M. (2020) · Nature Cell Biology · doi:10.1038/s41556-019-0454-7 · Cited by 64 · see citing works
Quinto‐Sánchez, M., Cintas, C., de Cerqueira, C. C. S., Ramallo, V., Acuña-Alonzo, V., Adhikari, K., Castillo, L. D., Gómez‐Valdés, J., Everardo, P., de Ávila, F., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0169287 · Cited by 55 · see citing works
Abrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells
Cui, J., Techakriengkrai, N., Nedumpun, T., & Suradhat, S. (2020) · Scientific Reports · doi:10.1038/s41598-020-66775-3 · Cited by 52 · see citing works
Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2Preprint
Arizti-Sanz, J., Freije, C. A., Stanton, A. C., Boehm, C. K., Petros, B. A., Siddiqui, S. M., Shaw, B. M., Adams, G. C., Kosoko-Thoroddsen, T.-S. F., Kemball, M. E., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.05.28.119131 · Cited by 44 · see citing works
Coronavirus leader‐RNA‐primed transcription: An alternative mechanism to RNA splicing
Lai, M. M. C. (1986) · BioEssays · doi:10.1002/bies.950050606 · Cited by 43 · see citing works
Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogensPreprint
Guo, X., Wessels, H., Méndez‐Mancilla, A., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.08.20.259762 · Cited by 17 · see citing works
Self-citation
Flagged first, then by citations
2 of the 52 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
CRISPR technologies for genome, epigenome and transcriptome editing
Villiger, L., Joung, J., Koblan, L. W., Weissman, J. S., Abudayyeh, O. O., & Gootenberg, J. S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00697-6 · Cited by 264 · see citing works
Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
Wessels, H., Stirn, A., Méndez‐Mancilla, A., Kim, E. J., Hart, S. K., Knowles, D. A., & Sanjana, N. E. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01830-8 · Cited by 135 · see citing works
Self-citationRNA chemistry and therapeutics
Wang, S., Weissman, D., & Dong, Y. (2025) · Nature Reviews Drug Discovery · doi:10.1038/s41573-025-01237-x · Cited by 98 · see citing works
Chemical Modifications of CRISPR RNAs to Improve Gene-Editing Activity and Specificity
Rozners, E. (2022) · Journal of the American Chemical Society · doi:10.1021/jacs.2c02633 · Cited by 85 · see citing works
Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq
Wessels, H.-H., Méndez-Mancilla, A., Hao, Y., Papalexi, E., Mauck, W. M., Lu, L., Morris, J. A., Mimitou, E. P., Smibert, P., Sanjana, N. E., et al. (2023) · Nature Methods · doi:10.1038/s41592-022-01705-x · Cited by 79 · see citing works
Self-citationTranscriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells
Liang, W.-W., Müller, S., Hart, S. K., Wessels, H., Méndez-Mancilla, A., Sookdeo, A., Choi, O., Caragine, C. M., Corman, A., Lu, L., et al. (2024) · Cell · doi:10.1016/j.cell.2024.10.021 · Cited by 62 · see citing works
Self-citationRetractedEngineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging
Cao, L., Wang, Z., Lei, C., & Nie, Z. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c06789 · Cited by 56 · see citing works
Broad-spectrum CRISPR-mediated inhibition of SARS-CoV-2 variants and endemic coronaviruses in vitro
Zeng, L., Liu, Y., Wrynla, X. H., Abbott, T. R., Han, M., Zhu, Y., Chemparathy, A. G., Lin, X., Chen, X., Wang, H., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-30546-7 · Cited by 52 · see citing works
Self-citationSun, Y., Chen, W., Liu, J., Li, J., Zhang, Y., Cai, W., Liu, L., Tang, X., Hou, J., Wang, M., et al. (2022) · Angewandte Chemie International Edition · doi:10.1002/anie.202212413 · Cited by 55 · see citing works
Unmodificated stepless regulation of CRISPR/Cas12a multi-performance
Zhao, R., Luo, W., Wu, Y., Zhang, L., Liu, X., Li, J., Yang, Y., Wang, L., Wang, L., Han, X., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad748 · Cited by 53 · see citing works
