The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Metabolic Pitfalls of CNS Cre-Based Technology
Open accessAbstract
Nestin-Cre mice have a significant metabolic phenotype that is hard to discern from current literature. Indeed, the Cre-lox system has numerous problems that can affect physiological parameters, and these are missed when the correct control strains are not used. Despite the increasing use of the Cre-lox system, these issues were not visible to the scientific community previously and may have affected published work. This makes it important to highlight the issues and raise awareness of the pitfalls of the Cre-lox system. Therefore, this perspective will discuss the impact of CNS and peripheral "off-target" Cre recombination on metabolic systems and describe the development of new approaches to obviate the difficulties.
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“However, these studies were confounded by the fact that ap2-Cre transgenic mice also express Cre recombinase in nonadipose tissues, including in the CNS (47).”
From Fibroblast Growth Factor 21: A Versatile Regulator of Metabolic Homeostasis (2018) · cites this paper as [47]
Citations
Cites
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Nestin-expressing progenitor cells: function, identity and therapeutic implications
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A novel m6A reader Prrc2a controls oligodendroglial specification and myelination
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FGF21 Regulates Metabolism Through Adipose-Dependent and -Independent Mechanisms
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Growth hormone regulates neuroendocrine responses to weight loss via AgRP neurons
Furigo, I. C., Teixeira, P. D. S., de Souza, G. O., Couto, G. C. L., Romero, G. G., Perelló, M., Frazão, R., Elias, L. L. K., Metzger, M., List, E. O., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-08607-1 · Cited by 230
Metadata
- Authors
- Harno, E., Cottrell, E. C., & White, A.
- Affiliations
- University of Manchester (GB)
- Corresponding author
- White, Anne
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 18
- Issue
- 1
- Pages
- 21-28
- ISSN
- 1550-4131 (ISSN-L)
- OpenAlex
- W2093032500
- Cited by
- 200
- References
- 64
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 5 · 2023: 14 · 2022: 17 · 2021: 13 · 2020: 21 · 2019: 25 · 2018: 18 · 2017: 20 · 2016: 16 · 2015: 18 · 2014: 12 · 2013: 5
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Funders
- AstraZeneca
- National Institute for Health and Care Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- Cre-loxP system, central nervous system, metabolic phenotypes, control strains, conditional gene targeting
- MeSH terms
- Animals, Central Nervous System, DNA Nucleotidyltransferases, Protein-Lysine 6-Oxidase, Mice, Transgenic, Recombination, Genetic, Extracellular Matrix Proteins, Mice, Knockout, Gene Targeting, Integrases, Models, Animal, Mice, Nestin
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