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Real-Time Visualization of Neuronal Activity during Perception
Open accessAbstract
To understand how the brain perceives the external world, it is desirable to observe neuronal activity in the brain in real time during perception. The zebrafish is a suitable model animal for fluorescence imaging studies to visualize neuronal activity because its body is transparent through the embryonic and larval stages. Imaging studies have been carried out to monitor neuronal activity in the larval spinal cord and brain using Ca(2+) indicator dyes and DNA-encoded Ca(2+) indicators, such as Cameleon, GFP-aequorin, and GCaMPs. However, temporal and spatial resolution and sensitivity of these tools are still limited, and imaging of brain activity during perception of a natural object has not yet been demonstrated. Here we demonstrate visualization of neuronal activity in the optic tectum of larval zebrafish by genetically expressing the new version of GCaMP. First, we demonstrate Ca(2+) transients in the tectum evoked by a moving spot on a display and identify direction-selective neurons. Second, we show tectal activity during perception of a natural object, a swimming paramecium, revealing a functional visuotopic map. Finally, we image the tectal responses of a free-swimming larval fish to a paramecium and thereby correlate neuronal activity in the brain with prey capture behavior.
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“Like in other vertebrates, the superficial layers of the teleostean optic tectum receive a topographically ordered primary retinal projection that forms a retinotopic visual map over the tectal surface”
From Spatial Cognition in Teleost Fish: Strategies and Mechanisms (2021) · cites this paper as [136]
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Metadata
- Authors
- Muto, A., Ohkura, M., Abe, G., Nakai, J., & Kawakami, K.
- Affiliations
- National Institute of Genetics (JP)
- Saitama University (JP)
- Corresponding author
- Nakai, Junichi
- Kawakami, Koichi
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2013-02
- Volume
- 23
- Issue
- 4
- Pages
- 307-311
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W1971978102
- Cited by
- 248
- References
- 24
- Citations by year
- 2026: 7 · 2025: 15 · 2024: 15 · 2023: 18 · 2022: 13 · 2021: 11 · 2020: 22 · 2019: 21 · 2018: 15 · 2017: 31 · 2016: 26 · 2015: 20 · 2014: 16 · 2013: 18
- FWCI
- 12.29
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Mitsubishi Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- neuronal activity imaging, zebrafish, zebrafish larvae, GCaMP, calcium indicator dyes, optic tectum, direction-selective neurons, visuotopic map, Paramecium, prey capture, visual perception
- MeSH terms
- Animals, Behavior, Animal, Brain, Optic Nerve, Perception, Spinal Cord, Superior Colliculi, Vision, Ocular, Zebrafish, Animals, Genetically Modified, Green Fluorescent Proteins, Functional Neuroimaging
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