Jang, H., Song, J., Kim, S., Byun, J., Lee, K. G., Park, K., Woo, E., Lim, E., Jung, J., & Kang, T. (2023) · Nature Communications · doi:10.1038/s41467-023-43899-4 · Cited by 53 · see citing works
Chemical engineering of CRISPR–Cas systems for therapeutic application
Barber, H. M., Pater, A. A., Gagnon, K. T., Damha, M. J., & O’Reilly, D. (2024) · Nature Reviews Drug Discovery · doi:10.1038/s41573-024-01086-0 · Cited by 55 · see citing works
dCas13-mediated translational repression for accurate gene silencing in mammalian cells
Apostolopoulos, A., Kawamoto, N., Chow, S. Y. A., Tsuiji, H., Ikeuchi, Y., Shichino, Y., & Iwasaki, S. (2024) · Nature Communications · doi:10.1038/s41467-024-46412-7 · Cited by 51 · see citing works
Wan, Y., Li, S., Xu, W., Wang, K., Guo, W., Yang, C., Li, X., Zhou, J., & Wang, J. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c03698 · Cited by 44 · see citing works
Yang, Y., Sun, L., Zhao, J., Jiao, Y., Han, T., & Zhou, X. (2024) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2024.116239 · Cited by 43 · see citing works
Alariqi, M., Ramadan, M., Yu, L., Hui, F., Hussain, A., Zhou, X., Yu, Y., Zhang, X., & Jin, S. (2025) · Advanced Science · doi:10.1002/advs.202416331 · Cited by 42 · see citing works
Insights Gained from RNA Editing Targeted by the CRISPR-Cas13 Family
Liu, L., & Pei, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911400 · Cited by 39 · see citing works
Spiking neural networks for nonlinear regression
Henkes, A., Eshraghian, J. K., & Wessels, H. (2024) · Royal Society Open Science · doi:10.1098/rsos.231606 · Cited by 39 · see citing works
Self-citationRecent advances in CRISPR-based single-nucleotide fidelity diagnostics
Kohabir, K. A. V., Sistermans, E. A., & Wolthuis, R. M. F. (2025) · Communications Medicine · doi:10.1038/s43856-025-00933-4 · Cited by 39 · see citing works
Morris, J. A., Sun, J. S., & Sanjana, N. E. (2023) · Trends in Genetics · doi:10.1016/j.tig.2023.10.012 · Cited by 30 · see citing works
Self-citationEnhanced RNA-targeting CRISPR-Cas technology in zebrafish
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Goyon, A., Nguyen, D. N., Boulanouar, S., Yehl, P., & Zhang, K. (2022) · Analytical Chemistry · doi:10.1021/acs.analchem.2c04681 · Cited by 26 · see citing works
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Gunitseva, N. M., Evteeva, M. A., Borisova, A., Patrushev, M. V., & Subach, F. V. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24086894 · Cited by 26 · see citing works
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Shembrey, C., Yang, R., Casan, J. M. L., Hu, W., Chen, H., Singh, G. J., Sadras, T., Prasad, K., Shortt, J., Johnstone, R. W., et al. (2024) · Science Advances · doi:10.1126/sciadv.adl0731 · Cited by 26 · see citing works
Wang, L., Wen, X., Yang, Y., Hu, Z. M., Jiang, J., Duan, L., Liao, X., He, Y., Liu, Y., Wang, J., et al. (2024) · Communications Biology · doi:10.1038/s42003-024-06368-2 · Cited by 23 · see citing works
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Wang, L., & Han, H. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e38588 · Cited by 25 · see citing works
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Li, Y., Li, D., & Chen, L. (2023) · Genome biology · doi:10.1186/s13059-023-02848-6 · Cited by 21 · see citing works
Multiplexed engineering and precision gene editing in cellular immunotherapy
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Advances of CRISPR-Cas13 system in COVID-19 diagnosis and treatment
Zhou, Q., Chen, Y., Wang, R., Jia, F., He, F., & Yuan, F. (2022) · Genes & Diseases · doi:10.1016/j.gendis.2022.11.016 · Cited by 21 · see citing works
Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13
Burris, B. J. D., Molina Vargas, A. M., Park, B. J., & O'Connell, M. R. (2022) · Methods · doi:10.1016/j.ymeth.2022.08.007 · Cited by 13 · see citing works
Transcriptomics and RNA-Based Therapeutics as Potential Approaches to Manage SARS-CoV-2 Infection
