CoChat
Sign Up
Cited by 35

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

In vitro assessment of silver nanoparticles immunotoxicity

Galbiati, Valentina, Cornaghi, Laura, Gianazza, Elisabetta, Potenza, Marco A., Donetti, Elena, Marinovich, Marina, Corsini, Emanuela·2018·Food and Chemical Toxicology
DOI 10.1016/j.fct.2017.12.023
Open this paper in CoChat — free account

Abstract

This study aimed to characterize unwanted immune effects of nanoparticles (NP) using THP-1 cells, human whole blood and enriched peripheral blood monocytes. Commercially available silver NP (AgNP < 100 nm, also confirmed by Single Particle Extinction and Scattering) were used as prototypical NP. Cells were treated with AgNP alone or in combination with classical immune stimuli (i.e. LPS, PHA, PWM) and cytokine assessed; in addition, CD54 and CD86 expression was evaluated in THP-1 cells. AgNP alone induced dose-related IL-8 production in all models, with higher response observed in THP-1 cells, possibly connected to different protein corona formation in bovine versus human serum. AgNP potentiated LPS-induced IL-8 and TNF-α, but not LPS-induced IL-10. AgNP alone induced slight increase in IL-4, and no change in IFN-γ production. While responses to PHA in term of IL-4 and IFN-γ production were not affected, increased PWM-induced IL-4 and IFN-γ production were observed, suggesting potentiation of humoral response. Reduction in PHA-induced IL-10 was observed. Overall, results indicate immunostimulatory effects. THP-1 cells work as well as primary cells, representing a useful and practical alternative, with the awareness that from a physiological point of view the whole blood assay is the one that comes closest to reality.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.fct.2017.12.023

