Expression systems for therapeutic glycoprotein production

Different gene amplification systems developed and used in CHO cells allow high titer yields and good specific productivity.

“Moreover, different gene amplification systems have been developed and used in CHO cells, which allow for high titer yields and good specific productivity (Durocher and Butler, 2009;Kim et al., 2012;Lai et al., 2013).”

Among mammalian-based expression systems, CHO cells are by far the most commonly used cell line.

“Among the mammalian-based expression systems, CHO cells is by far, the most commonly used cell line.”

CHO cells are involved in the production of over 70% of recombinant biopharmaceutical proteins, most of them monoclonal antibodies.

“It is involved in the production of over 70% of recombinant biopharmaceutical proteins, most of them being monoclonal antibodies (mAbs) (Durocher and Butler, 2009;Kim et al., 2012;Butler and Spearman, 2014).”

Transgenic animals often produce different glycosylation patterns compared to native human proteins.

“As with other mammalian expression systems, transgenic animals often produce different glycosylation pattern compared to native human proteins (Durocher and Butler, 2009).”

Only two marketed recombinant glycoproteins are currently manufactured in BHK cells, Factor VIIa and Factor VIII.

“Only two marketed recombinant glycoproteins are currently manufactured in these cells, Factor VIIa (NovoSeven ® ) and Factor VIII (Kogenate ® and Kovaltry ® ), which are other clotting factors (Durocher and Butler, 2009;Dumont et al., 2015).”

BHK cells are mostly used for the production of vaccines.

“Baby Hamster Kidney (BHK) cells are mostly been used for the production of vaccines (Durocher and Butler, 2009).”

Successful apoptosis inhibition was also achieved by overexpression of other related anti-apoptotic proteins.

“Successful apoptosis inhibition was also achieved by overexpression of other related anti-apoptotic proteins (Durocher and Butler, 2009;Bandaranayake and Almo, 2014).”

Addition of sodium butyrate (NaB) and valproic acid (VPA) has resulted in increased productivity in numerous studies, especially in CHO cells.

“Addition of these chemicals has resulted in increased productivity in numerous studies, especially in CHO cells (Durocher and Butler, 2009;Yang et al., 2014).”

CHO Lec13 cells and rat hybridoma YB2/0 cells were employed, the former for its impaired synthesis of GDP-fucose and the latter for its reduced expression level of the fucosyltransferase FUT8.

“As early strategies, CHO Lec13 cells and rat hybridoma YB2/0 cells were employed, the former for its impaired synthesis of GDP-fucose and the latter for its reduced expression level of the fucosyltransferase FUT8 (Durocher and Butler, 2009).”