Cited by 14Plant Stress Responses and TolerancePlant Growth Enhancement TechniquesPlant-Microbe Interactions and Immunity

Deciphering the role of nitric oxide in mitigation of systemic fungicide induced growth inhibition and oxidative damage in wheat

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Touzout, Nabil, Mihoub, Adil, Ahmad, Iftikhar, Jamal, Aftab, Danish, Subhan·2024·Chemosphere

Abstract

Consento (CON) poses a significant environmental hazard as a systemic fungicide, adversely affecting the health of non-target organisms. Nitric oxide (NO), a signaling molecule, is known to play a crucial role in plant physiology and abiotic stress tolerance. However, whether NO plays any role to enhance fungicide CON tolerance in wheat seedlings is yet unclear. Therefore, we conducted a hydroponic experiment i) to investigate the morpho-physio-biochemical changes of wheat seedlings to fungicide CON stress, and ii) to examine the effects of NO and fungicide CON treatments on oxidative damage, antioxidant system, secondary metabolism and detoxification of systemic fungicide in wheat seedlings. The results showed that CON fungicide at the highest (4X) concentration significantly decreased wheat seedlings fresh weight (46.89%), shoot length (40.26%), root length (56.11%) and total chlorophyll contents (67.44%) in a dose response relationship. Moreover, CON significantly increased hydrogen peroxide, malondialdehyde, catalase, ascorbate peroxidase, glutathione-S-transferase, and peroxidase activities while decreased reduced glutathione (GSH) content. This ultimately impaired the redox homeostasis of cells, leading to oxidative damage in cell membrane. Under fungicide treatment, the addition of NO reduced the fungicide phytotoxicity, with an increase of over 60% in seedling growth. The NO application mitigated CON phytotoxicity as reflected by significantly increased chlorophyll pigments (69.88%) and decreased oxidative damage in wheat leaves. Indeed, the NO alleviatory effect was able to increase the tolerance of seedlings to fungicide, which resulted increments in antioxidant and detoxification enzymes activity, with the enhanced GSH level (78.54%). Interestingly, NO alleviated CON phytotoxicity through the phenylpropanoid pathway by enhancing the activity of secondary metabolism enzymes such as phenylalanine ammonia-lyase (47.28%), polyphenol oxidase (9%), and associated metabolites such as phenolic acids (77.62%), flavonoids (34.33%) in wheat leaves. Our study has provided evidence that NO plays a key role in the metabolism and detoxification of systemic fungicide in wheat through enhanced activity of antioxidants, detoxifications and secondary metabolic enzymes.

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Crossref · 10.1016/j.chemosphere.2024.143046

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Authors
Touzout, N., Mihoub, A., Ahmad, I., Jamal, A., & Danish, S.
Affiliations
  • University Yahia Fares of Medea (DZ)
  • CRSTRA (DZ)
  • COMSATS University Islamabad (PK)
  • The University of Agriculture, Peshawar (PK)
  • Bahauddin Zakariya University (PK)
Corresponding author
Ahmad, Iftikhar
Venue
Chemosphere
Publisher
Elsevier BV
Published
2024-09
Volume
364
Pages
143046
ISSN
0045-6535 (ISSN-L)
OpenAlex
W4401424808
Cited by
14
References
97
Citations by year
2026: 4 · 2025: 9 · 2024: 1
FWCI
3.43
Type
journal article
Language
en
Open access
Locations
  • Chemosphere (publishedVersion)
  • SSRN Electronic Journal (acceptedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Growth Enhancement Techniques
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
Keywords
nitric oxide, systemic fungicides, fungicide phytotoxicity, wheat seedlings, oxidative stress, antioxidant enzymes, glutathione, phenylpropanoid pathway, secondary metabolism, phenolic compounds, flavonoids, detoxification, redox homeostasis, chlorophyll content
MeSH terms
Antioxidants, Catalase, Chlorophyll, Fungicides, Industrial, Glutathione, Hydrogen Peroxide, Malondialdehyde, Nitric Oxide, Triticum, Oxidative Stress, Seedlings

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