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 2 references

Research works printed but not linked: 67

  1. Bailey, 2013, A simple whole blood bioassay detects cytokine responses to anti-CD28SA and anti-CD52 antibodies, J. Pharmacol. Toxicol. Methods, 68, 231, 10.1016/j.vascn.2012.12.003 · DOI
  2. Bekeredjian-Ding, 2012, Poke weed mitogen requires Toll-like receptor ligands for proliferative activity in human and murine B lymphocytes, PLoS One, 7, 10.1371/journal.pone.0029806 · DOI
  3. Bosshart, 2016, THP-1 cells as a model for human monocytes, Ann. Transl. Med., 4, 438, 10.21037/atm.2016.08.53 · DOI
  4. Chanput, 2014, THP-1 cell line: an in vitro cell model for immune modulation approach, Int. Immunopharmacol., 23, 37, 10.1016/j.intimp.2014.08.002 · DOI
  5. Corsini, 2010, Evaluating cytokines in immunotoxicity testing, Methods Mol. Biol., 598, 283, 10.1007/978-1-60761-401-2_20 · DOI
  6. Corti, 2015, Optimization of the THP-1 activation assay to detect pharmaceuticals with potential to cause immune mediated drug reactions, Toxicol. In Vitro, 29, 1339, 10.1016/j.tiv.2015.04.012 · DOI
  7. De Jong, 2013, Systemic and immunotoxicity of silver nanoparticles in an intravenous 28 days repeated dose toxicity study in rats, Biomaterials, 34, 8333, 10.1016/j.biomaterials.2013.06.048 · DOI
  8. Devanabanda, 2016, Immunotoxic effects of gold and silver nanoparticles: inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytes in vitro, J. Immunotoxicol., 13, 897, 10.1080/1547691X.2016.1234522 · DOI
  9. Dobrovolskaia, 2009, Evaluation of nanoparticle immunotoxicity, Nat. Nanotechnol., 4, 411, 10.1038/nnano.2009.175 · DOI
  10. Dobrovolskaia, 2013, Understanding the correlation between in vitro and in vivo immunotoxicity tests for nanomedicines, J. Contr. Release, 172, 456, 10.1016/j.jconrel.2013.05.025 · DOI
  11. Dobrovolskaia, 2014, Protein corona composition does not accurately predict hematocompatibility of colloidal gold nanoparticles, Nanomedicine, 10, 1453, 10.1016/j.nano.2014.01.009 · DOI
  12. Dobrovolskaia, 2016, Current understanding of interactions between nanoparticles and the immune system, Toxicol. Appl. Pharmacol., 299, 78, 10.1016/j.taap.2015.12.022 · DOI
  13. Donaldson, 2005, Combustion-derived nanoparticles: a review of their toxicology following inhalation exposure, Part. Fibre Toxicol., 2, 10, 10.1186/1743-8977-2-10 · DOI
  14. Engin, 2017, Mechanistic understanding of nanoparticles' interactions with extracellular matrix: the cell and immune system, Part. Fibre Toxicol., 14, 22, 10.1186/s12989-017-0199-z · DOI
  15. Fröhlich, 2015, Value of phagocyte function screening for immunotoxicity of nanoparticles in vivo, Int. J. Nanomedicine, 10
  16. Fröhlich, 2016, Oral uptake of nanoparticles: human relevance and the role of in vitro systems, Arch. Toxicol., 90, 2297, 10.1007/s00204-016-1765-0 · DOI
  17. Galbiati, 2010, Present and future of in vitro immunotoxicology in drug development, J. Immunotoxicol., 7, 255, 10.3109/1547691X.2010.509848 · DOI
  18. Ge, 2014, Nanosilver particles in medical applications: synthesis, performance, and toxicity, Int. J. Nanomedicine, 9, 2399
  19. Genin, 2015, M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide, BMC Canc., 15, 577, 10.1186/s12885-015-1546-9 · DOI · Paper page
  20. Gennari, 2005, The use of in vitro systems for evaluating immunotoxicity: the report and recommendations of an ECVAM workshop, J. Immunotoxicol., 2, 61, 10.1080/15476910590965832 · DOI
  21. Ghosh, 2012, In vitro and in vivo genotoxicity of silver nanoparticles, Mutat. Res., 749, 60, 10.1016/j.mrgentox.2012.08.007 · DOI
  22. Giannakou, 2016, A comparison of immunotoxic effects of nanomedicinal products with regulatory immunotoxicity testing requirements, Int. J. Nanomedicine, 11, 2935, 10.2147/IJN.S102385 · DOI
  23. Gustafson, 2015, Nanoparticle uptake: the phagocyte problem, Nano Today, 10, 487, 10.1016/j.nantod.2015.06.006 · DOI
