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Translocation of a Vibrio cholerae Type VI Secretion Effector Requires Bacterial Endocytosis by Host Cells
Open accessAbstract
SUMMARY The type VI secretion system (T6SS) is a virulence mechanism common to several Gram-negative pathogens. In Vibrio cholerae, VgrG-1 is required for T6SS-dependent secretion. VgrG-1 is also secreted by T6SS and displays a C-terminal actin cross-linking domain (ACD). Using a heterologous reporter enzyme in place of the ACD, we show that the effector and secretion functions of VgrG-1 are genetically dissociable with the ACD being dispensable for secretion, but required for T6SS-dependent phenotypes. Furthermore, internalization of bacteria is required for ACD translocation into phagocytic target cells. Inhibiting bacterial uptake abolishes actin cross-linking while improving intracellular survival enhances it. Otherwise resistant nonphagocytic cells become susceptible to T6SS-mediated actin cross-linking when engineered to take up bacteria. Our results support a model for translocation of VgrG C-terminal effector domains into target cell cytosol by a process that requires trafficking of bacterial cells into an endocytic compartment where translocation is triggered by an unknown signal.
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Cited by
A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to Bacteria
Hood, R. D., Singh, P., Hsu, F., Güvener, T., Carl, M. A., Trinidad, R. R. S., Silverman, J. M., Ohlson, B. B., Hicks, K. G., Plemel, R. L., et al. (2010) · Cell Host & Microbe · doi:10.1016/j.chom.2009.12.007 · Cited by 932
A View to a Kill: The Bacterial Type VI Secretion System
Ho, B. T., Dong, T. G., & Mekalanos, J. J. (2014) · Cell Host & Microbe · doi:10.1016/j.chom.2013.11.008 · Cited by 639
Self-citationJiang, F., Waterfield, N. R., Yang, J., Yang, G., & Jin, Q. (2014) · Cell Host & Microbe · doi:10.1016/j.chom.2014.04.010 · Cited by 270
Cao, Z., Casabona, M. G., Kneuper, H., Chalmers, J. D., & Palmer, T. (2016) · Nature Microbiology · doi:10.1038/nmicrobiol.2016.183 · Cited by 264
Architecture and assembly of the Type VI secretion system
Zoued, A., Brunet, Y. R., Durand, E., Aschtgen, M.-S., Logger, L., Douzi, B., Journet, L., Cambillau, C., & Cascales, E. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2014.03.018 · Cited by 259
Metadata
- Authors
- Ma, A. T., McAuley, S., Pukatzki, S., & Mekalanos, J. J.
- Venue
- Cell Host & Microbe
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 5
- Issue
- 3
- Pages
- 234-243
- ISSN
- 1931-3128 (ISSN-L)
- OpenAlex
- W2007960706
- Cited by
- 284
- References
- 0
- FWCI
- 16.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ma, A. T., McAuley, S., Pukatzki, S., & Mekalanos, J. J. (2009). Translocation of a Vibrio cholerae Type VI Secretion Effector Requires Bacterial Endocytosis by Host Cells. Cell Host & Microbe. https://doi.org/10.1016/j.chom.2009.02.005
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