Evaluation of biological response induced by molybdenum oxide nanocolloids on in vitro cultured NIH/3T3 fibroblast cells by micro-Raman spectroscopy
Abstract
Tailored colloids of uniformly sized and engineered molybdenum oxide nanoparticles were produced, for the first time, by pulsed laser ablation in water. This green technique ensures the formation of contaminant-free nanostructures and the absence of by-products, very useful issues in biological applications. A selective tuning of MoO chemical bonding configurations and a suitable control of nanoparticles size distributions were achieved during the ablation processes by varying the water temperature and by applying an external electric field. The metal redox properties are fundamental factors governing both cell uptake and interaction mode with Mo oxide nanoparticles. Micro-Raman spectroscopy was used to investigate the existence of cellular changes induced by Mo oxide colloids on the fibroblast cell line NIH/3T3 in relation to the molecular vibrations due to proteins, lipids and nucleic acids. The label-free micro-Raman spectroscopy provides an easy and noninvasive method to monitor the harmful effect of toxic agents on cells through ROS production or redox-dependent mechanisms. In view of potential biological applications, molybdenum oxide nanoparticles cytotoxicity towards NIH/3T3 cells was also investigated. A statistical analysis shows that, in the 10-100 μg/mL Mo concentration range, all the colloids are cytotoxic, progressively reducing the cell viability down to 75% upon increasing the concentration. The effect is less pronounced for the oxygen deficient MoO 3 samples where cell viability does not fall below 85%. These results open the way to identify potential bioactive products affecting cellular redox status, by using only the Raman spectral data, even before performing lengthy and expensive specific clinical analyses.
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Both Mo oxide nanocolloids induce metal redox and antioxidant properties that strongly depend on Mo-O surface chemical bonding configurations and NP morphology.
“Both nanocolloids induce metal redox and antioxidant properties which strongly depend on the Mo–O surface chemical bonding configurations and NP morphology.”
From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [141]
Mo oxide nanocolloids were synthesized by Spadaro and Fazio et al. using a focused picosecond-pulsed laser beam (λ = 532 nm, repetition rate = 100 kHz, pulse width = 6-8 ps) ablating a molybdenum target in water.
“The EF role on Mo-based nanostructures is also shown by Spadaro and Fazio et al. [140,141] that deal with the synthesis of Mo oxide nanocolloids using a focused picosecond-pulsed laser beam (λ = 532 nm, repetition rate = 100 kHz, pulse width = 6–8 ps).”
From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [141]
Selective tuning of Mo-O chemical bonding configurations and control of NP size distributions were achieved by varying water temperature and applying the external electric field.
“A selective tuning of Mo–O chemical bonding configurations and a suitable control of NP size distributions were achieved by varying the water temperature and by applying the external EF.”
From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [141]
Applying an external electric field (estimated 20 V/cm, Pt electrode distance 5 cm) during ablation was exploited to check for modifications of NP surface morphology and surface chemical bonding configurations.
“A molybdenum target was ablated in water and the effect of an external EF (the estimated electric field is 20 V/cm, the Pt electrode distance fixed at 5 cm) applied during the ablation process, has been exploited in order to check for modifications of the NP surface morphology and surface chemical bonding configurations.”
From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [141]
Two types of Mo oxide nanocolloids were prepared: one spherical with size less than 50 nm and dominant MoO2 surface chemical configuration, and another oblong where the oxygen-deficient MoO3 phase increases at the expense of MoO2.
“Two types of nanocolloids were prepared: one with a spherical shape, size less than 50 nm and a dominant MoO2 surface chemical configuration, and another with an oblong structure, where the oxygen deficient MoO3 phase increases at the expense of the MoO2 one.”
From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [141]
Cites
Molybdenum cofactor and human disease
Schwarz, G. (2016) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2016.03.016 · Cited by 115 · see citing works
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Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications
Fazio, E., Gökce, B., De Giacomo, A., Meneghetti, M., Compagnini, G., Tommasini, M., Waag, F., Lucotti, A., Zanchi, C. G., Ossi, P. M., et al. (2020) · Nanomaterials · doi:10.3390/nano10112317 · Cited by 346 · see citing works
“[140,141] that deal with the synthesis of Mo oxide nanocolloids using a focused picosecond-pulsed laser beam (λ = 532 nm, repetition rate = 100 kHz, pulse width = 6–8 ps).”
Self-citationTalik Sisin, N. N., Abdul Razak, K., Zainal Abidin, S., Che Mat, N. F., Abdullah, R., Ab Rashid, R., Khairil Anuar, M. A., & Rahman, W. N. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s269214 · Cited by 36 · see citing works
- Authors
- Fazio, E., Speciale, A., Spadaro, S., Bonsignore, M., Cimino, F., Cristani, M., Trombetta, D., Saija, A., & Neri, F.
- Affiliations
- University of Messina (IT)
- Corresponding author
- Fazio, Enza
- Speciale, Antonio
- ORCID
- Venue
- Colloids and Surfaces B: Biointerfaces
- Publisher
- Elsevier BV
- Published
- 2018-10
- Volume
- 170
- Pages
- 233-241
- ISSN
- 0927-7765 (ISSN-L)
- OpenAlex
- W2809434316
- Cited by
- 31
- References
- 1
- Citations by year
- 2025: 5 · 2024: 5 · 2022: 2 · 2021: 3 · 2020: 7 · 2019: 8 · 2018: 1
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Colloids and Surfaces B Biointerfaces (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Laser-Ablation Synthesis of Nanoparticles
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- molybdenum oxide nanoparticles, molybdenum trioxide, green synthesis, nanoparticle size control, electric field control, cytotoxicity, NIH3T3 cells, cell viability, redox state, reactive oxygen species, label-free cell monitoring, micro-Raman spectroscopy
- MeSH terms
- Animals, Cell Survival, Cells, Cultured, Colloids, Dose-Response Relationship, Drug, Fibroblasts, Molybdenum, Oxides, Particle Size, Spectrum Analysis, Raman, Structure-Activity Relationship, Surface Properties, NIH 3T3 Cells, Cell Proliferation, Mice, Nanoparticles
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