Metabolic Pitfalls of CNS Cre-Based Technology

A robust and high-throughput Cre reporting and characterization system for the whole mouse brain

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Generalized lacZ expression with the ROSA26 Cre reporter strain

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Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety

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Rapid, reversible activation of AgRP neurons drives feeding behavior in mice

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Genetic transformation of mouse embryos by microinjection of purified DNA.

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Behavioral phenotypes of inbred mouse strains: implications and recommendations for molecular studies

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Remote Control of Neuronal Activity in Transgenic Mice Expressing Evolved G Protein-Coupled Receptors

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Hypoxia-Inducible Factor 1α Induces Fibrosis and Insulin Resistance in White Adipose Tissue

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Talking about a Revolution

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Bacteriophage P1 site-specific recombination

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Immune responses to gene therapy vectors: influence on vector function and effector mechanisms

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Neuronal PTP1B regulates body weight, adiposity and leptin action

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Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells

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Fate mapping Nkx2.1‐lineage cells in the mouse telencephalon

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Introduction of a rabbit β-globin gene into the mouse germ line

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Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells

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Disruption of neural signal transducer and activator of transcription 3 causes obesity, diabetes, infertility, and thermal dysregulation

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Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids

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Epithelial-Specific Cre/lox Recombination in the Developing Kidney and Genitourinary Tract

Shao, X., Somlo, S., & Igarashi, P. (2002) · Journal of the American Society of Nephrology · doi:10.1097/01.asn.0000016444.90348.50 · Cited by 332

Lessons on Conditional Gene Targeting in Mouse Adipose Tissue

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Pomc-expressing progenitors give rise to antagonistic neuronal populations in hypothalamic feeding circuits

Padilla, S. L., Carmody, J. S., & Zeltser, L. M. (2010) · Nature Medicine · doi:10.1038/nm.2126 · Cited by 312

Mammalian genomes contain active recombinase recognition sites

Thyagarajan, B., Guimarães, M. J., Groth, A. C., & Calos, M. P. (2000) · Gene · doi:10.1016/s0378-1119(00)00008-1 · Cited by 289

Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types

Schonhoff, S. E., Giel–Moloney, M., & Leiter, A. B. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.03.013 · Cited by 285

RIP-Cre Revisited, Evidence for Impairments of Pancreatic β-Cell Function

Lee, J., Ristow, M., Lin, X., White, M. F., Magnuson, M. A., & Hennighausen, L. G. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m512373200 · Cited by 252

Inhibition of growth hormone action improves insulin sensitivity in liver IGF-1–deficient mice

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Delivery of the Cre recombinase by a self-deleting lentiviral vector: Efficient gene targeting in vivo

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Sonic Hedgehog Signaling Confers Ventral Telencephalic Progenitors with Distinct Cortical Interneuron Fates

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Inhibition of growth hormone action improves insulin sensitivity in liver IGF-1–deficient mice

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Adeno-associated virus effectively mediates conditional gene modification in the brain

Kaspar, B. K., Vissel, B., Bengoechea, T. G., Crone, S. A., Randolph‐Moore, L., Müller, R., Brandon, E. P., Schaffer, D. V., Verma, I. M., Lee, K., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.042678699 · Cited by 196

Pancreas-Specific Cre Driver Lines and Considerations for Their Prudent Use

Magnuson, M. A., & Osipovich, A. B. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.06.011 · Cited by 182

Nestin‐Cre transgenic mouse line Nes‐Cre1 mediates highly efficient Cre/loxP mediated recombination in the nervous system, kidney, and somite‐derived tissues

Dubois, N. C., Hofmann, D., Kaloulis, K., Bishop, J. M., & Trumpp, A. (2006) · genesis · doi:10.1002/dvg.20226 · Cited by 181

Conditional gene targeting on the pure C57BL/6 genetic background

Mishina, M., & Sakimura, K. (2007) · Neuroscience Research · doi:10.1016/j.neures.2007.01.004 · Cited by 175

Conditional transgenic technologies.

