Fast high performance liquid chromatography and ultraviolet–visible quantification of principal phenolic antioxidants in fresh rosemary
Abstract
An improved HPLC method is reported for the determination of rosemary's principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV-vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
For R. officinalis, the phenolic diterpenes carnosic acid and carnosol are considered important chemotaxonomic markers.
“and, for R. officinalis, the phenolic diterpenes carnosic acid and carnosol are also considered important (Mena et al., 2016;Troncoso et al., 2005;Yeddes et al., 2019;Yilmaz et al., 2018;Zhang et al., 2012).”
From Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease (2022) · cites this paper as Troncoso et al., 2005
Cites
Petersen, M. (2002) · Phytochemistry · doi:10.1016/s0031-9422(02)00513-7 · Cited by 1,265 · see citing works
Antioxidants/antimutagens in food
Namiki, M. (1990) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408399009527528 · Cited by 903 · see citing works
Madsen, H. L., & Bertelsen, G. (1995) · Trends in Food Science & Technology · doi:10.1016/s0924-2244(00)89112-8 · Cited by 615 · see citing works
Frankel, E. N., Huang, S., Aeschbach, R., & Prior, E. M. (1996) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf950374p · Cited by 550 · see citing works
Antioxidant and pro-oxidant properties of active rosemary constituents: carnosol and carnosic acid
Aruoma, O. I., Halliwell, B. B., Aeschbach, R., & Löligers, J. (1992) · Xenobiotica · doi:10.3109/00498259209046624 · Cited by 514 · see citing works
Antioxidant Constituents in Sage (Salvia officinalis)
Cuvelier, M. E., Berset, C., & Richard, H. M. J. (1994) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00039a012 · Cited by 411 · see citing works
del Baño, M. J., Lorente, J., Castillo, J., Benavente-García, O., del Rı́o, J. A., Ortuño, A. M., Quirin, K., & Gerard, D. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0300745 · Cited by 368 · see citing works
Pizzale, L., Bortolomeazzi, R., Vichi, S., Überegger, E., & Conte, L. S. (2002) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1240 · Cited by 281 · see citing works
Miura, K., Kikuzaki, H., & Nakatani, N. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf011314o · Cited by 276 · see citing works
Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves
Herodež, Š. S., Hadolin, M., Škerget, M., et al. (2002) · Food Chemistry · doi:10.1016/s0308-8146(02)00382-5 · Cited by 231 · see citing works
Evaluation of antioxidant activity of some plant extracts and their application in biscuits
Reddy, V., Urooj, A., & Kumar, A. K. (2004) · Food Chemistry · doi:10.1016/j.foodchem.2004.05.038 · Cited by 222 · see citing works
Chen, Q., Shi, H., & Ho, C. (1992) · Journal of the American Oil Chemists Society · doi:10.1007/bf02541065 · Cited by 190 · see citing works
The use of natural antioxidants in ready-to-serve food
Karpińska, M., Borowski, J., & Danowska‐Oziewicz, M. (2001) · Food Chemistry · doi:10.1016/s0308-8146(00)00171-0 · Cited by 163 · see citing works
Munné‐Bosch, S., Nogués, S., & Alegre, L. (1999) · New Phytologist · doi:10.1046/j.1469-8137.1999.00490.x · Cited by 154 · see citing works
Wellwood, C. R. L., & Cole, R. A. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf035335p · Cited by 131 · see citing works
Quantitative determination of phenolic diterpenes in rosemary extracts
Thorsen, M. A., & Hildebrandt, K. S. (2003) · Journal of Chromatography A · doi:10.1016/s0021-9673(03)00487-4 · Cited by 127 · see citing works
Nissen, L. R., Månsson, L., Bertelsen, G., et al. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0003552 · Cited by 98 · see citing works
Flavonoids and Phenolic Acids of Sage: Influence of Some Agricultural Factors
Areias, F. M., Valentão, P., Andrade, P. B., Ferreres, F., & Seabra, R. M. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000440+ · Cited by 88 · see citing works
Natural antioxidants in functional foods: from food safety to health benefits
Alfonso Valenzuela, B., Julio Sanhueza, C., & Nieto, S. (2003) · Grasas y Aceites · doi:10.3989/gya.2003.v54.i3.245 · Cited by 75 · see citing works
Food antioxidants and phytochemicals: present and future perspectives
Frankel, E. N. (1999) · Fette Seifen Anstrichmittel · doi:10.1002/(sici)1521-4133(199912)101:12 · Cited by 61 · see citing works
Ragno, G., Ioele, G., & Risoli, A. (2004) · Analytica Chimica Acta · doi:10.1016/j.aca.2004.02.034 · Cited by 53 · see citing works
Yuan, J., Chen, H., & Chen, F. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980088o · Cited by 23 · see citing works
IMPROVEMENT OF MULTIVARIATE UV ANALYSIS BY SELECTING WAVELENGTHS AND INSTRUMENTAL PARAMETERS
Vetuschi, C., & Ragno, G. (2002) · Analytical Letters · doi:10.1081/al-120002687 · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Chromatographic Separation Techniques and Data Handling Methods for Herbal Fingerprints: A Review
Tistaert, C., Dejaegher, B., & Heyden, Y. V. (2011) · Analytica Chimica Acta · doi:10.1016/j.aca.2011.02.023 · Cited by 341 · see citing works
Yeddes, W., Chalghoum, A., Aidi-Wannes, W., Ksouri, R., & Saidani Tounsi, M. (2019) · Journal of Essential Oil Research · doi:10.1080/10412905.2019.1577305 · Cited by 49 · see citing works
Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease
Fernandes, F., Barroso, M. F., De Simone, A., Emriková, E., Dias-Teixeira, M., Pereira, J. P., Chlebek, J., Fernandes, V. C., Rodrigues, F., Andrisano, V., et al. (2022) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2022.115107 · Cited by 45 · see citing works
- Authors
- Troncoso, N., Sierra, H., Carvajal, L., Delpiano, P., & Günther, G.
- Affiliations
- Fundación Chile (CL)
- University of Chile (CL)
- Corresponding author
- Günther, G.
- ORCID
- Günther, G.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 2005-12
- Volume
- 1100
- Issue
- 1
- Pages
- 20-25
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W2008300272
- Cited by
- 74
- References
- 23
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 1 · 2013: 3 · 2012: 3
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- PubMed (publishedVersion)
- Universidad de Chile (submittedVersion)
- Funders
- Corporación de Fomento de la Producción
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Keywords
- Rosmarinus officinalis, rosmarinic acid, carnosic acid, phenolic antioxidants, HPLC method optimization, solid-phase extraction, methanol extraction
- MeSH terms
- Antioxidants, Chromatography, High Pressure Liquid, Phenols, Plants, Edible, Spectrophotometry, Ultraviolet
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
