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Detoxification of patulin and ochratoxin A, two abundant mycotoxins, by lactic acid bacteria
Abstract
Aim of the present study was to investigate the detoxification of two abundant mycotoxins, namely ochratoxin A (OTA) and patulin (PAT) which are frequently found in human foods, by lactic acid bacteria. The removal of the two mycotoxins from liquid medium by thirty different LAB strains was analyzed in a screening trial by the use of HPLC coupled with UV- or fluorescence detection. Two highly effective strains were identified; Lactobacillus acidophilus VM 20 caused a decrease of OTA by > or = 95% and Bifidobacterium animalis VM 12 reduced PAT levels by 80%. Subsequently experiments showed that the binding of these compounds depends on different parameters, i.e. the concentration of toxins, the cell density, the pH-value and on the viability of the bacteria. To proof that the decrease of the toxins by LAB from liquid medium results in a reduction of their toxic properties, micronucleus (MCN) assays were conducted with a human derived hepatoma cell line (HepG2). Indeed, a substantial decrease (39-59%) of OTA and PAT induced MCN formation was observed with the most effective strains detected in the chemical analyses. Furthermore, also the inhibition of the cell division rates by the toxins was significantly reduced. These findings indicate that certain LAB strains are able to detoxify the two toxins and may be useful to protect humans and/or animals against the adverse health effects of these compounds.
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“LAB strains isolated from plant environments are able to eliminate mycotoxins, such as OTA, patulin and AFs, and fusarium toxins like deoxynivalenol, zearalenone and fumonisins (Fuchs et al. 2008; Niderkorn et al. 2007).”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Fuchs et al. 2008
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Metadata
- Authors
- Fuchs, S., Sontag, G., Stidl, R., Ehrlich, V., Kundi, M., & Knasmüller, S.
- Affiliations
- University of Vienna (AT)
- Medical University of Vienna (AT)
- Corresponding author
- Ehrlich, V.
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2008-04
- Volume
- 46
- Issue
- 4
- Pages
- 1398-1407
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2016865034
- Cited by
- 345
- References
- 75
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 16 · 2023: 17 · 2022: 28 · 2021: 26 · 2020: 22 · 2019: 22 · 2018: 29 · 2017: 24 · 2016: 15 · 2015: 17 · 2014: 27 · 2013: 14 · 2012: 20
- FWCI
- 11.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytase and its Applications
- Keywords
- mycotoxin detoxification, patulin, ochratoxin A, lactic acid bacteria, Lactobacillus acidophilus, Bifidobacterium animalis, toxin binding, bacterial viability, micronucleus formation, HepG2 cells, cell division inhibition, food safety
- MeSH terms
- Bifidobacterium, Cell Division, Cell Line, Chromatography, High Pressure Liquid, Culture Media, Humans, Hydrogen-Ion Concentration, Lactobacillus acidophilus, Inactivation, Metabolic, Ochratoxins, Patulin, Spectrophotometry, Ultraviolet, Micronucleus Tests, Chromosome Breakage
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