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Cited by 41Olfactory and Sensory Function StudiesIon Channels and ReceptorsBiochemical Analysis and Sensing Techniques

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Antagonistic interactions between odorants alter human odor perception

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Fukutani, Yosuke, Abe, Masashi, Saito, Haruka, Eguchi, Ryo, Tazawa, Toshiaki, de March, Claire A., Yohda, Masafumi, Matsunami, Hiroaki·2023·Current Biology
DOI 10.1016/j.cub.2023.04.072PMC PMC10394640

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Abstract

Abstract The olfactory system detects a vast number of odorants using hundreds of olfactory receptors (ORs), the largest group of the G protein-coupled receptor (GPCR) superfamily. Each OR is activated by specific odorous ligands. Like other GPCRs, activation of ORs may be blocked through antagonism. Recent reports highlight widespread antagonisms in odor mixtures influencing olfactory neuron activities. However, it is unclear if and how these antagonisms influence perception of odor mixtures. Here we show that odorant antagonisms at the receptor level alter odor perception. Using a large-scale heterologous expression, we first identified a set of human ORs that are activated by methanethiol and hydrogen sulfide, two extremely potent volatile sulfur malodors. We then screened odorants that block activation of these ORs and identified a set of antagonists, including β-ionone. Finally, human sensory evaluation revealed that odor intensity and unpleasantness of methanethiol were decreased by β-ionone. Odor intensity of β-ionone itself is not correlated with the degree of suppression of malodor sensation. Suppression was also not observed when methanethiol and β-ionone were simultaneously introduced to different nostrils. Together, our data supports the model that odor sensation is altered through antagonistic interactions at the level of the ORs.

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Metadata

Authors
Fukutani, Y., Abe, M., Saito, H., Eguchi, R., Tazawa, T., de March, C. A., Yohda, M., & Matsunami, H.
Affiliations
  • Institute of Global Innovation Research (Tokyo University of Agriculture and Technology) (JP)
  • Tokyo University of Agriculture and Technology (JP)
  • S.T. Corporation (Japan) (JP)
  • Centre National de la Recherche Scientifique (FR)
  • Duke University (US)
Corresponding author
  • Fukutani, Yosuke
  • Yohda, Masafumi
  • Matsunami, Hiroaki
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2023-06
Volume
33
Issue
11
Pages
2235-2245.e4
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/j.cub.2023.04.072
PMC
PMC10394640
OpenAlex
W4377292694
Cited by
41
References
2
Citations by year
2026: 23 · 2025: 12 · 2024: 4 · 2023: 2
FWCI
7.59
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • HAL (submittedVersion, other-oa)
Funders
  • Japan Society for the Promotion of Science (18K14060, 20H02532, 20K15745)
  • Japan Science and Technology Agency
  • Kao Corporation
  • Program on Open Innovation Platform with Enterprises Research Institute and Academia (JPMJOP1833)
  • Japan Science and Technology Agency Strategic Basic Research Programs ACT-X (JPMJAX201C)
  • National Institutes of Health (DC014423, DC016224, K99DC018333)
  • National Science Foundation (1555919)
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
Keywords
olfactory receptors, olfaction, odor mixtures, methanethiol, hydrogen sulfide, β-ionone, volatile sulfur compounds, odor intensity, G protein-coupled receptors, heterologous expression
MeSH terms
Humans, Odorants, Perception, Smell, Sulfhydryl Compounds, Olfactory Receptor Neurons, Receptors, Odorant, Norisoprenoids, Olfactory Perception

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