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Cited by 180Pancreatic function and diabetesIon channel regulation and functionIon Transport and Channel Regulation

Kv2.1 Ablation Alters Glucose-Induced Islet Electrical Activity, Enhancing Insulin Secretion

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Jacobson, David A., Kuznetsov, Andrey, Lopez, James P., Kash, Shera, Ämmälä, Carina E., Philipson, Louis H.·2007·Cell Metabolism
DOI 10.1016/j.cmet.2007.07.010PMC PMC2699758
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Abstract

Voltage-gated potassium currents (Kv), primarily due to Kv2.1 channels, are activated by glucose-stimulated pancreatic beta cell depolarization, but the exact role (or roles) of this channel in regulating insulin secretion remains uncertain. Here we report that, compared with controls, Kv2.1 null mice have reduced fasting blood glucose levels and elevated serum insulin levels. Glucose tolerance is improved and insulin secretion is enhanced compared to control animals, with similar results in isolated islets in vitro. Isolated Kv2.1(-/-) beta cells have residual Kv currents, which are decreased by 83% at +50 mV compared with control cells. The glucose-induced action potential (AP) duration is increased while the firing frequency is diminished, similar to the effect of specific toxins on control cells but substantially different from the effect of the less specific blocker tetraethylammonium. These results reveal the specific role of Kv2.1 in modulating glucose-stimulated APs of beta cells, exposing additional important currents involved in regulating physiological insulin secretion.

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  • Experiments on Kcnb1-deficient mice make it clear that Kv2.1 channels account for most of the delayed outward current in mouse β-cells.

    “However, experiments on Kcnb1-deficient mice make it clear that these channels account for most of the delayed outward current in mouse β-cells (310).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [310]

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Authors
Jacobson, D. A., Kuznetsov, A., Lopez, J. P., Kash, S., Ämmälä, C. E., & Philipson, L. H.
Affiliations
  • University of Chicago (US)
  • Deltagen (United States) (US)
  • GlaxoSmithKline (United States) (US)
Corresponding author
  • Jacobson, David A.
  • Philipson, Louis H.
ORCID
Venue
Cell Metabolism
Publisher
Elsevier BV
Published
2007-09
Volume
6
Issue
3
Pages
229-235
ISSN
1550-4131 (ISSN-L)
DOI
10.1016/j.cmet.2007.07.010
PMC
PMC2699758
OpenAlex
W2071651200
Cited by
180
References
0
Citations by year
2026: 3 · 2025: 8 · 2024: 13 · 2023: 2 · 2022: 8 · 2021: 17 · 2020: 7 · 2019: 14 · 2018: 9 · 2017: 18 · 2016: 5 · 2015: 10 · 2014: 15 · 2013: 9 · 2012: 10
FWCI
7.28
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (R01DK048494, DK 20595, DK-48494, P60DK020595, P30DK020595)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
Keywords
Kv2.1 potassium channel, voltage-gated potassium currents, pancreatic beta cells, insulin secretion, glucose tolerance, tetraethylammonium
MeSH terms
Animals, Calcium, Female, Glucose, Homeostasis, Humans, Insulin, Islets of Langerhans, Membrane Potentials, Mice, Inbred C57BL, Mice, Knockout, Patch-Clamp Techniques, Mice, Shab Potassium Channels

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