Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip
Abstract
Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations. The present manuscript describes the setting up of a dielectrophoresis (DEP)-based lab-on-a-chip platform for the real-time study of Gap Junctional Intercellular Communication between osteosarcoma cells and the main cells accessible to their microenvironment. We conclude that using the DEParray technology for the GJIC assessment has several advantages comparing to current techniques. This methodology is less harmful for cells integrity; cells can be recovered after interaction to make further molecular analysis; it is possible to study GJIC in real time; we can promote cell interactions using up to five different populations. The setting up of this new methodology overcomes several difficulties to perform experiments for solving questions about GJIC process that we are not able to do with current technics.
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The dialogue between osteosarcoma cells and their microenvironment is crucial for tumour growth at the bone site and involves direct interactions among mesenchymal, vascular and immune cells, soluble factors such as chemokines and cytokines, and extracellular vesicles.
“The dialogue between osteosarcoma cells and their microenvironment is crucial for tumour growth at the bone site and is associated with direct interaction among mesenchymal, vascular and immune cells (depending on cell differentiation level) 45 , the interaction of cells with soluble factors such as chemokines and cytokines 46 , and the interaction of cells with extracellular vesicles 47 .”
From Osteosarcoma (2022) · cites this paper as [45]
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- Authors
- Tellez-Gabriel, M., Charrier, C., Brounais-Le Royer, B., Mullard, M., Brown, H. K., Verrecchia, F., & Heymann, D.
- Affiliations
- Inserm (FR)
- Corresponding author
- Heymann, Dominique
- Venue
- European Journal of Cell Biology
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 96
- Issue
- 2
- Pages
- 110-118
- ISSN
- 0171-9335 (ISSN-L)
- OpenAlex
- W2583814456
- Cited by
- 14
- References
- 34
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 3
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- European Journal of Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- HAL (submittedVersion)
- Funders
- Bone Cancer Research Trust (144681)
- Seventh Framework Programme (264817–BONE-NET)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- gap junctional intercellular communication, dielectrophoresis, lab-on-a-chip, DEPArray, osteosarcoma, bone tumors, tumor microenvironment, intercellular communication
- MeSH terms
- Bone Neoplasms, Cell Communication, Cell Differentiation, Humans, Osteoblasts, Osteosarcoma, Gap Junctions, Cell Line, Tumor, Electrophoresis, Microchip, Time-Lapse Imaging, Mesenchymal Stem Cells
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