Arriaga-Canon, C., Contreras‐Espinosa, L., Rebollar‐Vega, R., Montiel-Manríquez, R., Cedro‐Tanda, A., García-Gordillo, J. A., Álvarez-Gómez, R. M., Jiménez-Trejo, F. J., Hernández, C. C., & Herrera, L. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911058 · Cited by 14 · see citing works
Sun, Y., Chen, W., Liu, J., Li, J., Zhang, Y., Cai, W., Liu, L., Tang, X., Hou, J., Wang, M., et al. (2022) · Angewandte Chemie · doi:10.1002/ange.202212413 · Cited by 13 · see citing works
Zhao, R., Wang, C., 李佳楣, Liao, Y., Huang, C., Hu, T., Zhang, H., & Zhang, W. (2026) · Molecular Biomedicine · doi:10.1186/s43556-026-00456-x · Cited by 12 · see citing works
Steric regulation of CRISPR/Cas12a trans -cleavage kinetics via split-activator extensions
Zhang, J., He, X., Huang, J., Cheng, C., He, G., Xia, R., Yang, J., Jianmei, C., Guo, L., Xiang, D., et al. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkaf1535 · Cited by 5 · see citing works
Hernández-Huertas, L., Moreno-Sánchez, I., Crespo-Cuadrado, J., Vargas-Baco, A., Pescador, G. D. S., Zhang, Y., Wen, Z., Florens, L. A., Santos-Pereira, J. M., Bazzini, A., et al. (2025) · The EMBO Journal · doi:10.1038/s44318-025-00617-8 · Cited by 5 · see citing works
Iqbal, F. (2023) · International Journal of Health Sciences · doi:10.53730/ijhs.v7ns1.14397 · Cited by 4 · see citing works
Zhao, G., Li, J., Fu, S., Wang, A., Meng, X., Dong, H., & Zhang, X. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118639 · Cited by 3 · see citing works
Precision Transcriptome Editing
Chen, C., & Qi, L. S. (2024) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00183 · Cited by 3 · see citing works
Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos
Ge, W., Zhao, X., Gou, S., Jin, Q., Chen, F., Ouyang, Z., Lai, C., Cui, T., Mai, B., Lü, S., et al. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.102035 · Cited by 3 · see citing works
Optical control of CRISPR-Cas editing with cyclically caged guide RNAsPreprint
Sun, Y.-J., Liu, J., Li, J., Zhang, Y., Chen, W., Cai, W., Liu, L., Tang, X., Hou, J., Wang, M., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.03.04.482981 · Cited by 3 · see citing works
CRISPR as a next-generation environmental biosurveillance tool for air, land, and water
Durán‐Vinet, B., Stanton, J.-A. L., Jeunen, G., Pochon, X., Zaiko, A., & Gemmell, N. J. (2025) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.11.014 · Cited by 2 · see citing works
Chemical biology of precise RNA targeting and intervention: advances and perspectives
Wu, H., Zhuang, Y., Peng, J., & Yi, C. (2026) · Science China Chemistry · doi:10.1007/s11426-025-3018-0 · Cited by 2 · see citing works
Essential lncRNAs in the human transcriptome
Liang, W.-W., Mueller, S. J., Hart, S. K., Wessels, H., Méndez‐Mancilla, A., Sookdeo, A., Choi, O., Caragine, C. M., Corman, A., Lu, L., et al. (2026) · Cell Genomics · doi:10.1016/j.xgen.2026.101253 · Cited by 3 · see citing works
Self-citationZou, G., Liu, X., Li, Y., Li, X., Wang, J., He, X., Zhu, T., & Liu, C. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118981 · Cited by 2 · see citing works
CRISPR/Cas9 based manipulating oncogenic chromosomal changes in vivo
Iqbal, F. (2023) · International Journal of Health Sciences · doi:10.53730/ijhs.v7ns1.14461 · Cited by 2 · see citing works
A CRISPR-Cas13b System Degrades SARS-CoV and SARS-CoV-2 RNA In Vitro
Andersson, K., Azatyan, A., Ekenberg, M., Akpinar, G. G., Puig, L. S., Puumalainen, M.-R., Pramer, T., Monteil, V., & Mirazimi, A. (2024) · Viruses · doi:10.3390/v16101539 · Cited by 2 · see citing works
Dang, D. T. (2025) · 3 Biotech · doi:10.1007/s13205-025-04354-x · Cited by 1 · see citing works
Wu, Y., Zhou, S., Ye, X., Zhu, T., Deng, F., & Yang, D. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118893 · Cited by 1 · see citing works
Hou, S., Bi, W., Liu, W. T., Niê, H. N., Jiang, J., & Zeng, L. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkag616 · Cited by 1 · see citing works
Fire Burn and Cauldron Bubble: What Is in Your Genome Editing Brew?
