ATP Consumption by Mammalian Rod Photoreceptors in Darkness and in Light

Abstract

Summary Why do vertebrates use rods and cones that hyperpolarize, when in insect eyes a single depolarizing photoreceptor can function at all light levels [1, 2]? We answer this question at least in part with a comprehensive assessment of ATP consumption for mammalian rods from voltages and currents and recently published physiological and biochemical data. In darkness, rods consume 108 ATP s−1, about the same as Drosophila photoreceptors [3]. Ion fluxes associated with phototransduction and synaptic transmission dominate; as in CNS [4], the contribution of enzymes of the second-messenger cascade is surprisingly small. Suppression of rod responses in daylight closes light-gated channels and reduces total energy consumption by >75%, but in Drosophila light opens channels and increases consumption five-fold [5]. Rods therefore provide an energy-efficient mechanism for high-sensitivity vision in dim light not present in rhabdomeric photoreceptors. Rods are metabolically less “costly” than cones, since cones do not saturate in bright light [6, 7] and use more ATP s−1 for transducin activation [8] and rhodopsin phosphorylation [9]. This helps to explain why the vertebrate retina is duplex, and why some diurnal animals like primates have a small number of cones, concentrated in a region of high acuity.