Interferon-Beta Induces Major Histocompatibility Complex of Class I (MHC-I) Expression and a Proinflammatory Phenotype in Cultivated Human Astrocytes
Abstract
Major histocompatibility complex class I (MHC-I) has been implicated in several types of neuroplasticity phenomena. Interferon beta-1b (IFN-β) increases MHC-I expression by motoneurons after sciatic nerve crush in mice, improving axonal growth and functional recovery. Additionally, IFN-β induces glial hypertrophy associated with upregulation of glial fibrillary acidic protein (GFAP) and MHC-I in murine astrocytes in vitro. As knowledge about MHC-I and its role in synaptic plasticity in human astrocytes (HAs) is scarce, we investigated these aspects in mature HAs obtained from the neocortex of patients undergoing surgery due to hippocampal sclerosis. Cells were exposed to media in the absence (0 IU/ml) or presence of IFN-β for 5 days (500 IU/ml). Beta-2 microglobulin (β2m), a component of the MHC-I, GFAP and vimentin proteins, was quantified by flow cytometry (FC) and increased by 100%, 60% and 46%, respectively, after IFN-β exposure. We also performed qRT-PCR gene expression analyses for β2m, GFAP, vimentin, and pro- and anti-inflammatory cytokines. Our data showed that IFN-β-treated astrocytes displayed β2m and GFAP gene upregulation. Additionally, they presented a proinflammatory profile with increase in the IL-6 and IL-1β genes and a tendency to upregulate TNF-α. Moreover, we evaluated the effect of HAs conditioned medium (CM) on the formation/maintenance of neurites/synapses by the PC12 lineage. Synaptophysin protein expression was quantified by FC. The CM of IFN-β-activa…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Many studies have cultured primary human and rodent astrocytes in media with high concentrations of glucose.
“Many studies have cultured primary human and rodent astrocytes in media with high concentrations of glucose (e.g., [20,21,35]).”
From Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation (2023) · cites this paper as [35]
Cites
Neurotoxic reactive astrocytes are induced by activated microglia
Liddelow, S. A., Guttenplan, K. A., Clarke, L. E., Bennett, F. C., Bohlen, C. J., Schirmer, L., Bennett, M. L., Münch, A. E., Chung, W.-S., Peterson, T. C., et al. (2017) · Nature · doi:10.1038/nature21029 · Cited by 7,971 · see citing works
Reactive astrocyte nomenclature, definitions, and future directions
Escartin, C., Galea, E., Lakatos, A., O’Callaghan, J. P., Petzold, G. C., Serrano-Pozo, A., Steinhäuser, C., Volterra, A., Carmignoto, G., Agarwal, A., et al. (2021) · Nature Neuroscience · doi:10.1038/s41593-020-00783-4 · Cited by 2,533 · see citing works
Functional requirement for class I MHC in CNS development and plasticity
Huh, G. S., Boulanger, L. M., Du, H., Riquelme, P. A., Brotz, T. M., & Shatz, C. J. (2000) · Science · doi:10.1126/science.290.5499.2155 · Cited by 828 · see citing works
Neurons produce type I interferon during viral encephalitis
Delhaye, S., Paul, S., Blakqori, G., Minet, M., Weber, F., Staeheli, P., & Michiels, T. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602460103 · Cited by 266 · see citing works
Major histocompatibility complex class I proteins in brain development and plasticity
Elmer, B. M., & McAllister, A. K. (2012) · Trends in Neurosciences · doi:10.1016/j.tins.2012.08.001 · Cited by 196 · see citing works
Hyvärinen, T., Hagman, S., Ristola, M., Sukki, L., Veijula, K., Kreutzer, J., Kallio, P., & Narkilahti, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-53414-9 · Cited by 168 · see citing works
Properties of human astrocytes and NG2 glia
Bedner, P., Jabs, R., & Steinhäuser, C. (2020) · Glia · doi:10.1002/glia.23725 · Cited by 71 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Silva, E. A., Dalla Costa, A. P., Ruas, J. S., Siqueira-Santos, E. S., Francisco, A., & Castilho, R. F. (2023) · Cells · doi:10.3390/cells12050683 · Cited by 5 · see citing works
- Authors
- Ignarro, R. S., Bombeiro, A. L., Chiarotto, G. B., Cartarozzi, L. P., Coser, L. D. O., Ghizoni, E., Tedeschi, H., Cendes, F., Lopes-Cendes, I., Rogerio, F., & Oliveira, A. L. R. D.
- Affiliations
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Corresponding author
- Oliveira, Alexandre Leite Rodrigues de
- ORCID
- Venue
- Differentiation
- Publisher
- Elsevier BV
- Published
- 2022-11
- Volume
- 128
- Pages
- 43-56
- ISSN
- 0301-4681 (ISSN-L)
- OpenAlex
- W4306318690
- Cited by
- 13
- References
- 7
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 1 · 2022: 1
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Differentiation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2018/05006\u20130, 2013/07559\u20133, 2017/03619\u20132, 2285/17)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- interferon-beta, MHC class I, human astrocytes, beta2-microglobulin, glial fibrillary acidic protein, vimentin, astrocyte activation, inflammatory cytokines, interleukin-6, IL-1β, TNF-α, conditioned medium, synaptophysin, neurite outgrowth, hippocampal sclerosis, neuroplasticity
- MeSH terms
- Animals, Astrocytes, Humans, Major Histocompatibility Complex, Phenotype, Vimentin, Histocompatibility Antigens Class I, Synaptophysin, Interferon-beta, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
