Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 16

The DOI matches its Crossref record; 103 of the 103 references it cites were found in a registry or an index.

Ryanodine receptor inhibitor dantrolene reduces hypoxic-ischemic brain injury in neonatal mice

Ovcjak, Andrea, Xiao, Aijiao, Kim, Ji-Sun, Xu, Baofeng, Szeto, Vivian, Turlova, Ekaterina, Abussaud, Ahmed, Chen, Nai-hong, Miller, Steven P., Sun, Hong-Shuo, Feng, Zhong-Ping·2022·Experimental Neurology
DOI 10.1016/j.expneurol.2022.113985
Open this paper in CoChat — free account

Abstract

Ryanodine receptors (RyR) located on the membrane of the endoplasmic reticulum (ER), are a potent regulator of intracellular calcium levels upon activation. Dysregulated Ca 2+ homeostasis is characteristic of hypoxic-ischemic (HI) brain injury and ultimately leads to neurodegeneration. RyRs have thereby been implicated in the Ca 2+ imbalance that occurs during and after HI. In this study, we investigated the effects of RyR antagonist, dantrolene, on HI brain injury in neonatal mice. We found that administration of dantrolene (i.p.) on postnatal day 7 mice reduced the infarction volume and morphological damage induced by HI, and improved functional recovery as assessed by neurobehavioral testing. The neuroprotective effect of dantrolene was further demonstrated in neuronal cell culture in vitro, where dantrolene significantly reduced oxygen-glucose deprivation (OGD)-induced cell death. Fura-2 calcium imaging confirmed that dantrolene reduced the intracellular calcium level in cultured cortical neurons in vitro. Finally, Western blot analysis showed that dantrolene treatment reduced cleaved caspase-3 and -9 apoptotic proteins, and elevated pro-survival protein kinase C (PKC) protein levels. Taken together, our results demonstrate that dantrolene exerts neuroprotective effects against neonatal HI brain injury. This suggests that RyRs play a role in mediating the ionic imbalance induced by HI and therefore represent a potential target for drug development.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.expneurol.2022.113985

Cites

103 of 103 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

1 retracted

Show all 103 references

Research works printed but not linked: 4

  1. Gursoy, 2020, Investigation of the possible protective effects of ketamine and Dantrolene on the hippocampal apoptosis and spatial learning in rats exposed to repeated electroconvulsive seizures as a model of status epilepticus, Turk. Neurosurg., 30, 871
  2. Hua Wang, 2002, Roles of protein kinase C and inositol(1,4,5)-trisphosphate in the pathogenesis of hypoxic- ischemic brain injury in neonatal rats - PubMed, Chin. Med. J., 116, 829
  3. Johnston, 1995, Hypoxic and ischemic central nervous system disorders in infants and Childrenin infants and children - PubMed, Adv. Pediatr. Infect. Dis., 42, 1
  4. Mennerich, 2014, Direct phosphorylation events involved in HIF-alpha regulation: the role of GSK-3beta, Hypoxia, 2, 45

Cited by

See all 16

Metadata

Authors
Ovcjak, A., Xiao, A., Kim, J.-S., Xu, B., Szeto, V., Turlova, E., Abussaud, A., Chen, N.-H., Miller, S. P., Sun, H.-S., & Feng, Z.-P.
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
2022-05
Volume
351
Pages
113985
ISSN
0014-4886
DOI
10.1016/j.expneurol.2022.113985
Cited by
16
References
103
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Ryanodine receptor inhibitor dantrolene reduces hypoxic-ischemic brain injury in neonatal mice. https://app.cochat.ai/papers/10.1016/j.expneurol.2022.113985
The paper
Ovcjak, A., Xiao, A., Kim, J.-S., Xu, B., Szeto, V., Turlova, E., Abussaud, A., Chen, N.-H., Miller, S. P., Sun, H.-S., & Feng, Z.-P. (2022). Ryanodine receptor inhibitor dantrolene reduces hypoxic-ischemic brain injury in neonatal mice. Experimental Neurology. https://doi.org/10.1016/j.expneurol.2022.113985

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.