  24. Hadrup, 2014, Oral toxicity of silver ions, silver nanoparticles and colloidal silver–a review, Regul. Toxicol. Pharmacol., 68, 1, 10.1016/j.yrtph.2013.11.002 · DOI
  25. Halamoda-Kenzaoui, 2015, Dispersion behaviour of silica nanoparticles in biological media and its influence on cellular uptake, PLoS One, 10, 10.1371/journal.pone.0141593 · DOI
  26. Herd, 2015, Macrophage silica nanoparticle response is phenotypically dependent, Biomaterials, 53, 574, 10.1016/j.biomaterials.2015.02.070 · DOI
  27. Heukeshoven, 1988, Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels, Electrophoresis, 9, 28, 10.1002/elps.1150090106 · DOI
  28. Karmali, 2011, Interactions of nanoparticles with plasma proteins: implication on clearance and toxicity of drug delivery systems, Expet Opin. Drug Deliv., 8, 343, 10.1517/17425247.2011.554818 · DOI
  29. Kettler, 2016, Uptake of silver nanoparticles by monocytic THP-1 cells depends on particle size and presence of serum proteins, J. Nanopart. Res., 18, 286, 10.1007/s11051-016-3595-7 · DOI
  30. Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 · DOI · Paper page
  31. Lappas, 2015, The immunomodulatory effects of titanium dioxide and silver nanoparticles, Food Chem. Toxicol., 85, 78, 10.1016/j.fct.2015.05.015 · DOI
  32. Langezaal, 2002, Evaluation and prevalidation of an immunotoxicity test based on human whole-blood cytokine release, Altern. Lab. Anim., 30, 581, 10.1177/026119290203000605 · DOI
  33. Laufer, 2008, Human whole blood assay for rapid and routine testing of non-steroidal anti-inflammatory drugs (NSAIDs) on cyclo-oxygenase-2 activity, Inflammopharmacology, 16, 155, 10.1007/s10787-008-8007-x · DOI
  34. Lee, 2014, Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity, Int. J. Nanomedicine, 10, 97
  35. Lesniak, 2012, Effects of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells, ACS Nano, 6, 10.1021/nn300223w · DOI · Paper page
  36. Li, 2016, A work group report on ultrafine particles (American Academy of Allergy, Asthma & Immunology): why ambient ultrafine and engineered nanoparticles should receive special attention for possible adverse health outcomes in human subjects, J. Allergy Clin. Immunol., 138, 386, 10.1016/j.jaci.2016.02.023 · DOI
  37. Li, 2016, Assessing the immunosafety of engineered nanoparticles with a novel in vitro model based on human primary monocytes, ACS Appl. Mater. Interfaces, 8, 28437, 10.1021/acsami.6b06278 · DOI
  38. Lin, 2015, Pharmacokinetics of metallic nanoparticles, Wiley. Interdiscip. Rev. Nanomed. Nanobiotechnol., 7, 189, 10.1002/wnan.1304 · DOI
  39. Mendoza, 2014, Silica nanoparticles induce oxidative stress and inflammation of human peripheral blood mononuclear cells, Cell Stress Chaperones, 19, 777, 10.1007/s12192-014-0502-y · DOI
  40. Mitjans, 2008, Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naive THP-1 cells, Toxicol. In Vitro, 22, 386, 10.1016/j.tiv.2007.10.005 · DOI
  41. Mitjans, 2010, Use of IL-8 release and p38 MAPK activation in THP-1 cells to identify allergens and to assess their potency in vitro, Toxicol. In Vitro, 24, 1803, 10.1016/j.tiv.2010.06.001 · DOI
  42. Monopoli, 2012, Biomolecular coronas provide the biological identity of nanosized materials, Nat. Nanotechnol., 7, 779, 10.1038/nnano.2012.207 · DOI · Paper page
  43. Neagu, 2017, Protein bio-corona: critical issue in immune nanotoxicology, Arch. Toxicol., 91, 1031, 10.1007/s00204-016-1797-5 · DOI
  44. Nishijima, 2017, Human scavenger receptor A1-mediated inflammatory response to silica particle exposure is size specific, Front. Immunol., 8, 379, 10.3389/fimmu.2017.00379 · DOI
  45. Oostingh, 2011, Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects, Part. Fibre Toxicol., 8, 8, 10.1186/1743-8977-8-8 · DOI
  46. Pallardy, 2017, Why the immune system should Be concerned by nanomaterials?, Front. Immunol., 8, 544, 10.3389/fimmu.2017.00544 · DOI
  47. Petrarca, 2015, Engineered metal based nanoparticles and innate immunity, Clin. Mol. Allergy, 13, 13, 10.1186/s12948-015-0020-1 · DOI
