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Beyond directed evolution—semi-rational protein engineering and design
Open accessAbstract
Over the last two decades, directed evolution has transformed the field of protein engineering. The advances in understanding protein structure and function, in no insignificant part a result of directed evolution studies, are increasingly empowering scientists and engineers to device more effective methods for manipulating and tailoring biocatalysts. Abandoning large combinatorial libraries, the focus has shifted to small, functionally-rich libraries and rational design. A critical component to the success of these emerging engineering strategies are computational tools for the evaluation of protein sequence datasets and the analysis of conformational variations of amino acids in proteins. Highlighting the opportunities and limitations of such approaches, this review focuses on recent engineering and design examples that require screening or selection of small libraries.
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Cited by
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Recent advances in (therapeutic protein) drug development
Lagassé, H. A. D., Alexaki, A., Simhadri, V. L., Katagiri, N. H., Jankowski, W., Sauna, Z. E., & Kimchi-Sarfaty, C. (2017) · F1000Research · doi:10.12688/f1000research.9970.1 · Cited by 481
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Biswas, S., Khimulya, G., Alley, E. C., Esvelt, K. M., & Church, G. M. (2021) · Nature Methods · doi:10.1038/s41592-021-01100-y · Cited by 422
Porebski, B. T., & Buckle, A. M. (2016) · Protein Engineering Design and Selection · doi:10.1093/protein/gzw015 · Cited by 215
Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution
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Metadata
- Authors
- Lutz, S.
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 21
- Issue
- 6
- Pages
- 734-743
- ISSN
- 0958-1669 (ISSN-L)
- OpenAlex
- W2085472819
- Cited by
- 528
- References
- 0
- FWCI
- 1.13
- Type
- journal article
- Language
- en
- Open access
- green
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