Biobanking of CSF: International standardization to optimize biomarker development
Abstract
Cerebrospinal fluid (CSF) reflects pathophysiological aspects of neurological diseases, where neuroprotective strategies and biomarkers are urgently needed. Therefore, biobanking is very relevant for biomarker discovery and evaluation of neurological diseases. Important and unique features of CSF biobanking are intensive collaboration in international networks and the tight application of standardized protocols. The current adoption of standardized protocols for CSF and blood collection as presented in this review enables biomarker studies in large cohorts of patients and controls. Another topic of this review is the selection of control groups, which influences the outcome of biomarker investigations. Control groups in CSF biobanks mainly consist of different disease controls. This is in part due to the fact that lumbar punctures are mostly performed for clinical indications and rarely for research purposes only, as it is a relatively invasive procedure. Moreover, there is a lack of homogenous criteria and definition of control groups. We therefore propose uniform consensus definitions for such control groups in biomarker research, i.e. Healthy controls (HC), Spinal anesthesia subjects (SAS), Symptomatic controls (SC), Inflammatory Neurological Disease Controls (CINDC), Peripheral Inflammatory Neurological Disease Controls (PINDC) and Non-inflammatory Neurological Disease Controls (NINDC). Another important aspect of CSF biobanking is quality control. Systematic studies to a…
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CSF sample collection and processing followed standard procedures.
“The CSF sample collection and processing followed standard procedures25 and have been described previously15.”
From Plasma p-tau231 and p-tau217 as state markers of amyloid-β pathology in preclinical Alzheimer’s disease (2022) · cites this paper as [25]
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Self-citationCystatin F is a biomarker of prion pathogenesis in mice
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- Authors
- Teunissen, C. E., Tumani, H., Engelborghs, S., & Mollenhauer, B.
- Affiliations
- Amsterdam Neuroscience (NL)
- University Hospital Ulm (DE)
- University of Antwerp (BE)
- Universitätsmedizin Göttingen (DE)
- Corresponding author
- Teunissen, Charlotte E.
- Venue
- Clinical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2014-03
- Volume
- 47
- Issue
- 4-5
- Pages
- 288-292
- ISSN
- 0009-9120 (ISSN-L)
- OpenAlex
- W2047101604
- Cited by
- 137
- References
- 1
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 19 · 2023: 12 · 2022: 13 · 2021: 16 · 2020: 11 · 2019: 13 · 2018: 10 · 2017: 4 · 2016: 4 · 2015: 13 · 2014: 2
- FWCI
- 3.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- GRO (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- EU Joint Programme – Neurodegenerative Disease Research
- Bundesministerium für Bildung und Forschung
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- biomarker development, neurological disorders, lumbar puncture, pre-analytical variability, cerebrospinal fluid protein, neurodegenerative diseases, international standardization
- MeSH terms
- Cooperative Behavior, Humans, Quality Control, Research Design, Specimen Handling, Biomarkers, Guidelines as Topic, Biological Specimen Banks, Controlled Clinical Trials as Topic, Neurodegenerative Diseases, Consensus, Control Groups
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