Cited by 36Aluminum toxicity and tolerance in plants and animalsMedicinal Plants and NeuroprotectionAlzheimer's disease research and treatments

Aluminum-induced molecular neurodegeneration: The protective role of genistein and chickpea extract

Wahby, M.M., Mohammed, D.S., Newairy, A.A., Abdou, H.M., Zaky, A.·2017·Food and Chemical Toxicology

Abstract

Neurotoxicity of Al is well established and linked to oxidative damage and neurodegeneration. This study investigated the protective role of genistein (Gen) and chickpea extract (CPE) against AlCl 3-induced neurodegeneration. HPLC analysis revealed that biochanin A-7-O-β-D-glucoside and biochanin A are the major components of the CPE. Gene expression of TNF-α, APP, BACE1, PSEN-2 and ER-β were assessed in brain extract using RT-PCR. Also, NF-кB subunit P65 and COX-2 expression were evaluated by western blotting. The cholinergic function, histological examination and oxidative status were also estimated. The AlCl 3 significantly up regulated the expression of the NF-кB subunit P65, COX-2, TNF- α, BACE1and APP while it significantly down regulated PSEN-2 and ER-β expression. The activity of acetyl cholinesterase (AChE) and the oxidative stress parameters as well as the histological examination confirmed the deleterious effect of AlCl 3. The administration of either CPE or Gen attenuated the expression of inflammatory cytokines, inhibited the amyloidogenesis and restored both the AChE activity and ER-β expression. Gen and CPE also inhibited the oxidative stress and ameliorated the histological alterations. Accordingly, the present study provides an insight on the molecular role of Gen and CPE as protective agents against neuronal injury.

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Crossref · 10.1016/j.fct.2017.05.044

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Authors
Wahby, M. M., Mohammed, D. S., Newairy, A. A., Abdou, H. M., & Zaky, A.
Affiliations
Alexandria University (EG)
Corresponding author
Wahby, M.M.
Venue
Food and Chemical Toxicology
Publisher
Elsevier BV
Published
2017-09
Volume
107
Issue
Pt A
Pages
57-67
ISSN
0278-6915 (ISSN-L)
OpenAlex
W2618988984
Cited by
35
References
77
Citations by year
2026: 6 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 1
FWCI
3.23
Type
journal article
Language
en
Open access
closed
Locations
  • Food and Chemical Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
aluminum neurotoxicity, genistein, neuroprotection, oxidative stress, amyloidogenesis, NF-κB p65, cyclooxygenase-2, TNF-α, BACE1, APP, presenilin 2, estrogen receptor beta, acetylcholinesterase, biochanin A, neuroinflammation, Alzheimer's disease
MeSH terms
Aluminum, Animals, Humans, Male, Plant Extracts, Seeds, Tumor Necrosis Factor-alpha, NF-kappa B, Rats, Sprague-Dawley, Oxidative Stress, Neurodegenerative Diseases, Genistein, Protective Agents, Cicer, Rats, Cyclooxygenase 2

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