  48. Piperigkou, 2016, Emerging aspects of nanotoxicology in health and disease: from agriculture and food sector to cancer therapeutics, Food Chem. Toxicol., 91, 42, 10.1016/j.fct.2016.03.003 · DOI
  49. Potenza, 2015, Measuring the complex field scattered by single submicron particles, AIP Adv., 5, 117222, 10.1063/1.4935927 · DOI
  50. Potenza, 2015, Single particle optical extinction and scattering allows real time quantitative characterization of drug payload and degradation of polymeric nanoparticles, Sci. Rep., 5, 18228, 10.1038/srep18228 · DOI
  51. Potenza, 2017, Detecting the shape of anisotropic gold nanoparticles in dispersion with single particle extinction and scattering, Nanoscale, 9, 2778, 10.1039/C6NR08977A · DOI
  52. Prietl, 2014, Nano-sized and micro-sized polystyrene particles affect phagocyte function, Cell Biol. Toxicol., 30, 1, 10.1007/s10565-013-9265-y · DOI
  53. Rahman, 2013, Nanoparticle and protein corona, vol. 15, 21
  54. Remick, 2000, Whole-blood assays for cytokine production, Methods Mol. Med., 36, 101
  55. Salatin, 2015, Effect of the surface modification, size, and shape on cellular uptake of nanoparticles, Cell Biol. Int., 39, 881, 10.1002/cbin.10459 · DOI
  56. Shannahan, 2016, From the cover: disease-induced disparities in formation of the nanoparticle-biocorona and the toxicological consequences, Toxicol. Sci., 152, 406, 10.1093/toxsci/kfw097 · DOI
  57. Shishatskaya, 2016, Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use, Food Chem. Toxicol., 96, 302, 10.1016/j.fct.2016.08.025 · DOI
  58. Shin, 2007, The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells, Int. Immunopharmacol., 7, 1813, 10.1016/j.intimp.2007.08.025 · DOI
  59. Shvedova, 2016, Nanotoxicology ten years later: lights and shadows, Toxicol. Appl. Pharmacol., 299, 1, 10.1016/j.taap.2016.02.014 · DOI
  60. Silva, 2013, A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens, Acta Trop., 127, 75, 10.1016/j.actatropica.2013.04.002 · DOI
  61. Stevenson, 1983, Interaction of pokeweed mitogen with monocytes in the activation of human lymphocytes, Immunology, 49, 633
  62. Suzuki, 2007, Simple and easy method to evaluate uptake potential of nanoparticles in mammalian cells using a flow cytometric light scatter analysis, Environ. Sci. Technol., 41, 3018, 10.1021/es0625632 · DOI
  63. Toebak, 2006, CXCL8 secretion by dendritic cells predicts contact allergens from irritants, Toxicol. In Vitro, 20, 117, 10.1016/j.tiv.2005.06.039 · DOI
  64. Tulve, 2015, Characterization of silver nanoparticles in selected consumer products and its relevance for predicting children's potential exposures, Int. J. Hyg Environ. Health, 218, 345, 10.1016/j.ijheh.2015.02.002 · DOI
  65. Xu, 2013, Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo, Toxicol. Lett., 219, 42, 10.1016/j.toxlet.2013.02.010 · DOI
  66. Yan, 2013, Differential roles of the protein corona in the cellular uptake of nanoporous polymer particles by monocyte and macrophage cell lines, ACS Nano, 7, 10960, 10.1021/nn404481f · DOI
  67. Zhang, 2011, Size-dependent in vivo toxicity of PEG-coated gold nanoparticles, Int J Nanomedicine, 6, 2071, 10.2147/IJN.S21657 · DOI

Cited by

Sorted by citations

See all 35

Metadata

Authors
Galbiati, V., Cornaghi, L., Gianazza, E., Potenza, M. A., Donetti, E., Marinovich, M., & Corsini, E.
Venue
Food and Chemical Toxicology
Publisher
Elsevier BV
Published
2018-02
Volume
112
Pages
363-374
ISSN
0278-6915
DOI
10.1016/j.fct.2017.12.023
Cited by
35
References
2
Language
en
Licence
Funders
Università degli Studi di Milano (2015/2017)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: In vitro assessment of silver nanoparticles immunotoxicity. https://app.cochat.ai/papers/10.1016/j.fct.2017.12.023
The paper
Galbiati, V., Cornaghi, L., Gianazza, E., Potenza, M. A., Donetti, E., Marinovich, M., & Corsini, E. (2018). In vitro assessment of silver nanoparticles immunotoxicity. Food and Chemical Toxicology. https://doi.org/10.1016/j.fct.2017.12.023