Cell banking for pharmaceutical research

Wrigley, Jonathan D.; McCall, Eileen J.; Bannaghan, Claire L.; Liggins, Laura; Kendrick, Clare; Griffen, Alison; Hicks, Ryan; Fröderberg-Roth, Linda

doi:10.1016/j.drudis.2014.05.006

Cited by 18Viral Infectious Diseases and Gene Expression in InsectsAnimal testing and alternativesMicrobial infections and disease research

Cell banking for pharmaceutical research

Wrigley, Jonathan D., McCall, Eileen J., Bannaghan, Claire L., Liggins, Laura, Kendrick, Clare, Griffen, Alison, Hicks, Ryan, Fröderberg-Roth, Linda·2014·Drug Discovery Today

DOI 10.1016/j.drudis.2014.05.006

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Abstract

The provision of high-quality eukaryotic cells through robust cell banking processes is essential for the progression of drug discovery projects throughout the pharmaceutical research process. Numerous models exist to meet this aim, and this review describes many of the underlying principles, challenges and opportunities as well as detailing how these have been addressed within AstraZeneca. Crucial aspects discussed include cell line acquisition, cell bank generation, cryopreservation, storage, tracking and distribution. Because quality assurance underpins much of the process, quality control (QC) testing including mycoplasma screening and cell line authentication are also discussed in detail. Furthermore, because many of the underlying principles of cell banking are applicable in non-pharmaceutical settings, it is hoped that this review will prove a useful resource across the wider scientific community.

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STR profiling is well established with standard protocols published by the American National Standards Institute, relatively inexpensive, and flexible.
“Adopted by pharmaceutical companies and academia for the characterization of primary and immortalized cells [11,19], STR profiling is well established with standard protocols published by the American National Standards Institute (”
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Authors: Wrigley, J. D., McCall, E. J., Bannaghan, C. L., Liggins, L., Kendrick, C., Griffen, A., Hicks, R., & Fröderberg-Roth, L.
Affiliations: AstraZeneca (United Kingdom) (GB)
AstraZeneca (Sweden) (SE)
Corresponding author: Wrigley, Jonathan D.
ORCID: Wrigley, Jonathan D.
Hicks, Ryan
Venue: Drug Discovery Today
Publisher: Elsevier BV
Published: 2014-10
Volume: 19
Issue: 10
Pages: 1518-1529
ISSN: 1359-6446 (ISSN-L)
DOI: 10.1016/j.drudis.2014.05.006
OpenAlex: W2016439976
Cited by: 18
References: 42
Citations by year: 2025: 3 · 2024: 2 · 2023: 3 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 3
FWCI: 0.19
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Drug Discovery Today (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords: cell banking, cryopreservation, cell line authentication, quality control, drug discovery, AstraZeneca
MeSH terms: Cell Line, Humans, Specimen Handling, Biological Specimen Banks, Biomedical Research

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Cell banking for pharmaceutical research

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