Ryding, A., Sharp, M., & Mullins, J. J. (2001) · Journal of Endocrinology · doi:10.1677/joe.0.1710001 · Cited by 171

High Levels of Cre Expression in Neuronal Progenitors Cause Defects in Brain Development Leading to Microencephaly and Hydrocephaly

Forni, P. E., Scuoppo, C., Imayoshi, I., Taulli, R., Dastrù, W., Sala, V., Betz, U. A. K., Muzzi, P., Martinuzzi, D., Vercelli, A., et al. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.2815-06.2006 · Cited by 167

Neural crest expression of Cre recombinase directed by the proximal Pax3 promoter in transgenic mice

Li, J., Chen, F., & Epstein, J. A. (2000) · genesis · doi:10.1002/(sici)1526-968x(200002)26:2 · Cited by 158

Cre activity causes widespread apoptosis and lethal anemia during embryonic development

Naiche, L. A., & Papaioannou, V. E. (2007) · genesis · doi:10.1002/dvg.20353 · Cited by 148

The advent of AAV9 expands applications for brain and spinal cord gene delivery

Dayton, R. D., Wang, D. B., & Klein, R. L. (2012) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2012.681463 · Cited by 144

Nestin expression in pancreatic exocrine cell lineages

Delacour, A., Népote, V., Trumpp, A., & Herrera, P. L. (2003) · Mechanisms of Development · doi:10.1016/j.mod.2003.11.004 · Cited by 142

Tamoxifen-Induced Cre-loxP Recombination Is Prolonged in Pancreatic Islets of Adult Mice

Reinert, R. B., Kantz, J., Misfeldt, A. A., Poffenberger, G., GANNON, M. A., Briššová, M., & Powers, A. C. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033529 · Cited by 140

Defining POMC Neurons Using Transgenic Reagents: Impact of Transient Pomc Expression in Diverse Immature Neuronal Populations

Padilla, S. L., Reef, D. K., & Zeltser, L. M. (2011) · Endocrinology · doi:10.1210/en.2011-1665 · Cited by 120

Deficiency of corpus callosum varies with strain and supplier of the mice

Wahłsten, D. (1982) · Brain Research · doi:10.1016/0006-8993(82)90513-3 · Cited by 114

Growth Hormone Induces Cellular Insulin Resistance by Uncoupling Phosphatidylinositol 3-Kinase and Its Downstream Signals in 3T3-L1 Adipocytes

Takano, A., Haruta, T., Iwata, M., Usui, I., Uno, T., Kawahara, J., Ueno, E., Sasaoka, T., & Kobayashi, M. (2001) · Diabetes · doi:10.2337/diabetes.50.8.1891 · Cited by 109

A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors

Liang, H., Hippenmeyer, S., & Ghashghaei, H. T. (2012) · Biology Open · doi:10.1242/bio.20122287 · Cited by 107

Brain expression of Cre recombinase driven by pancreas‐specific promoters

Song, J., Xu, Y., Hu, X., Choi, B. J., & Tong, Q. (2010) · genesis · doi:10.1002/dvg.20672 · Cited by 107

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

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Ectopic recombination in the central and peripheral nervous system by aP2/FABP4‐Cre mice: Implications for metabolism research

Martens, K., Bottelbergs, A., & Baes, M. I. (2010) · FEBS Letters · doi:10.1016/j.febslet.2010.01.061 · Cited by 100

Genetically manipulated mice: a powerful tool with unsuspected caveats

Matthaei, K. I. (2007) · The Journal of Physiology · doi:10.1113/jphysiol.2007.134908 · Cited by 96

The 5'-flanking region of the rat synapsin I gene directs neuron-specific and developmentally regulated reporter gene expression in transgenic mice.

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Synapsin I Cre transgene expression in male mice produces germline recombination in progeny

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The optogenetic (r)evolution

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A novel adipose-specific gene deletion model demonstrates potential pitfalls of existing methods

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Nestin-Cre Mice Are Affected by Hypopituitarism, Which Is Not Due to Significant Activity of the Transgene in the Pituitary Gland

Galichet, C., Lovell-Badge, R. H., & Rizzoti, K. (2010) · PLoS ONE · doi:10.1371/journal.pone.0011443 · Cited by 76

Combined Neural Inactivation of Suppressor of Cytokine Signaling-3 and Protein-Tyrosine Phosphatase-1B Reveals Additive, Synergistic, and Factor-Specific Roles in the Regulation of Body Energy Balance

Briançon, N., McNay, D. E. G., Maratos–Flier, E., & Flier, J. S. (2010) · Diabetes · doi:10.2337/db10-0481 · Cited by 61

A chemical-genetic approach for precise spatio-temporal control of cellular signaling

Dong, S., Allen, J., Farrell, M. S., & Roth, B. L. (2010) · Molecular BioSystems · doi:10.1039/c002568m · Cited by 55

