Removal of pharmaceutical compounds in water and wastewater using fungal oxidoreductase enzymes
Open accessAbstract
Due to recalcitrance of some pharmaceutically active compounds (PhACs), conventional wastewater treatment is not able to remove them effectively. Therefore, their occurrence in surface water and potential environmental impact has raised serious global concern. Biological transformation of these contaminants using white-rot fungi (WRF) and their oxidoreductase enzymes has been proposed as a low cost and environmentally friendly solution for water treatment. The removal performance of PhACs by a fungal culture is dependent on several factors, such as fungal species, the secreted enzymes, molecular structure of target compounds, culture medium composition, etc. In recent 20 years, numerous researchers tried to elucidate the removal mechanisms and the effects of important operational parameters such as temperature and pH on the enzymatic treatment of PhACs. This review summarizes and analyzes the studies performed on PhACs removal from spiked pure water and real wastewaters using oxidoreductase enzymes and the data related to degradation efficiencies of the most studied compounds. The review also offers an insight into enzymes immobilization, fungal reactors, mediators, degradation mechanisms and transformation products (TPs) of PhACs. In brief, higher hydrophobicity and having electron-donating groups, such as amine and hydroxyl in molecular structure leads to more effective degradation of PhACs by fungal cultures. For recalcitrant compounds, using redox mediators, such as syringaldehyde increases the degradation efficiency, however they may cause toxicity in the effluent and deactivate the enzyme. Immobilization of enzymes on supports can enhance the performance of enzyme in terms of reusability and stability. However, the immobilization strategy should be carefully selected to reduce the cost and enable regeneration. Still, further studies are needed to elucidate the mechanisms involved in enzymatic degradation and the toxicity levels of TPs and also to optimize the whole treatment strategy to have economical and technical competitiveness.
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Extracellular enzymes produced by microalgae break down certain antibiotics into less toxic or non-toxic intermediates.
“Extracellular enzymes produced by microalgae break down certain antibiotics into less toxic or even non-toxic intermediates [164].”
From Microalgae-based wastewater treatment: Mechanisms, challenges, recent advances, and future prospects (2023) · cites this paper as [164]
Section 2.2 (page unavailable) reports degradation of the antibiotic ofloxacin by fungi and reduced toxicity in the treated medium. It does not attribute that process to extracellular enzymes produced by microalgae, nor establish that the resulting intermediates are non-toxic. (LLM, conf=0.85)
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“Extracellular enzymes produced by microalgae break down certain antibiotics into less toxic or even non-toxic intermediates [164].”
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Use of microalgae based technology for the removal of antibiotics from wastewater: A review
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Fluidized bed bioreactor for multiple environmental engineering solutions
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Electrochemical detection and removal of pharmaceuticals in waste waters
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Removal of two cytostatic drugs: bleomycin and vincristine by white-rot fungi – a sorption study
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Resource recovery and treatment of wastewaters using filamentous fungi
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Inhibition in multicopper oxidases: a critical review
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Treatment of wastewater containing pharmaceuticals: biological treatment
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Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment
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Towards oxidoreductase-based processes for the removal of antibiotics from wastewater
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- Authors
- Naghdi, M., Taheran, M., Brar, S. K., Kermanshahi-pour, A., Verma, M., & Surampalli, R. Y.
- Affiliations
- Institut National de la Recherche Scientifique (CA)
- Corresponding author
- Brar, Satinder Kaur
- Venue
- Environmental Pollution
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 234
- Pages
- 190-213
- ISSN
- 0269-7491 (ISSN-L)
- OpenAlex
- W2769826121
- Cited by
- 275
- References
- 218
- Citations by year
- 2026: 21 · 2025: 32 · 2024: 40 · 2023: 41 · 2022: 35 · 2021: 36 · 2020: 29 · 2019: 25 · 2018: 16
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Environmental Pollution (publishedVersion)
- PubMed (publishedVersion)
- EspaceINRS (National Institute for Scientific Research (Canada)) (acceptedVersion)
- Funders
- Ministère des relations internationales et de la Francophonie (122523)
- Natural Sciences and Engineering Research Council of Canada (355254)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Enzyme-mediated dye degradation
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Physical Sciences › Environmental Science › Water Science and Technology › Advanced oxidation water treatment
- Keywords
- pharmaceutical active compounds, white-rot fungi, oxidative enzymes, enzymatic degradation, wastewater treatment, water treatment, enzyme immobilization, redox mediators, syringaldehyde, degradation intermediates, degradation mechanism, molecular structure effects, enzyme reusability
- MeSH terms
- Basidiomycota, Biodegradation, Environmental, Pharmaceutical Preparations, Oxidoreductases, Waste Disposal, Fluid, Water Pollutants, Chemical, Water Purification, Biocatalysis, Wastewater
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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