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Cited by 423Microtubule and mitosis dynamicsCellular transport and secretionstochastic dynamics and bifurcation

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Motor Coordination via a Tug-of-War Mechanism Drives Bidirectional Vesicle Transport

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Hendricks, Adam G., Perlson, Eran, Ross, Jennifer L., Schroeder, Harry W., Tokito, Mariko, Holzbaur, Erika L.F.·2010·Current Biology
DOI 10.1016/j.cub.2010.02.058PMC PMC2908734
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Abstract

The microtubule motors kinesin and dynein function collectively to drive vesicular transport. High-resolution tracking of vesicle motility in the cell indicates that transport is often bidirectional, characterized by frequent directional changes. However, the mechanisms coordinating the collective activities of oppositely oriented motors bound to the same cargo are not well understood. To examine motor coordination, we purified neuronal transport vesicles and analyzed their motility via automated particle tracking with nanometer resolution. The motility of purified vesicles reconstituted in vitro closely models the movement of LysoTracker-positive vesicles in primary neurons, where processive bidirectional motility is interrupted with frequent directional switches, diffusional movement, and pauses. Quantitative analysis indicates that vesicles copurify with a low number of stably bound motors: one to five dynein and one to four kinesin motors. These observations compare well to predictions from a stochastic tug-of-war model, where transport is driven by the force-dependent kinetics of teams of opposing motors in the absence of external regulation. Together, these observations indicate that vesicles move robustly with a small complement of tightly bound motors and suggest an efficient regulatory scheme for bidirectional motility where small changes in the number of engaged motors manifest in large changes in the motility of cargo.

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Metadata

Authors
Hendricks, A. G., Perlson, E., Ross, J. L., Schroeder, H. W., Tokito, M., & Holzbaur, E. L. F.
Affiliations
  • University of Pennsylvania (US)
  • University of Massachusetts Amherst (US)
Corresponding author
Holzbaur, Erika L.F.
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2010-04
Volume
20
Issue
8
Pages
697-702
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/j.cub.2010.02.058
PMC
PMC2908734
OpenAlex
W1972398314
Cited by
423
References
29
Citations by year
2026: 10 · 2025: 21 · 2024: 16 · 2023: 19 · 2022: 13 · 2021: 24 · 2020: 26 · 2019: 28 · 2018: 36 · 2017: 31 · 2016: 30 · 2015: 20 · 2014: 36 · 2013: 27 · 2012: 38
FWCI
15.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation
  • National Institutes of Health (1f32gm075754-01)
  • National Institute of General Medical Sciences (GM-48661, P01GM087253, GM087253, F32 GM075754, R01GM048661, GM071339, 1F32GM075754, T32GM071339)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Physical Sciences › Physics and Astronomy › Statistical and Nonlinear Physics › stochastic dynamics and bifurcation
Keywords
kinesin, dynein, motor coordination, tug-of-war model, directional switching, processive motility, in vitro reconstitution, particle tracking
MeSH terms
Dynactin Complex, Animals, Biological Transport, Dyneins, Mice, Transgenic, Microtubule-Associated Proteins, Microtubules, Neurons, Recombinant Fusion Proteins, Stress, Mechanical, Kinesins, Transport Vesicles, Mice

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