Cited by 36Radiation Therapy and DosimetryEffects of Radiation ExposureNanoplatforms for cancer theranostics

Synergetic Influence of Bismuth Oxide Nanoparticles, Cisplatin and Baicalein-Rich Fraction on Reactive Oxygen Species Generation and Radiosensitization Effects for Clinical Radiotherapy Beams

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Talik Sisin, Noor Nabilah, Abdul Razak, Khairunisak, Zainal Abidin, Safri, Che Mat, Nor Fazila, Abdullah, Reduan, Ab Rashid, Raizulnasuha, Khairil Anuar, Muhammad Afiq, Rahman, Wan Nordiana·2020·International Journal of Nanomedicine

Abstract

PURPOSE: This study aimed to quantify synergetic effects induced by bismuth oxide nanoparticles (BiONPs), cisplatin (Cis) and baicalein-rich fraction (BRF) natural-based agent on the reactive oxygen species (ROS) generation and radiosensitization effects under irradiation of clinical radiotherapy beams of photon, electron and HDR-brachytherapy. The combined therapeutic responses of each compound and clinical radiotherapy beam were evaluated on breast cancer and normal fibroblast cell line. METHODS: In this study, individual BiONPs, Cis, and BRF, as well as combinations of BiONPs-Cis (BC), BiONPs-BRF (BB) and BiONPs-Cis-BRF (BCB) were treated to the cells before irradiation using HDR brachytherapy with 0.38 MeV iridium-192 source, 6 MV photon beam and 6 MeV electron beam. The individual or synergetic effects from the application of the treatment components during the radiotherapy were elucidated by quantifying the ROS generation and radiosensitization effects on MCF-7 and MDA-MB-231 breast cancer cell lines as well as NIH/3T3 normal cell line. RESULTS: The ROS generated in the presence of Cis stimulated the most substantial amount of ROS compared to the BiONPs and BRF. Meanwhile, the combination of the components had induced the higher ROS levels for photon beam than the brachytherapy and electron beam. The highest ROS enhancement relative to the control is attributable to the presence of BC combination in MDA-MB-231 cells, in comparison to the BB and BCB combinations. The radiosensitization effects which were quantified using the sensitization enhancement ratio (SER) indicate the highest value by BC in MCF-7 cells, followed by BCB and BB treatment. The radiosensitization effects are found to be more prominent for brachytherapy in comparison to photon and electron beam. CONCLUSION: The BiONPs, Cis and BRF are the potential radiosensitizers that could improve the efficiency of radiotherapy to eradicate the cancer cells. The combination of these potent radiosensitizers might produce multiple effects when applied in radiotherapy. The BC combination is found to have the highest SER, followed by the BCB combination. This study is also the first to investigate the effect of BRF in combination with BiONPs (BB) and BC (BCB) treatments.

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Authors
Talik Sisin, N. N., Abdul Razak, K., Zainal Abidin, S., Che Mat, N. F., Abdullah, R., Ab Rashid, R., Khairil Anuar, M. A., & Rahman, W. N.
Affiliations
Universiti Sains Malaysia (MY)
Corresponding author
Rahman, Wan Nordiana
Venue
International Journal of Nanomedicine
Published
2020-10
Volume
Volume 15
Pages
7805-7823
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W3091985370
Cited by
36
References
123
Citations by year
2026: 6 · 2025: 8 · 2024: 5 · 2023: 5 · 2022: 9 · 2021: 3
FWCI
2.39
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords
bismuth oxide nanoparticles, cisplatin, radiosensitization, reactive oxygen species, radiosensitizers, breast cancer, high-dose-rate brachytherapy, photon beams, electron beam, combination therapy, MCF-7 cells, MDA-MB-231 cells, normal fibroblasts, iridium-192
MeSH terms
Animals, Bismuth, Brachytherapy, Cisplatin, Drug Synergism, Female, Humans, Iridium Radioisotopes, Radiation-Sensitizing Agents, Reactive Oxygen Species, NIH 3T3 Cells, Flavanones, Mice, Nanoparticles, MCF-7 Cells

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