Patange, S., & Maragh, S. (2022) · Biochemistry · doi:10.1021/acs.biochem.2c00431 · Cited by 1 · see citing works
Rahimi, K., Andersen, M. S., Seeler, S., Hansen, T. B., & Kjems, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.08.30.505797
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L. C., Li, D., & Chen, L. (2023) · Figshare · doi:10.6084/m9.figshare.22605973
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L. C., Li, D., & Chen, L. (2023) · Figshare · doi:10.6084/m9.figshare.22605979
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L. C., Li, D., & Chen, L. (2023) · Figshare · doi:10.6084/m9.figshare.22605970
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L. C., Li, D., & Chen, L. (2023) · Figshare · doi:10.6084/m9.figshare.22605976
Huang, Y., Gao, B., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L. C., Li, D., & Chen, L. (2023) · Figshare · doi:10.6084/m9.figshare.22605967
Studies on Zebrafish Hemostasis Disorders
Dhinoja, S. (2024) · doi:10.12794/metadc2415973
Genetic Engineering Methods in Primary T Cells
Youssef, A., & Li, H.-S. (2025) · Advanced Therapeutics · doi:10.1002/adtp.202500149
Huang, Y., Gao, B.-Q., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L., Li, D., & Chen, L.-L. (2023) · Figshare · doi:10.6084/m9.figshare.22605988
Huang, Y., Gao, B.-Q., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L., Li, D., & Chen, L.-L. (2023) · Figshare · doi:10.6084/m9.figshare.22605964
Huang, Y., Gao, B.-Q., Meng, Q., Yang, L.-Z., Ma, X.-K., Wu, H., Pan, Y.-H., Yang, L., Li, D., & Chen, L.-L. (2023) · Figshare · doi:10.6084/m9.figshare.22605985
Establishing a chimeric tRNA-sgRNA scaffold and computational basis for enhanced CRISPR interference
Jin, G., Yang, C., Deng, Q., Wu, L., Chen, W., & Chen, Z. (2025) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2025.153222
Performance enhancement of CRISPR-Cas system based on improved guide RNA: a review
Zhang, X., Tian, C., Wang, M., Jia, H., Li, X., & Tian, G. (2026) · Analytica Chimica Acta · doi:10.1016/j.aca.2026.345472
Siddiqui, S., Parab, S. B., & Bhatt, L. K. (2026) · Biotechnology Journal · doi:10.1002/biot.70262
Kaur, K., Chandel, S., & Singh, R. (2026) · Next Nanotechnology · doi:10.1016/j.nxnano.2026.100741
Enhanced CRISPR-Cas3-mediated genome editing using circularized crRNAs
Mikamo, K., Yoshimi, K., Abe, H., Takeshita, K., Abe, H., & Mashimo, T. (2026) · iScience · doi:10.1016/j.isci.2026.117481
Ding, W., Yang, X., Yang, Y., Liu, M., Liu, C., Wang, J., & Liu, X. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkag893
Zhakipbekov, K. S., Eshkabilova, S., Kayimovich, R. Z., Eshonov, S., Хасанов, А. А., Tokhtamurod, Z., Ashurmetov, A., Abduraxmanov, A., ogli, A. N. K., Eshqobilov, E., et al. (2026) · Clinical and Translational Discovery · doi:10.1002/ctd2.70211
Combined RNA- and DNA-targeting CRISPR ribonucleoproteins enhance suppression of HBV transcripts
Banik, P., Hill, A. C., Schank, M., Zhang, Y., Zhao, J., Pyburn, J. S., Orfield, H. K., Wu, Y., Hajipour, M. J., Gazzar, M. E., et al. (2026) · Frontiers in Genome Editing · doi:10.3389/fgeed.2026.1824895
Cas14/Cas12f biosensing: ssDNA input engineering, signal transduction, and analytical applications
Sun, K., Huang, L., Ma, G., Bao, F., Hou, L., Tang, Q., Ma, B., Wang, W., 涂鹏程, Shentu, X., et al. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.119180
Moovendhan, M. (2026) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2026.109068
- Authors
- Méndez-Mancilla, A., Wessels, H.-H., Legut, M., Kadina, A., Mabuchi, M., Walker, J., Robb, G. B., Holden, K., & Sanjana, N. E.
- Affiliations
- New York Genome Center (US)
- Synthego (United States) (US)
- New England Biolabs (United States) (US)
- Corresponding author
- Sanjana, Neville E.
- Venue
- Cell Chemical Biology
- Publisher
- Elsevier BV
- Published
- 2022-02
- Volume
- 29
- Issue
- 2
- Pages
- 321-327.e4
- ISSN
- 2451-9448 (ISSN-L)
- 2451-9456
- PMC
- PMC8792099
- OpenAlex
- W3190407283
- Cited by
- 68
- References
- 52
- Citations by year
- 2026: 16 · 2025: 11 · 2024: 14 · 2023: 13 · 2022: 14
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 382 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell chemical biology (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Human Genome Research Institute (DP2HG010099)
- New York University
- National Cancer Institute (R01CA218668)
- New York Genome Center
- National Institute of General Medical Sciences (R01GM138635)
- Defense Advanced Research Projects Agency (D18AP00053)
- National Institutes of Health
- Cancer Research Institute
- Brain and Behavior Research Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- CRISPR-Cas13, ribonucleoprotein complexes, gene knockdown, T cells
- MeSH terms
- Gene Editing, RNA, Guide, CRISPR-Cas Systems, Cells, Cultured, Humans, CRISPR-Cas Systems, CRISPR-Associated Proteins
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