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Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes
Open accessAbstract
The interaction between the immune system and endocrine cells in the pancreas is crucial for the initiation and progression of type 1 diabetes (T1D). Imaging mass cytometry (IMC) enables multiplexed assessment of the abundance and localization of more than 30 proteins on the same tissue section at 1-μm resolution. Herein, we have developed a panel of 33 antibodies that allows for the quantification of key cell types including pancreatic exocrine cells, islet cells, immune cells, and stromal components. We employed this panel to analyze 12 pancreata obtained from donors with clinically diagnosed T1D and 6 pancreata from non-diabetic controls. In the pancreata from donors with T1D, we simultaneously visualized significant alterations in islet architecture, endocrine cell composition, and immune cell presentation. Indeed, we demonstrate the utility of IMC to investigate complex events on the cellular level that will provide new insights on the pathophysiology of T1D.
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Metadata
- Authors
- Wang, Y. J., Traum, D., Schug, J., Gao, L., Liu, C., Atkinson, M. A., Powers, A. C., Feldman, M. D., Naji, A., Chang, K.-M., & Kaestner, K. H.
- Affiliations
- University of Pennsylvania (US)
- Philadelphia VA Medical Center (US)
- University of Florida Health (US)
- VA Tennessee Valley Healthcare System (US)
- Corresponding author
- Kaestner, Klaus H.
- ORCID
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2019-03
- Volume
- 29
- Issue
- 3
- Pages
- 769-783.e4
- ISSN
- 1550-4131 (ISSN-L)
- PMC
- PMC6436557
- OpenAlex
- W2913147009
- Cited by
- 228
- References
- 51
- Citations by year
- 2026: 23 · 2025: 29 · 2024: 28 · 2023: 22 · 2022: 32 · 2021: 47 · 2020: 26 · 2019: 20
- FWCI
- 18.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIDDK (UC4 DK112217, UC4 DK112232, UC4 DK108132, UC4 DK104119)
- University of Pennsylvania Diabetes Research Center (P30 DK19525)
- Vanderbilt Diabetes Research and Training Center (P30 DK20593)
- Department of Veterans Affairs
- JDRF
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- type 1 diabetes, imaging mass cytometry, pancreatic islets, endocrine pancreas, immune cells, islet architecture, pancreatic stroma, exocrine pancreas, endocrine-immune interaction
- MeSH terms
- Diabetes Mellitus, Type 1, Humans, Immune System, Islets of Langerhans, Image Cytometry, Endocrine Cells
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