Cited by 24,852Phytochemicals and Antioxidant ActivitiesFermentation and Sensory AnalysisFood and Agricultural Sciences

Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents

Singleton, V. L., Rossi, Joseph A.·1965·American Journal of Enology and Viticulture

Abstract

Several details of the assay of total phenolic substances have been investigated and an improved procedure developed. The improvements include the use of Folin-Ciocalteu reagent rather than the Folin-Denis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period. The values obtained are less subject to variation and interference from several nonphenols, yet are directly comparable to the "tannin" values obtained by the previously standard method.

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Crossref · 10.5344/ajev.1965.16.3.144

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  • Total polyphenols were measured using the Folin-Ciocalteau method with catechin as a standard.

    “Total polyphenols were measured in the tea, herb teas and cocoa beverages using the Folin-Ciocalteau method (Singleton & Rossi, 1965) with catechin as a standard.”

    From Inhibition of non-haem iron absorption in man by polyphenolic-containing beverages (1999) · cites this paper as Singleton & Rossi, 1965

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Authors
Singleton, V. L., & Rossi, J. A.
Affiliations
University of California, Davis (US)
Venue
American Journal of Enology and Viticulture
Published
1965
Volume
16
Issue
3
Pages
144-158
ISSN
0002-9254 (ISSN-L)
OpenAlex
W2114004260
Cited by
24,852
References
0
Citations by year
2026: 1532 · 2025: 1661 · 2024: 1621 · 2023: 1674 · 2022: 1709 · 2021: 1740 · 2020: 1671 · 2019: 1417 · 2018: 1234 · 2017: 1211 · 2016: 1123 · 2015: 1136 · 2014: 1195 · 2013: 1016 · 2012: 912
FWCI
1.40
Type
journal article
Language
en
Open access
closed
Locations
American Journal of Enology and Viticulture (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
total phenolic content, phosphomolybdic acid, phosphotungstic acid, Folin-Ciocalteu reagent, colorimetric assay, assay reproducibility

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