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Aging impairs mitochondrial respiratory capacity in classical monocytes
Open accessAbstract
Aging is a critical healthcare concern, with age-related inflammation disposing individuals to a variety of diseases. Monocytes are affected by the aging process, with increased inflammation and impaired cellular functions such as phagocytosis. Mechanisms by which aging alters monocyte function are unknown, but recent research suggests that the balance of metabolic processes determine immune cell phenotype and function. Given the known association between aging and mitochondrial dysfunction in other tissues, we hypothesized that aging would impair mitochondrial function in monocytes. To test this, we isolated classical monocytes from young and older adults and tested mitochondrial function by a Seahorse assay. Aging reduced mitochondrial respiratory capacity and spare capacity in monocytes. Mitochondrial dysfunction is a potential mechanism by which aging alters monocyte phenotype and may impair inflammatory functions, especially in low-glucose environments where oxidative metabolism is necessary to meet energy demands.
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“It has been observed that respiratory capacity steadily declines with age in CD14 + monocytes as a consequence of mitochondrial dysfunction (144).”
From The impact of ageing on monocytes and macrophages (2021) · cites this paper as [144]
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Pence, B. D. (2017) · doi:10.17504/protocols.io.kwtcxen · Cited by 1 · cited 2×
“Detailed step-by-step protocols and required supplies for major assays described here (monocyte isolation, Seahorse assay, flow cytometry) are available on protocols.io (Pence 2017b, 2017c, 2017d).”
Self-citationFlow Cytometry for Monocyte Phenotype and Mitochondrial Mass v1Preprint
Pence, B. D. (2017) · doi:10.17504/protocols.io.kw8cxhw
Self-citationCell Mito Stress Test for Monocyte Mitochondrial Function v1Preprint
Pence, B. D. (2017) · doi:10.17504/protocols.io.kw7cxhn · cited 2×
“Detailed step-by-step protocols and required supplies for major assays described here (monocyte isolation, Seahorse assay, flow cytometry) are available on protocols.io (Pence 2017b, 2017c, 2017d).”
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The integration of inflammaging in age-related diseases
Fülöp, T., Witkowski, J. M., Olivieri, F., & Larbi, A. (2018) · Seminars in Immunology · doi:10.1016/j.smim.2018.09.003 · Cited by 368
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De Maeyer, R. P. H., & Chambers, E. S. (2021) · Immunology Letters · doi:10.1016/j.imlet.2020.12.003 · Cited by 275
“It has been observed that respiratory capacity steadily 561 declines with age in CD14 monocytes as a consequence of mitochondrial dysfunction (144).”
BackgroundMitochondrial function in immune cells in health and disease
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Background
Metadata
- Authors
- Pence, B. D., & Yarbro, J. R.
- Affiliations
- University of Memphis (US)
- Corresponding author
- Pence, Brandt D.
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2018-07
- Volume
- 108
- Pages
- 112-117
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W2798053566
- Cited by
- 120
- References
- 40
- Citations by year
- 2026: 11 · 2025: 8 · 2024: 19 · 2023: 11 · 2022: 17 · 2021: 21 · 2020: 18 · 2019: 10 · 2018: 5
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Experimental Gerontology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- University of Memphis
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- aging, classical monocytes, mitochondrial respiratory capacity, mitochondrial dysfunction, spare respiratory capacity, age-related inflammation, monocyte phenotype, oxidative metabolism, glucose metabolism, immune cell function
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Aging, Female, Flow Cytometry, Humans, Immunity, Innate, Inflammation, Male, Middle Aged, Mitochondria, Monocytes, Phagocytosis, Cell Respiration, Young Adult
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