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Effects of fluoride on bacterial growth and its gene/protein expression
Abstract
To determine the effects of fluoride on bacterial growth, as well as upon its gene/protein expression, we grew Escherichia coli expressing GFPuv (E. coli-GFPuv) in Luria Bertani medium at different concentrations of NaF, 0, 0.1 mM, 1 mM, 10 mM and 100 mM. Results showed that E. coli-pGFPuv growth and expression of mRNA and protein of GFPuv were increased at 0.1 and 1 mM, but were inhibited at 10 and 100 mM, which demonstrated that fluoride has a classic rise/fall response of inducing E. coli-GFPuv growth and gene and protein expression of GFPuv at 1 mM. Our observation suggests that the effect of fluoride on bacterial growth may be from regulation of mRNA expression.
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“Six studies50,51,55,58,67,71 used real-time–quantitative polymerase chain reaction using 16S rRNA-specific primers to quantify bacterial species.”
From Effect of Fluoride on Gut Microbiota: A Systematic Review (2025) · cites this paper as [71]
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Research works printed but not linked: 24
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Cited by
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Calero, P., Gurdo, N., & Nikel, P. I. (2022) · Environmental Microbiology · doi:10.1111/1462-2920.16110 · Cited by 56
Tamura, M., Cueno, M. E., Abe, K., Kamio, N., Ochiai, K., & Imai, K. (2018) · Cell Stress and Chaperones · doi:10.1007/s12192-018-0922-1 · Cited by 37
Effect of Fluoride on Gut Microbiota: A Systematic Review
Yasin, M., Zohoori, F. V., Kumah, E. A., Subramanian, M., Dean, P., & Orr, C. H. (2025) · Nutrition Reviews · doi:10.1093/nutrit/nuae202 · Cited by 8
Metadata
- Authors
- Ma, H., Wu, X., Yang, M., Wang, J., Wang, J., & Wang, J.
- Venue
- Chemosphere
- Publisher
- Elsevier BV
- Published
- 2014-04
- Volume
- 100
- Pages
- 190-193
- ISSN
- 0045-6535
- Cited by
- 16
- References
- 0
- Language
- en
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