Aluminum-induced molecular neurodegeneration: The protective role of genistein and chickpea extract
Abstract
Neurotoxicity of Al is well established and linked to oxidative damage and neurodegeneration. This study investigated the protective role of genistein (Gen) and chickpea extract (CPE) against AlCl 3-induced neurodegeneration. HPLC analysis revealed that biochanin A-7-O-β-D-glucoside and biochanin A are the major components of the CPE. Gene expression of TNF-α, APP, BACE1, PSEN-2 and ER-β were assessed in brain extract using RT-PCR. Also, NF-кB subunit P65 and COX-2 expression were evaluated by western blotting. The cholinergic function, histological examination and oxidative status were also estimated. The AlCl 3 significantly up regulated the expression of the NF-кB subunit P65, COX-2, TNF- α, BACE1and APP while it significantly down regulated PSEN-2 and ER-β expression. The activity of acetyl cholinesterase (AChE) and the oxidative stress parameters as well as the histological examination confirmed the deleterious effect of AlCl 3. The administration of either CPE or Gen attenuated the expression of inflammatory cytokines, inhibited the amyloidogenesis and restored both the AChE activity and ER-β expression. Gen and CPE also inhibited the oxidative stress and ameliorated the histological alterations. Accordingly, the present study provides an insight on the molecular role of Gen and CPE as protective agents against neuronal injury.
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Genistein and chickpea ethanol extracts (10 mg/kg) mitigated AlCl3-induced neurotoxicity by reducing AChE activity, inflammation, and oxidative stress.
“genistein and chickpea ethanol extracts (10 mg/kg) mitigated AlCl3-induced neurotoxicity by reducing AChE activity, inflammation, and oxidative stress [67].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [67]
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Recent Advances in Medicinal Plant Research for Mitigating Aluminium Toxicity
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- Authors
- Wahby, M. M., Mohammed, D. S., Newairy, A. A., Abdou, H. M., & Zaky, A.
- Affiliations
- Alexandria University (EG)
- Corresponding author
- Wahby, M.M.
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 107
- Issue
- Pt A
- Pages
- 57-67
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2618988984
- Cited by
- 35
- References
- 77
- Citations by year
- 2026: 6 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 1
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- aluminum neurotoxicity, genistein, neuroprotection, oxidative stress, amyloidogenesis, NF-κB p65, cyclooxygenase-2, TNF-α, BACE1, APP, presenilin 2, estrogen receptor beta, acetylcholinesterase, biochanin A, neuroinflammation, Alzheimer's disease
- MeSH terms
- Aluminum, Animals, Humans, Male, Plant Extracts, Seeds, Tumor Necrosis Factor-alpha, NF-kappa B, Rats, Sprague-Dawley, Oxidative Stress, Neurodegenerative Diseases, Genistein, Protective Agents, Cicer, Rats, Cyclooxygenase 2
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