Identification of Epidermal Pdx1 Expression Discloses Different Roles of Notch1 and Notch2 in Murine KrasG12D-Induced Skin Carcinogenesis In Vivo

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Neuron-Specific Deletion of Peroxisome Proliferator-Activated Receptor Delta (PPARδ) in Mice Leads to Increased Susceptibility to Diet-Induced Obesity

Kocalis, H. E., Turney, M. K., Printz, R. L., Laryea, G. N., Muglia, L. J., Davies, S. S., Stanwood, G. D., McGuinness, O. P., & Niswender, K. D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0042981 · Cited by 35

Conditional deletion of histone deacetylase‐4 in the central nervous system has no major effect on brain architecture or neuronal viability

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A Non-Specific Effect Associated with Conditional Transgene Expression Based on Cre-loxP Strategy in Mice

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Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line

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Hypoxia‐inducible factor 1 activation from adipose protein 2‐cre mediated knockout of von hippel‐lindau gene leads to embryonic lethality

Zhang, J., Wang, Y., Gao, Z., Yun, Z., & Ye, J. (2011) · Clinical and Experimental Pharmacology and Physiology · doi:10.1111/j.1440-1681.2011.05656.x · Cited by 20

Molecular biology and biotechnology

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Rat Synapsin 1 Promoter Mediated Transgene Expression in Testicular Cell Types

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A pink mouse reports the switch from red to green fluorescence upon Cre-mediated recombination

Hartwich, H., Satheesh, S. V., & Nothwang, H. G. (2012) · BMC Research Notes · doi:10.1186/1756-0500-5-296 · Cited by 7

Addendum: Neuronal PTP1B regulates body weight, adiposity and leptin action

Bence, K. K., Delibegović, M., Xue, B., Görgün, C. Z., Hotamışlıgil, G. S., Neel, B. G., & Kahn, B. B. (2010) · Nature Medicine · doi:10.1038/nm0210-237b · Cited by 2

Nestin-expressing progenitor cells: function, identity and therapeutic implications

Bernal, A., & Arranz, L. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-018-2794-z · Cited by 415

A novel m6A reader Prrc2a controls oligodendroglial specification and myelination

Wu, R., Li, A., Sun, B., Sun, J.-G., Zhang, J., Zhang, T., Chen, Y., Xiao, Y., Gao, Y., Zhang, Q., et al. (2019) · Cell Research · doi:10.1038/s41422-018-0113-8 · Cited by 366

FGF21 Regulates Metabolism Through Adipose-Dependent and -Independent Mechanisms

BonDurant, L. D., Ameka, M., Naber, M. C., Markan, K. R., Idiga, S. O., Acevedo, M. R., Walsh, S. A., Ornitz, D. M., & Potthoff, M. J. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.03.005 · Cited by 330

The glucose-dependent insulinotropic polypeptide (GIP) regulates body weight and food intake via CNS-GIPR signaling

Zhang, Q., Delessa, C. T., Augustin, R., Bakhti, M., Colldén, G., Drucker, D. J., Feuchtinger, A., Caceres, C. G., Grandl, G., Harger, A., et al. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.01.015 · Cited by 322

Growth hormone regulates neuroendocrine responses to weight loss via AgRP neurons

Furigo, I. C., Teixeira, P. D. S., de Souza, G. O., Couto, G. C. L., Romero, G. G., Perelló, M., Frazão, R., Elias, L. L. K., Metzger, M., List, E. O., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-08607-1 · Cited by 230

Nestin-Cre Mouse Lines: A User Guide for CNS Genetics

Thomas, M., & Wei, P. (2026) · Journal of Neuroscience · doi:10.1523/jneurosci.0537-26.2026

Pitfalls of PCR-Based Strategy for Genotyping Cre-Loxp Mice

Zhang, J., Liu, W., Ye, P., & D’Ercole, A. J. (2007) · BioTechniques · doi:10.2144/000112429 · Cited by 6

Pancreas-Specific Cre Driver Lines and Considerations for Their Prudent Use

Magnuson, M. A., & Osipovich, A. B. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.06.011 · Cited by 182

The Cre/Lox System to Assess the Development of the Mouse Brain.

Kratochwil, C., & Rijli, F. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9732-9_28 · Cited by 2

Tetracycline response element driven Cre causes ectopic recombinase activity independent of transactivator element

Lewis, K. T., Oles, L. R., & MacDougald, O. A. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101501 · Cited by 6