Nicotine affects rat Leydig cell function in vivo and vitro via down-regulating some key steroidogenic enzyme expressions
Abstract
Nicotine is consumed largely as a component of cigarettes and has a potential effect on pubertal development of Leydig cells in males. To investigate its effects, 49-day-old male Sprague Dawley rats received intraperitoneal injections of nicotine (0.5 or 1 mg/kg/day) for 2 weeks and immature Leydig cells were isolated from the testes of 35-day-old rats and treated with nicotine (0.05-50 μM). Serum hormones, Leydig cell number and related gene expression levels after in vivo treatment were determined and medium androgen levels were measured and cell cycle, apoptosis, mitochondrial membrane potential (△Ψm), and reactive oxygen species (ROS) of Leydig cells after in vitro treatment were measured. In vivo exposure to nicotine lowered serum luteinizing hormone, follicle stimulating hormone, and testosterone levels and reduced Leydig cell number and gene expression levels. Nicotine in vitro inhibited androgen production in Leydig cells by downregulating the expression levels of P450 cholesterol side cleavage enzyme, 3β-hydroxysteroid dehydrogenase 1, and steroidogenic factor 1 at different concentration ranges. In conclusion, nicotine disrupts Leydig cell steroidogenesis during puberty possibly via down-regulating some key steroidogenic enzyme expressions.
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- Authors
- Guo, X., Wang, H., Wu, X., Chen, X., Chen, Y., Guo, J., Li, X., Lian, Q., & Ge, R.-S.
- Affiliations
- Wenzhou Medical University (CN)
- Corresponding author
- Lian, Qingquan
- Ge, Ren-Shan
- ORCID
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 110
- Pages
- 13-24
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2763056011
- Cited by
- 38
- References
- 42
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 10 · 2018: 4
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Health & Family Planning Commission of Zhejiang Province (2017KY473, 11-CX29)
- Zhejiang Provincial NSF (LQ16H040005)
- Wenzhou Science & Technology Bureau (Y20170151, Y20140661)
- NSFC (81701426, 81300471, 81601266, 81601264)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- nicotine, Leydig cells, steroidogenesis, steroidogenic enzymes, puberty, testosterone, luteinizing hormone, follicle-stimulating hormone, steroidogenic factor 1, CYP11A1, androgen production, apoptosis, cell cycle, mitochondrial membrane potential, reactive oxygen species, testis
- MeSH terms
- Androgens, Animals, Cholesterol Side-Chain Cleavage Enzyme, Follicle Stimulating Hormone, Leydig Cells, Luteinizing Hormone, Male, Nicotine, Steroid Isomerases, Testosterone, Down-Regulation, Rats, Sprague-Dawley, Cell Proliferation
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