The emerging CHO systems biology era: harnessing the ‘omics revolution for biotechnology
Abstract
Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently sequenced. Now, the CHO systems biology era is underway. Critical 'omics data sets, including proteomics, transcriptomics, metabolomics, fluxomics, and glycomics, are emerging, allowing the elucidation of the molecular basis of CHO cell physiology. The incorporation of these data sets into mathematical models that describe CHO phenotypes will provide crucial biotechnology insights. As 'omics technologies and computational systems biology mature, genome-scale approaches will lead to major innovations in cell line development and metabolic engineering, thereby improving protein production and bioprocessing.
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The biopharmaceutical industry has entered the omics era, where systems biology is a central concept.
“The biopharmaceutical industry has entered the “omics” era, where systems biology is a central concept (Kildegaard et al., 2013).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Kildegaard et al., 2013
Cites
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- Authors
- Kildegaard, H. F., Baycin-Hizal, D., Lewis, N. E., & Betenbaugh, M. J.
- Affiliations
- Novo Nordisk Foundation (DK)
- Brigham Young University (US)
- Johns Hopkins University (US)
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 24
- Issue
- 6
- Pages
- 1102-1107
- ISSN
- 0958-1669 (ISSN-L)
- OpenAlex
- W2049762689
- Cited by
- 165
- References
- 3
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 10 · 2023: 2 · 2022: 14 · 2021: 16 · 2020: 14 · 2019: 9 · 2018: 15 · 2017: 17 · 2016: 15 · 2015: 21 · 2014: 19 · 2013: 5
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Funders
- Novo Nordisk Foundation
- US National Science Foundation (CBET-1067312)
- US Dept of Energy (DE-FG02-03ER6344)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- CHO cells, systems biology, biopharmaceutical manufacturing, proteomics, transcriptomics, metabolomics, fluxomics, glycomics, cell line development, metabolic engineering, recombinant protein expression, bioprocessing
- MeSH terms
- Animals, Biotechnology, Cricetulus, Humans, Models, Biological, Recombinant Proteins, CHO Cells, Genome, Proteomics, Systems Biology, Glycomics, Metabolomics, Transcriptome, Metabolic Engineering
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