Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents
Abstract
Several details of the assay of total phenolic substances have been investigated and an improved procedure developed. The improvements include the use of Folin-Ciocalteu reagent rather than the Folin-Denis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period. The values obtained are less subject to variation and interference from several nonphenols, yet are directly comparable to the "tannin" values obtained by the previously standard method.
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Total polyphenols were measured using the Folin-Ciocalteau method with catechin as a standard.
“Total polyphenols were measured in the tea, herb teas and cocoa beverages using the Folin-Ciocalteau method (Singleton & Rossi, 1965) with catechin as a standard.”
From Inhibition of non-haem iron absorption in man by polyphenolic-containing beverages (1999) · cites this paper as Singleton & Rossi, 1965
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“These values are close to those reported by Tovar et al. (2011) ( = 0.”
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de Oliveira, G. V., Morgado, M., Pierucci, A. P., & Alvares, T. S. (2016) · Journal of Functional Foods · doi:10.1016/j.jff.2016.08.017 · Cited by 45 · see citing works
Drinić, Z., Vladic, J., Koren, A., Zeremski, T., Stojanov, N., Tomić, M., & Vidović, S. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.113908 · Cited by 46 · see citing works
- Authors
- Singleton, V. L., & Rossi, J. A.
- Affiliations
- University of California, Davis (US)
- Venue
- American Journal of Enology and Viticulture
- Published
- 1965
- Volume
- 16
- Issue
- 3
- Pages
- 144-158
- ISSN
- 0002-9254 (ISSN-L)
- OpenAlex
- W2114004260
- Cited by
- 24,852
- References
- 0
- Citations by year
- 2026: 1532 · 2025: 1661 · 2024: 1621 · 2023: 1674 · 2022: 1709 · 2021: 1740 · 2020: 1671 · 2019: 1417 · 2018: 1234 · 2017: 1211 · 2016: 1123 · 2015: 1136 · 2014: 1195 · 2013: 1016 · 2012: 912
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Enology and Viticulture (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- total phenolic content, phosphomolybdic acid, phosphotungstic acid, Folin-Ciocalteu reagent, colorimetric assay, assay reproducibility
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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